FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2575764448> ?p ?o ?g. }

• W2575764448 endingPage "1150" @default.
• W2575764448 startingPage "1140" @default.
• W2575764448 abstract "Matrix metalloproteinase (MMP)-14 is an important target for cancer treatment due to its critical roles in tumor invasion and metastasis. Previous failures of all compound-based broad-spectrum MMP inhibitors in clinical trials suggest that selectivity is the key for a successful therapy. With inherent high specificity, monoclonal antibodies (mAbs) therefore arise as attractive inhibitors able to target the particular MMP of interest. As a routine screening method, enzyme-linked immunosorbent assays (ELISA) have been applied to panned phage libraries for the isolation of mAbs inhibiting MMP-14. However, because of suboptimal growth conditions and insufficient antibody expression associated with monoclonal ELISA, a considerable number of potentially inhibitory clones might not be identified. Taking advantage of next-generation sequencing (NGS), we monitored enrichment profiles of millions of antibody clones along three rounds of phage panning, and identified 20 Fab inhibitors of MMP-14 with inhibition IC50 values of 10-4,000 nM. Among these inhibitory Fabs, 15 were not found by monoclonal phage ELISA. Particularly, Fab R2C7 exhibited an inhibition potency of 100 nM with an excellent selectivity to MMP-14 over MMP-9. Inhibition kinetics and epitope mapping suggested that as a competitive inhibitor, R2C7 directly bound to the vicinity of the MMP-14 catalytic site. This study demonstrates that deep sequencing is a powerful tool to facilitate the systematic discovery of mAbs with protease inhibition functions. Biotechnol. Bioeng. 2017;114: 1140-1150. © 2017 Wiley Periodicals, Inc." @default.
• W2575764448 created "2017-01-26" @default.
• W2575764448 creator A5014515622 @default.
• W2575764448 creator A5014563959 @default.
• W2575764448 creator A5020678188 @default.
• W2575764448 creator A5029271949 @default.
• W2575764448 creator A5062158929 @default.
• W2575764448 date "2017-02-20" @default.
• W2575764448 modified "2023-10-02" @default.
• W2575764448 title "Identification of highly selective MMP-14 inhibitory Fabs by deep sequencing" @default.
• W2575764448 cites W1491678226 @default.
• W2575764448 cites W1513230367 @default.
• W2575764448 cites W1968091806 @default.
• W2575764448 cites W1968280815 @default.
• W2575764448 cites W1971114436 @default.
• W2575764448 cites W1978591675 @default.
• W2575764448 cites W1980638845 @default.
• W2575764448 cites W1985731056 @default.
• W2575764448 cites W1993928780 @default.
• W2575764448 cites W1997299931 @default.
• W2575764448 cites W2003500892 @default.
• W2575764448 cites W2005334224 @default.
• W2575764448 cites W2007859267 @default.
• W2575764448 cites W2014393365 @default.
• W2575764448 cites W2020052113 @default.
• W2575764448 cites W2020972820 @default.
• W2575764448 cites W2030692069 @default.
• W2575764448 cites W2031495896 @default.
• W2575764448 cites W2036662231 @default.
• W2575764448 cites W2037042031 @default.
• W2575764448 cites W2038218681 @default.
• W2575764448 cites W2040166170 @default.
• W2575764448 cites W2053687002 @default.
• W2575764448 cites W2065513182 @default.
• W2575764448 cites W2073562518 @default.
• W2575764448 cites W2075503331 @default.
• W2575764448 cites W2084390276 @default.
• W2575764448 cites W2088514225 @default.
• W2575764448 cites W2091092639 @default.
• W2575764448 cites W2096487280 @default.
• W2575764448 cites W2097019319 @default.
• W2575764448 cites W2100695350 @default.
• W2575764448 cites W2109647778 @default.
• W2575764448 cites W2112782031 @default.
• W2575764448 cites W2116006352 @default.
• W2575764448 cites W2118526609 @default.
• W2575764448 cites W2128114769 @default.
• W2575764448 cites W2152135678 @default.
• W2575764448 cites W2155879201 @default.
• W2575764448 cites W2162498703 @default.
• W2575764448 cites W2283012379 @default.
• W2575764448 cites W2338685076 @default.
• W2575764448 cites W2566447341 @default.
• W2575764448 doi "https://doi.org/10.1002/bit.26248" @default.
• W2575764448 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5398909" @default.
• W2575764448 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28090632" @default.
• W2575764448 hasPublicationYear "2017" @default.
• W2575764448 type Work @default.
• W2575764448 sameAs 2575764448 @default.
• W2575764448 citedByCount "25" @default.
• W2575764448 countsByYear W25757644482017 @default.
• W2575764448 countsByYear W25757644482018 @default.
• W2575764448 countsByYear W25757644482019 @default.
• W2575764448 countsByYear W25757644482020 @default.
• W2575764448 countsByYear W25757644482021 @default.
• W2575764448 countsByYear W25757644482022 @default.
• W2575764448 countsByYear W25757644482023 @default.
• W2575764448 crossrefType "journal-article" @default.
• W2575764448 hasAuthorship W2575764448A5014515622 @default.
• W2575764448 hasAuthorship W2575764448A5014563959 @default.
• W2575764448 hasAuthorship W2575764448A5020678188 @default.
• W2575764448 hasAuthorship W2575764448A5029271949 @default.
• W2575764448 hasAuthorship W2575764448A5062158929 @default.
• W2575764448 hasBestOaLocation W25757644482 @default.
• W2575764448 hasConcept C104317684 @default.
• W2575764448 hasConcept C108944566 @default.
• W2575764448 hasConcept C109523444 @default.
• W2575764448 hasConcept C124913957 @default.
• W2575764448 hasConcept C151730666 @default.
• W2575764448 hasConcept C15336307 @default.
• W2575764448 hasConcept C153911025 @default.
• W2575764448 hasConcept C159654299 @default.
• W2575764448 hasConcept C167625842 @default.
• W2575764448 hasConcept C181199279 @default.
• W2575764448 hasConcept C185592680 @default.
• W2575764448 hasConcept C186268636 @default.
• W2575764448 hasConcept C195616568 @default.
• W2575764448 hasConcept C202751555 @default.
• W2575764448 hasConcept C203014093 @default.
• W2575764448 hasConcept C2776471321 @default.
• W2575764448 hasConcept C2776714187 @default.
• W2575764448 hasConcept C2777752497 @default.
• W2575764448 hasConcept C2779281246 @default.
• W2575764448 hasConcept C542903549 @default.
• W2575764448 hasConcept C55493867 @default.
• W2575764448 hasConcept C70721500 @default.
• W2575764448 hasConcept C86803240 @default.
• W2575764448 hasConceptScore W2575764448C104317684 @default.

• next