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• W2576068206 abstract "Recent advances in diagnostics have led to prospective HFA assays that are based on high-sensitivity immunoassay procedures, surface plasmon resonance, electrochemical detection, signal enhancement, and smartphone readout. There is an extensive need for assays that can simultaneously determine free HFA and HFA in calciprotein particles, which is challenging and would require highly innovative bioanalytical technologies. An emerging trend is strongly focused on developing miniaturized, fully integrated, and smartphone readout-based point-of-care testing format for HFA detection, which can be employed for diagnosing and managing diseases. Emerging assay formats, such as wash-free immunoassays, nanoparticle-based naked-eye formats, and paper-based assays, might lead to the development of rapid and highly sensitive HFA immunoassays. Human fetuin A (HFA) plays a prominent pathophysiological role in numerous diseases and pathophysiological conditions with considerable biomedical significance; one example is the formation of calciprotein particles in osteoporosis and impaired calcium metabolisms. With impressive advances in in vitro diagnostic assays during the last decade, ELISAs have become a workhorse in routine clinical diagnostics. Recent diagnostic formats involve high-sensitivity immunoassay procedures, surface plasmon resonance, rapid immunoassay chemistries, signal enhancement, and smartphone detection. The current trend is toward fully integrated lab-on-chip platforms with smartphone readouts, enabling health-care practitioners and even patients to monitor pathological changes in biomarker levels. This review provides a critical analysis of advances made in HFA assays along with the challenges and future prospects. Human fetuin A (HFA) plays a prominent pathophysiological role in numerous diseases and pathophysiological conditions with considerable biomedical significance; one example is the formation of calciprotein particles in osteoporosis and impaired calcium metabolisms. With impressive advances in in vitro diagnostic assays during the last decade, ELISAs have become a workhorse in routine clinical diagnostics. Recent diagnostic formats involve high-sensitivity immunoassay procedures, surface plasmon resonance, rapid immunoassay chemistries, signal enhancement, and smartphone detection. The current trend is toward fully integrated lab-on-chip platforms with smartphone readouts, enabling health-care practitioners and even patients to monitor pathological changes in biomarker levels. This review provides a critical analysis of advances made in HFA assays along with the challenges and future prospects." @default.
• W2576068206 created "2017-01-26" @default.
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• W2576068206 date "2017-05-01" @default.
• W2576068206 modified "2023-10-18" @default.
• W2576068206 title "Emerging Human Fetuin A Assays for Biomedical Diagnostics" @default.
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• W2576068206 doi "https://doi.org/10.1016/j.tibtech.2016.12.006" @default.
• W2576068206 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28094081" @default.
• W2576068206 hasPublicationYear "2017" @default.
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