FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2573854140> ?p ?o ?g. }

• W2573854140 abstract "Trematodicidal action of Marattia fraxinea (Sm.) rhizome, extracts against adult Gastrothylax crumenifer and its morphological changes under light and scanning electron microscope was studied in this work. This plant according to folk medicine has been reported to be used as antioxidant, antimicrobial and anthelmintic against ankylostomiasis. Preliminary screening involved the qualitative methods to detect the presence tannins, saponins, quinones, terpenoids, steroids, flavonoids, phenol, alkaloids, glycosides, cardiac glycosides, coumarins, anthocyanin and betacyanin. Total phenol, total terpenoids, total tannin and total flavonoids were quantitatively estimated. Total phenolic content was estimated by Folin- Ciocalteau method. Total flavonoid content was determined by the aluminium chloride colorimetric method. In-vitro incubation study of Marattia fraxinea Sm. extracts against Gastrothylax crumenifer were performed using 25 ml of Hedon-Fleig (H-F) salt solution containing various concentrations (1, 2, 3, 4 and 5 mg/ml) respectively as test extracts, positive control Oxyclozanide @ 1% (250 mg/25 ml) and negative control H-F salt solution, distributed to 7-Petri plates in an incubator with 5% CO2 at 37 °C. Twenty five amphistomes were incubated and the motility of control and test group was observed under dissection microscope at a regular time interval of (0, 10, 15, 30 and 60) min respectively. The motility response of the parasites was categorized with specific score 3, 2, 1, 0 respectively. Relative Motility (RM) value and Lethal Concentration 50 (LC50) were determined by probit regression analysis. Ultrastructure and histopathological changes on morphology of G.crumenifer were interpreted. The bioactive compounds were analysed using gas chromatography-mass spectrometry (GC-MS) instrument. Phytochemical analysis of M.fraxinea (Sm.) revealed the presence of secondary metabolites tannins, saponins, quinones, terpenoids, steroids, flavonoids, phenol, alkaloids, cardiac glycosides, coumarins, and betacyanin. Total terpenoid, total phenol, total tannin and total flavonoid content were found to be 16.00 ± 0.85 mg/g. 5.40 ± 0.71) mg GAE/g, 7.90 ± 0.24 mg TAE/g and 1.25 ± 0.41 mg QE/g respectively. GC-MS analysis showed presence of PHYTOL, a diterpenoids, fern-8-ene, a triterpenoids shown to have potent anthelmintic property. In vitro incubation study revealed death of all trematodes, lethal at 60 min incubation time at 5 mg/ml concentration, indicated Relative Motility (RM) value is 0, least effective compared to positive control Oxyclozanide, where RM value is 0 at 10 min. Lethal concentration 50 (LC50) was found to be 4.873 mg/ml. Confirmative study on gross morphology, histopathology, and ultra-structural changes showed considerable effect on suckers, teguments and internal organs and were dose dependent. It is indicative that presence of terpenoids, flavonoids, tannins and phenolics which are contributing factors for anthelmintic activity, producing various degree of damage to suckers, teguments and internal organs. Hence M. fraxinea (Sm.) can be a lead for synthesis of a new trematodicidal drug as alternative for other drugs and overcome anthelmintic resistance and cost effective." @default.
• W2573854140 created "2017-01-26" @default.
• W2573854140 creator A5010880210 @default.
• W2573854140 creator A5021569407 @default.
• W2573854140 creator A5033135649 @default.
• W2573854140 creator A5044121722 @default.
• W2573854140 creator A5048829191 @default.
• W2573854140 creator A5075684186 @default.
• W2573854140 creator A5079249063 @default.
• W2573854140 date "2017-01-17" @default.
• W2573854140 modified "2023-10-16" @default.
• W2573854140 title "Gastrothylax crumenifer: Ultrastructure and histopathology study of in vitro trematodicidal action of Marattia fraxinea (Sm.)" @default.
• W2573854140 cites W1696227661 @default.
• W2573854140 cites W1922392519 @default.
• W2573854140 cites W1964032462 @default.
• W2573854140 cites W1966069757 @default.
• W2573854140 cites W1966936556 @default.
• W2573854140 cites W1984232555 @default.
• W2573854140 cites W1993138943 @default.
• W2573854140 cites W1994743126 @default.
• W2573854140 cites W1994795924 @default.
• W2573854140 cites W2002117330 @default.
• W2573854140 cites W2015127479 @default.
• W2573854140 cites W2016013718 @default.
• W2573854140 cites W2025079886 @default.
• W2573854140 cites W2030181833 @default.
• W2573854140 cites W2034952306 @default.
• W2573854140 cites W2040783087 @default.
• W2573854140 cites W2050012552 @default.
• W2573854140 cites W2053346993 @default.
• W2573854140 cites W2058005680 @default.
• W2573854140 cites W2061703004 @default.
• W2573854140 cites W2066197312 @default.
• W2573854140 cites W2072610915 @default.
• W2573854140 cites W2074252192 @default.
• W2573854140 cites W2081907039 @default.
• W2573854140 cites W2086735810 @default.
• W2573854140 cites W2089839882 @default.
• W2573854140 cites W2089988804 @default.
• W2573854140 cites W2091876048 @default.
• W2573854140 cites W2092893785 @default.
• W2573854140 cites W2103231201 @default.
• W2573854140 cites W2122910156 @default.
• W2573854140 cites W2136016595 @default.
• W2573854140 cites W2140813769 @default.
• W2573854140 cites W2146212438 @default.
• W2573854140 cites W2155699161 @default.
• W2573854140 cites W2156814302 @default.
• W2573854140 cites W2327979819 @default.
• W2573854140 cites W2461674858 @default.
• W2573854140 cites W610094328 @default.
• W2573854140 doi "https://doi.org/10.1186/s40816-016-0039-y" @default.
• W2573854140 hasPublicationYear "2017" @default.
• W2573854140 type Work @default.
• W2573854140 sameAs 2573854140 @default.
• W2573854140 citedByCount "2" @default.
• W2573854140 countsByYear W25738541402021 @default.
• W2573854140 crossrefType "journal-article" @default.
• W2573854140 hasAuthorship W2573854140A5010880210 @default.
• W2573854140 hasAuthorship W2573854140A5021569407 @default.
• W2573854140 hasAuthorship W2573854140A5033135649 @default.
• W2573854140 hasAuthorship W2573854140A5044121722 @default.
• W2573854140 hasAuthorship W2573854140A5048829191 @default.
• W2573854140 hasAuthorship W2573854140A5075684186 @default.
• W2573854140 hasAuthorship W2573854140A5079249063 @default.
• W2573854140 hasBestOaLocation W25738541401 @default.
• W2573854140 hasConcept C105702510 @default.
• W2573854140 hasConcept C121332964 @default.
• W2573854140 hasConcept C142724271 @default.
• W2573854140 hasConcept C202751555 @default.
• W2573854140 hasConcept C2780791683 @default.
• W2573854140 hasConcept C544855455 @default.
• W2573854140 hasConcept C55493867 @default.
• W2573854140 hasConcept C62520636 @default.
• W2573854140 hasConcept C71924100 @default.
• W2573854140 hasConcept C86803240 @default.
• W2573854140 hasConcept C87555872 @default.
• W2573854140 hasConceptScore W2573854140C105702510 @default.
• W2573854140 hasConceptScore W2573854140C121332964 @default.
• W2573854140 hasConceptScore W2573854140C142724271 @default.
• W2573854140 hasConceptScore W2573854140C202751555 @default.
• W2573854140 hasConceptScore W2573854140C2780791683 @default.
• W2573854140 hasConceptScore W2573854140C544855455 @default.
• W2573854140 hasConceptScore W2573854140C55493867 @default.
• W2573854140 hasConceptScore W2573854140C62520636 @default.
• W2573854140 hasConceptScore W2573854140C71924100 @default.
• W2573854140 hasConceptScore W2573854140C86803240 @default.
• W2573854140 hasConceptScore W2573854140C87555872 @default.
• W2573854140 hasIssue "1" @default.
• W2573854140 hasLocation W25738541401 @default.
• W2573854140 hasOpenAccess W2573854140 @default.
• W2573854140 hasPrimaryLocation W25738541401 @default.
• W2573854140 hasRelatedWork W1984830004 @default.
• W2573854140 hasRelatedWork W2021603613 @default.
• W2573854140 hasRelatedWork W2079652959 @default.
• W2573854140 hasRelatedWork W2094838017 @default.
• W2573854140 hasRelatedWork W2475173856 @default.
• W2573854140 hasRelatedWork W4231103290 @default.
• W2573854140 hasRelatedWork W4240584934 @default.
• W2573854140 hasRelatedWork W4248854566 @default.

• next