Matches in SemOpenAlex for { <https://semopenalex.org/work/W2573232933> ?p ?o ?g. }
- W2573232933 endingPage "e1006132" @default.
- W2573232933 startingPage "e1006132" @default.
- W2573232933 abstract "Ebola (EBOV) and Marburg (MARV) viruses are members of the Filoviridae family which cause outbreaks of hemorrhagic fever. The filovirus VP40 matrix protein is essential for virus assembly and budding, and its PPxY L-domain motif interacts with WW-domains of specific host proteins, such as Nedd4 and ITCH, to facilitate the late stage of virus-cell separation. To identify additional WW-domain-bearing host proteins that interact with VP40, we used an EBOV PPxY-containing peptide to screen an array of 115 mammalian WW-domain-bearing proteins. Using this unbiased approach, we identified BCL2 Associated Athanogene 3 (BAG3), a member of the BAG family of molecular chaperone proteins, as a specific VP40 PPxY interactor. Here, we demonstrate that the WW-domain of BAG3 interacts with the PPxY motif of both EBOV and MARV VP40 and, unexpectedly, inhibits budding of both eVP40 and mVP40 virus-like particles (VLPs), as well as infectious VSV-EBOV recombinants. BAG3 is a stress induced protein that regulates cellular protein homeostasis and cell survival through chaperone-mediated autophagy (CMA). Interestingly, our results show that BAG3 alters the intracellular localization of VP40 by sequestering VP40 away from the plasma membrane. As BAG3 is the first WW-domain interactor identified that negatively regulates budding of VP40 VLPs and infectious virus, we propose that the chaperone-mediated autophagy function of BAG3 represents a specific host defense strategy to counteract the function of VP40 in promoting efficient egress and spread of virus particles." @default.
- W2573232933 created "2017-01-26" @default.
- W2573232933 creator A5001008905 @default.
- W2573232933 creator A5017944089 @default.
- W2573232933 creator A5030549908 @default.
- W2573232933 creator A5044259899 @default.
- W2573232933 creator A5053626350 @default.
- W2573232933 creator A5058298248 @default.
- W2573232933 creator A5063601670 @default.
- W2573232933 date "2017-01-11" @default.
- W2573232933 modified "2023-09-27" @default.
- W2573232933 title "Chaperone-Mediated Autophagy Protein BAG3 Negatively Regulates Ebola and Marburg VP40-Mediated Egress" @default.
- W2573232933 cites W135710278 @default.
- W2573232933 cites W1590083128 @default.
- W2573232933 cites W1606037334 @default.
- W2573232933 cites W174731117 @default.
- W2573232933 cites W1783583538 @default.
- W2573232933 cites W1973858755 @default.
- W2573232933 cites W1979629343 @default.
- W2573232933 cites W1980118923 @default.
- W2573232933 cites W1980818815 @default.
- W2573232933 cites W1986482237 @default.
- W2573232933 cites W1987857828 @default.
- W2573232933 cites W1990916105 @default.
- W2573232933 cites W2004506660 @default.
- W2573232933 cites W2006820159 @default.
- W2573232933 cites W2006838403 @default.
- W2573232933 cites W2007534169 @default.
- W2573232933 cites W2008316672 @default.
- W2573232933 cites W2009409181 @default.
- W2573232933 cites W2012521475 @default.
- W2573232933 cites W2015338902 @default.
- W2573232933 cites W2015908833 @default.
- W2573232933 cites W2018741273 @default.
- W2573232933 cites W2023215532 @default.
- W2573232933 cites W2033152875 @default.
- W2573232933 cites W2034662297 @default.
- W2573232933 cites W2036721131 @default.
- W2573232933 cites W2045257543 @default.
- W2573232933 cites W2049480511 @default.
- W2573232933 cites W2053437843 @default.
- W2573232933 cites W2054065176 @default.
- W2573232933 cites W2054481485 @default.
- W2573232933 cites W2058382564 @default.
- W2573232933 cites W2059023146 @default.
- W2573232933 cites W2059814917 @default.
- W2573232933 cites W2060380070 @default.
- W2573232933 cites W2061195173 @default.
- W2573232933 cites W2070063935 @default.
- W2573232933 cites W2071559289 @default.
- W2573232933 cites W2071967966 @default.
- W2573232933 cites W2082144393 @default.
- W2573232933 cites W2082637970 @default.
- W2573232933 cites W2084299788 @default.
- W2573232933 cites W2096284021 @default.
- W2573232933 cites W2097854637 @default.
- W2573232933 cites W2113035264 @default.
- W2573232933 cites W2113259069 @default.
- W2573232933 cites W2125670962 @default.
- W2573232933 cites W2127806688 @default.
- W2573232933 cites W2130138387 @default.
- W2573232933 cites W2134325711 @default.
- W2573232933 cites W2145135253 @default.
- W2573232933 cites W2146508102 @default.
- W2573232933 cites W2148191268 @default.
- W2573232933 cites W2148258346 @default.
- W2573232933 cites W2150249332 @default.
- W2573232933 cites W2152523857 @default.
- W2573232933 cites W2156685362 @default.
- W2573232933 cites W2159223336 @default.
- W2573232933 cites W2159584227 @default.
- W2573232933 cites W2160504953 @default.
- W2573232933 cites W2164680606 @default.
- W2573232933 cites W2168673443 @default.
- W2573232933 cites W2171417214 @default.
- W2573232933 cites W2171854693 @default.
- W2573232933 cites W2474610049 @default.
- W2573232933 cites W4231619280 @default.
- W2573232933 cites W625383729 @default.
- W2573232933 doi "https://doi.org/10.1371/journal.ppat.1006132" @default.
- W2573232933 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5517035" @default.
- W2573232933 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28723972" @default.
- W2573232933 hasPublicationYear "2017" @default.
- W2573232933 type Work @default.
- W2573232933 sameAs 2573232933 @default.
- W2573232933 citedByCount "37" @default.
- W2573232933 countsByYear W25732329332017 @default.
- W2573232933 countsByYear W25732329332018 @default.
- W2573232933 countsByYear W25732329332019 @default.
- W2573232933 countsByYear W25732329332020 @default.
- W2573232933 countsByYear W25732329332021 @default.
- W2573232933 countsByYear W25732329332022 @default.
- W2573232933 countsByYear W25732329332023 @default.
- W2573232933 crossrefType "journal-article" @default.
- W2573232933 hasAuthorship W2573232933A5001008905 @default.
- W2573232933 hasAuthorship W2573232933A5017944089 @default.
- W2573232933 hasAuthorship W2573232933A5030549908 @default.
- W2573232933 hasAuthorship W2573232933A5044259899 @default.