FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2577431549> ?p ?o ?g. }

• W2577431549 abstract "Background: Pumilio/fem-3 mRNA binding factor (PUF) proteins can bind RNA in a sequence-specific manner. The deciphered RNA-recognition code of these proteins has enabled researchers to design engineered PUF proteins, capable of binding to any desired target in order to modify its ultimate fate. In this study, a modified Homo sapiens Pumilio 1-homology domain (HsPUM1-HD) was engineered to bind to the internal ribosome entry site (IRES) of hepatitis C virus (HCV) genome to potentially inhibit viral translation. Methods: Based on the RNA-recognition code, required modifications were applied to HsPUM1-HD in order to change its natural recognition sequence to a sequence in the stem-loop III of HCV IRES. RNA protein pull-down assay was performed to assess the sequence specificity of the modified HsPUM1-HD (mHsPUM1-HD). Translational inhibitory effect of mHsPUM1-HD was evaluated in a dual-luciferase reporter assay. Results: The mHsPUM1-HD was found to bind to its cognate RNA in a sequence-specific manner, as a biotinylated target RNA captured mHsPUM1-HD through binding to streptavidin magnetic beads. This protein also reduced HCV IRES-dependent firefly luciferase translation by 40% in HEK293 cells. Conclusions: The present study is the first report of an engineered HsPUM1-HD with potential anti-HCV activity. These findings suggest that PUM-HDs can be engineered to target desired RNAs of infectious agents in order to specifically interrupt protein translation, as an essential step of their life cycle." @default.
• W2577431549 created "2017-01-26" @default.
• W2577431549 creator A5000702841 @default.
• W2577431549 creator A5027262289 @default.
• W2577431549 creator A5029184531 @default.
• W2577431549 creator A5029738861 @default.
• W2577431549 creator A5032672163 @default.
• W2577431549 creator A5045644761 @default.
• W2577431549 creator A5063036296 @default.
• W2577431549 creator A5073304331 @default.
• W2577431549 creator A5088377096 @default.
• W2577431549 date "2017-01-21" @default.
• W2577431549 modified "2023-09-26" @default.
• W2577431549 title "Repression of the Internal Ribosome Entry Site-dependent Translation of Hepatitis C Virus by an Engineered PUF Protein" @default.
• W2577431549 cites W1776345984 @default.
• W2577431549 cites W1964617068 @default.
• W2577431549 cites W1964658455 @default.
• W2577431549 cites W1967819482 @default.
• W2577431549 cites W1970339917 @default.
• W2577431549 cites W1971124068 @default.
• W2577431549 cites W1977300462 @default.
• W2577431549 cites W1983108685 @default.
• W2577431549 cites W1999269806 @default.
• W2577431549 cites W2000549137 @default.
• W2577431549 cites W2002239006 @default.
• W2577431549 cites W2017201840 @default.
• W2577431549 cites W2040017095 @default.
• W2577431549 cites W2046477882 @default.
• W2577431549 cites W2049003872 @default.
• W2577431549 cites W2060213717 @default.
• W2577431549 cites W2068068897 @default.
• W2577431549 cites W2077734499 @default.
• W2577431549 cites W2083381199 @default.
• W2577431549 cites W2090807582 @default.
• W2577431549 cites W2092244766 @default.
• W2577431549 cites W2140518449 @default.
• W2577431549 cites W2160390885 @default.
• W2577431549 cites W2169300762 @default.
• W2577431549 cites W2261411023 @default.
• W2577431549 cites W2311789374 @default.
• W2577431549 cites W2318156787 @default.
• W2577431549 doi "https://doi.org/10.5812/hepatmon.45022" @default.
• W2577431549 hasPublicationYear "2017" @default.
• W2577431549 type Work @default.
• W2577431549 sameAs 2577431549 @default.
• W2577431549 citedByCount "1" @default.
• W2577431549 countsByYear W25774315492021 @default.
• W2577431549 crossrefType "journal-article" @default.
• W2577431549 hasAuthorship W2577431549A5000702841 @default.
• W2577431549 hasAuthorship W2577431549A5027262289 @default.
• W2577431549 hasAuthorship W2577431549A5029184531 @default.
• W2577431549 hasAuthorship W2577431549A5029738861 @default.
• W2577431549 hasAuthorship W2577431549A5032672163 @default.
• W2577431549 hasAuthorship W2577431549A5045644761 @default.
• W2577431549 hasAuthorship W2577431549A5063036296 @default.
• W2577431549 hasAuthorship W2577431549A5073304331 @default.
• W2577431549 hasAuthorship W2577431549A5088377096 @default.
• W2577431549 hasConcept C104317684 @default.
• W2577431549 hasConcept C105580179 @default.
• W2577431549 hasConcept C111335760 @default.
• W2577431549 hasConcept C149364088 @default.
• W2577431549 hasConcept C153911025 @default.
• W2577431549 hasConcept C41282012 @default.
• W2577431549 hasConcept C54009773 @default.
• W2577431549 hasConcept C54355233 @default.
• W2577431549 hasConcept C67705224 @default.
• W2577431549 hasConcept C70721500 @default.
• W2577431549 hasConcept C77430603 @default.
• W2577431549 hasConcept C86803240 @default.
• W2577431549 hasConcept C88478588 @default.
• W2577431549 hasConcept C95444343 @default.
• W2577431549 hasConceptScore W2577431549C104317684 @default.
• W2577431549 hasConceptScore W2577431549C105580179 @default.
• W2577431549 hasConceptScore W2577431549C111335760 @default.
• W2577431549 hasConceptScore W2577431549C149364088 @default.
• W2577431549 hasConceptScore W2577431549C153911025 @default.
• W2577431549 hasConceptScore W2577431549C41282012 @default.
• W2577431549 hasConceptScore W2577431549C54009773 @default.
• W2577431549 hasConceptScore W2577431549C54355233 @default.
• W2577431549 hasConceptScore W2577431549C67705224 @default.
• W2577431549 hasConceptScore W2577431549C70721500 @default.
• W2577431549 hasConceptScore W2577431549C77430603 @default.
• W2577431549 hasConceptScore W2577431549C86803240 @default.
• W2577431549 hasConceptScore W2577431549C88478588 @default.
• W2577431549 hasConceptScore W2577431549C95444343 @default.
• W2577431549 hasIssue "2" @default.
• W2577431549 hasLocation W25774315491 @default.
• W2577431549 hasOpenAccess W2577431549 @default.
• W2577431549 hasPrimaryLocation W25774315491 @default.
• W2577431549 hasRelatedWork W1994855785 @default.
• W2577431549 hasRelatedWork W1995269618 @default.
• W2577431549 hasRelatedWork W2063058717 @default.
• W2577431549 hasRelatedWork W2114859361 @default.
• W2577431549 hasRelatedWork W2419044071 @default.
• W2577431549 hasRelatedWork W2589945527 @default.
• W2577431549 hasRelatedWork W2913827334 @default.
• W2577431549 hasRelatedWork W2999223988 @default.
• W2577431549 hasRelatedWork W4241125077 @default.
• W2577431549 hasRelatedWork W4288858747 @default.
• W2577431549 hasVolume "17" @default.

• next