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- W2576446190 abstract "The Nucleolar Organizer Region (NOR) was studied in Allium cepa. The cells analyzed were: meristematic root tip cells, cells from the layers of the anther (exotheeium, intermediate layer cells, endothecium and tapetum) and meiocytes from preleptotene to diptotene. Conventional electron microscope techniques and serial section were used in the study. Ultracytochemieal tests, using preferential techniques for nucleic acids were also applied. The results show that: a) Although the NOt~ may have a very variable form in the different cells analyzed, in each case its fine structure is similar, being constituted of chromatin differentiated in high and low density zones, b) In each case, its eytochemical characteristics are similar; highly positively to EDTA (preferential stain for RNP) being observed in the low density zones. As scattered chromatin these would constitute the active regions of the NOR. c) In certain eases a clear relationship is seen between NOR volmne and the size of the nucleolus, d) The chromatin associated to the NOR always appears as very dense zones, e) The NOR-Nucleolus relationships are established exclusively with the latter's fibrillar pars. f) The nucleolar segregation phenomenon takes place some- times as a result of a NOR contraction and in other cases depends on the position of the NOR in relation to the nucleolus, g) In preleptotene, the NOR shows its characteristic morphology and is positive to EDTA, while from zygotene to diplotene the structure of the NOR presents no differences from the rest of the ehromatin and only the lateral elements of the synaptonemal complex or its remnants are positive to EDTA. h) The symaptonemal complex runs through the NOt~ with no differentiation and on occasions its lateral elements show associations with the nueleolus." @default.
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- W2576446190 date "1975-01-01" @default.
- W2576446190 modified "2023-09-26" @default.
- W2576446190 title "Nucleolar Organizer Uhrastructure in Allium cepa" @default.
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