Matches in SemOpenAlex for { <https://semopenalex.org/work/W2572764581> ?p ?o ?g. }
Showing items 1 to 86 of
86
with 100 items per page.
- W2572764581 endingPage "8" @default.
- W2572764581 startingPage "1" @default.
- W2572764581 abstract "Chalcone synthase ( CHS ) is a key enzyme in the flavonoid biosynthesis pathway. CHS genes were cloned from genomic DNA and cDNA from the petals of ' Buntharik ' white lotus and ' Sattabangkacha ' pink lotus by the PCR technique using a specific primer of the CHS gene designed from the GenBank database. Semi-quantitative RT-PCR analysis revealed that the highest CHS gene expression was found in the early budding stage of the pink lotus and was reduced in later stages. Shoot tips from embryos of Buntharik and Rachinee lotus were used to induce shoot clusters by cultivation on a MS medium supplemented with 40 µM NAA and 0.5 µM TDZ for 8 weeks and a MS medium supplemented with 50 µM BA for 8 weeks. An antisense CHS gene (450 bp) from the cDNA of Buntharik lotus was used to construct a plant transformation vector; pCAMBIA1302CHSA. The vector construct was transformed into Buntharik and Rachinee shoot clusters by particle bombardment. After transformant selection and regeneration, two transformants of Buntharik shoot clusters showed GFP green spots and existence of the GFP gene and hpt II gene in the genomic DNA amplified by the PCR technique. In the Rachinee transformants, 3 of 5 showed the GFP green spots and the GFP and hpt II genes were identified in amplification by PCR. After CHS gene expression analyses by semi-quantitative RT-PCR, two transformed Rachinee shoot clusters had a reduction in CHS gene expression." @default.
- W2572764581 created "2017-01-26" @default.
- W2572764581 creator A5036848226 @default.
- W2572764581 creator A5053338356 @default.
- W2572764581 creator A5071427162 @default.
- W2572764581 creator A5079921532 @default.
- W2572764581 date "2017-01-06" @default.
- W2572764581 modified "2023-09-25" @default.
- W2572764581 title "Transformation of Antisense Chalcone Synthase (CHS) Gene into Lotus (Nelumbo Nucifera Gaertn.) by Particle Bombardment" @default.
- W2572764581 cites W1982550757 @default.
- W2572764581 cites W1985418187 @default.
- W2572764581 cites W1992255794 @default.
- W2572764581 cites W1993232387 @default.
- W2572764581 cites W1998746895 @default.
- W2572764581 cites W2010863475 @default.
- W2572764581 cites W2016622993 @default.
- W2572764581 cites W2018170869 @default.
- W2572764581 cites W2024556021 @default.
- W2572764581 cites W2075664438 @default.
- W2572764581 cites W2109010920 @default.
- W2572764581 cites W2113373428 @default.
- W2572764581 cites W2127497651 @default.
- W2572764581 cites W2589506964 @default.
- W2572764581 cites W4231255281 @default.
- W2572764581 cites W4243890786 @default.
- W2572764581 doi "https://doi.org/10.2174/1874070701710011001" @default.
- W2572764581 hasPublicationYear "2017" @default.
- W2572764581 type Work @default.
- W2572764581 sameAs 2572764581 @default.
- W2572764581 citedByCount "1" @default.
- W2572764581 countsByYear W25727645812019 @default.
- W2572764581 crossrefType "journal-article" @default.
- W2572764581 hasAuthorship W2572764581A5036848226 @default.
- W2572764581 hasAuthorship W2572764581A5053338356 @default.
- W2572764581 hasAuthorship W2572764581A5071427162 @default.
- W2572764581 hasAuthorship W2572764581A5079921532 @default.
- W2572764581 hasBestOaLocation W25727645811 @default.
- W2572764581 hasConcept C104317684 @default.
- W2572764581 hasConcept C143065580 @default.
- W2572764581 hasConcept C150194340 @default.
- W2572764581 hasConcept C153911025 @default.
- W2572764581 hasConcept C17757408 @default.
- W2572764581 hasConcept C187882448 @default.
- W2572764581 hasConcept C204241405 @default.
- W2572764581 hasConcept C2777135195 @default.
- W2572764581 hasConcept C2777635637 @default.
- W2572764581 hasConcept C2779688875 @default.
- W2572764581 hasConcept C54355233 @default.
- W2572764581 hasConcept C59822182 @default.
- W2572764581 hasConcept C79029880 @default.
- W2572764581 hasConcept C86803240 @default.
- W2572764581 hasConceptScore W2572764581C104317684 @default.
- W2572764581 hasConceptScore W2572764581C143065580 @default.
- W2572764581 hasConceptScore W2572764581C150194340 @default.
- W2572764581 hasConceptScore W2572764581C153911025 @default.
- W2572764581 hasConceptScore W2572764581C17757408 @default.
- W2572764581 hasConceptScore W2572764581C187882448 @default.
- W2572764581 hasConceptScore W2572764581C204241405 @default.
- W2572764581 hasConceptScore W2572764581C2777135195 @default.
- W2572764581 hasConceptScore W2572764581C2777635637 @default.
- W2572764581 hasConceptScore W2572764581C2779688875 @default.
- W2572764581 hasConceptScore W2572764581C54355233 @default.
- W2572764581 hasConceptScore W2572764581C59822182 @default.
- W2572764581 hasConceptScore W2572764581C79029880 @default.
- W2572764581 hasConceptScore W2572764581C86803240 @default.
- W2572764581 hasIssue "1" @default.
- W2572764581 hasLocation W25727645811 @default.
- W2572764581 hasOpenAccess W2572764581 @default.
- W2572764581 hasPrimaryLocation W25727645811 @default.
- W2572764581 hasRelatedWork W2056172754 @default.
- W2572764581 hasRelatedWork W2068293420 @default.
- W2572764581 hasRelatedWork W2357632800 @default.
- W2572764581 hasRelatedWork W2364221357 @default.
- W2572764581 hasRelatedWork W2375596579 @default.
- W2572764581 hasRelatedWork W2385928657 @default.
- W2572764581 hasRelatedWork W2393307878 @default.
- W2572764581 hasRelatedWork W2404499195 @default.
- W2572764581 hasRelatedWork W615208977 @default.
- W2572764581 hasRelatedWork W2282737375 @default.
- W2572764581 hasVolume "11" @default.
- W2572764581 isParatext "false" @default.
- W2572764581 isRetracted "false" @default.
- W2572764581 magId "2572764581" @default.
- W2572764581 workType "article" @default.