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- W2572839717 abstract "Stevia is a natural sweetener with increasing use in known products, for instance Coca Cola Company launched a new brand called Life were they use Stevia as a sweetener. In this work, we have attempted to separate and analyze the different compounds that give the sweet taste, known as steviol glycosides, by ion-exchange chromatography with pulsed amperometric detection. The known methods for separating rebaudiosides often use HPLC and thereby often use polar interaction to separate the compounds. No research has been found where High-Performance Anion Exchange Chromatography with Pulsed Amperometric Detector (HPAEC-PAD) is used to separate the steviol glycosides, or as some of them are called, rebaudiosides. In this work the sugar moieties, attached to a non-polar diterpene as aglycone, were ionized in highly alkaline mobile phase and separated on a CarboPac PA20 column. This use of the sugar moieties for separation have not been used before and proved to give a good separation. The resin of this column is non-polar and interacted with the diterpene part of the rebaudiosides, there for acetonitrile was added to the mobile phase to counteract the hydrophobic interaction between these two parts. The exploratory experiments showed that acetonitrile does reduce the hydrophobic interaction and that the rebaudioside A, used as standard, does also have ionic interaction with the column and could be eluted with 200 mM NaOH, gradient of 0-200 mM NaOAc and 10 % ACN. A commercial product with stevioside and rebaudioside A are run under same conditions and achieves a good separation, thereby opening for further investigation of the separation of steviol glycosides with anion exchange chromatography. (Less)" @default.
- W2572839717 created "2017-01-26" @default.
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- W2572839717 date "2016-01-01" @default.
- W2572839717 modified "2023-09-27" @default.
- W2572839717 title "Explorative experiments on separation of Steviol Glycosides with Ion-Exchange Chromatography and Pulsed Amperometric Detector" @default.
- W2572839717 hasPublicationYear "2016" @default.
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