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- W2573923 abstract "This study reports the physical characterization of a spin-labeled DNA complex designed by our lab. This DNA complex is to be used for future modeling studies on spin-spin interactions between the unpaired electrons of a spin-label and a metal center using Electron Paramagnetic Resonance (EPR). The model complex is composed of a synthetic, 19-base pair DNA duplex, which acts as the scaffold for a nitroxide radical (spin-label) and ethylenediaminetetraacetic acid (EDTA) that are covalently bonded to complementary strands. This model complex and its two derivative complexes, DNA duplexes containing only the nitroxide radical or the EDTA, were examined using Ultra-Violet (UV) and Circular Dichroism (CD) spectroscopic methods to determine their stability and conformation relative to that of the native DNA duplex. The melting temperatures, Tm, of the chemically modified duplexes were very similar to those of the native DNA duplex, and the CD studies of the modified duplexes indicated they have conformations similar to those of the B-form native duplex." @default.
- W2573923 created "2016-06-24" @default.
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- W2573923 date "2007-01-01" @default.
- W2573923 modified "2023-09-26" @default.
- W2573923 title "SPECTROSCOPIC CHARACTERIZATION OF DNA MODEL-SYSTEM" @default.
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