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- W2573680128 abstract "The time course of calcium-activated chloride currents (f rail) in single cells of the rabbit portal vein was studied. These currents were activated by the influx of calcium through voltage-dependent calcium channels (VDCCs). At —50 mV, I ran decayed exponential- ly with a time constant (') of 80-100 ms that was inde- pendent of amplitude and was similar to the r of the de- cay of spontaneous transient inward currents (STICs; calcium-activated chloride currents). The decays of the STIC and 'tail had a similar voltage dependence between —50 and —110 mV and were similarly affected by the chloride channel blocker, niflumic acid. However, at more positive potentials (-20 to +40 mV), bail was sus- tained for the duration of the test pulse in most cells, in contrast to STICs which decayed exponentially. At very positive potentials (e.g. +100 mV), when little calcium enters the cell through VDCCs, Trail decayed exponential- ly. Measurement of calcium current (I ca ) at various po- tentials showed that the VDCCs did not inactivate fully at potentials between —20 and +30 mV. We propose that at negative potentials the decay of I tail is determined by slow gating of the chloride channel, but at positive po- tentials a sustained 'tail is produced by persistent influx of calcium through non-inactivating VDCCs." @default.
- W2573680128 created "2017-01-26" @default.
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- W2573680128 date "1996-01-01" @default.
- W2573680128 modified "2023-09-27" @default.
- W2573680128 title "Analysis of the time course of calcium-activated chloride tail currents in rabbit portal vein smooth muscle cells" @default.
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