Matches in SemOpenAlex for { <https://semopenalex.org/work/W2577928160> ?p ?o ?g. }
- W2577928160 abstract "Mitochondrial fusion is thought to be important for supporting cardiac contractility, but is hardly detectable in cultured cardiomyocytes and is difficult to directly evaluate in the heart. We overcame this obstacle through in vivo adenoviral transduction with matrix-targeted photoactivatable GFP and confocal microscopy. Imaging in whole rat hearts indicated mitochondrial network formation and fusion activity in ventricular cardiomyocytes. Promptly after isolation, cardiomyocytes showed extensive mitochondrial connectivity and fusion, which decayed in culture (at 24-48 h). Fusion manifested both as rapid content mixing events between adjacent organelles and slower events between both neighboring and distant mitochondria. Loss of fusion in culture likely results from the decline in calcium oscillations/contractile activity and mitofusin 1 (Mfn1), because (i) verapamil suppressed both contraction and mitochondrial fusion, (ii) after spontaneous contraction or short-term field stimulation fusion activity increased in cardiomyocytes, and (iii) ryanodine receptor-2-mediated calcium oscillations increased fusion activity in HEK293 cells and complementing changes occurred in Mfn1. Weakened cardiac contractility in vivo in alcoholic animals is also associated with depressed mitochondrial fusion. Thus, attenuated mitochondrial fusion might contribute to the pathogenesis of cardiomyopathy." @default.
- W2577928160 created "2017-01-26" @default.
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- W2577928160 date "2017-01-17" @default.
- W2577928160 modified "2023-10-15" @default.
- W2577928160 title "Mitochondrial fusion dynamics is robust in the heart and depends on calcium oscillations and contractile activity" @default.
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- W2577928160 cites W1570973990 @default.
- W2577928160 cites W1650401181 @default.
- W2577928160 cites W1766184155 @default.
- W2577928160 cites W1940751968 @default.
- W2577928160 cites W1964317311 @default.
- W2577928160 cites W1965511073 @default.
- W2577928160 cites W1971978398 @default.
- W2577928160 cites W1983710068 @default.
- W2577928160 cites W1987938334 @default.
- W2577928160 cites W1990171977 @default.
- W2577928160 cites W1991994507 @default.
- W2577928160 cites W1999753655 @default.
- W2577928160 cites W2003046582 @default.
- W2577928160 cites W2004550726 @default.
- W2577928160 cites W2004620514 @default.
- W2577928160 cites W2005150915 @default.
- W2577928160 cites W2006899261 @default.
- W2577928160 cites W2007546912 @default.
- W2577928160 cites W2008888835 @default.
- W2577928160 cites W2016971316 @default.
- W2577928160 cites W2023283007 @default.
- W2577928160 cites W2028452724 @default.
- W2577928160 cites W2028620319 @default.
- W2577928160 cites W2029204107 @default.
- W2577928160 cites W2037192206 @default.
- W2577928160 cites W2039440815 @default.
- W2577928160 cites W2043346651 @default.
- W2577928160 cites W2049312885 @default.
- W2577928160 cites W2059065327 @default.
- W2577928160 cites W2059320820 @default.
- W2577928160 cites W2064570455 @default.
- W2577928160 cites W2069730374 @default.
- W2577928160 cites W2071906706 @default.
- W2577928160 cites W2081342132 @default.
- W2577928160 cites W2083155168 @default.
- W2577928160 cites W2083502135 @default.
- W2577928160 cites W2085367705 @default.
- W2577928160 cites W2086557228 @default.
- W2577928160 cites W2093504307 @default.
- W2577928160 cites W2095652212 @default.
- W2577928160 cites W2097049860 @default.
- W2577928160 cites W2101257295 @default.
- W2577928160 cites W2110260487 @default.
- W2577928160 cites W2115653374 @default.
- W2577928160 cites W2119900852 @default.
- W2577928160 cites W2126133274 @default.
- W2577928160 cites W2127775546 @default.
- W2577928160 cites W2128600840 @default.
- W2577928160 cites W2130025116 @default.
- W2577928160 cites W2130758099 @default.
- W2577928160 cites W2133232243 @default.
- W2577928160 cites W2135314348 @default.
- W2577928160 cites W2135645000 @default.
- W2577928160 cites W2140559391 @default.
- W2577928160 cites W2140975536 @default.
- W2577928160 cites W2142192946 @default.
- W2577928160 cites W2151698573 @default.
- W2577928160 cites W2152527646 @default.
- W2577928160 cites W2152674951 @default.
- W2577928160 cites W2158725951 @default.
- W2577928160 cites W2159453909 @default.
- W2577928160 cites W2160660790 @default.
- W2577928160 cites W2190642228 @default.
- W2577928160 cites W2229506676 @default.
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- W2577928160 doi "https://doi.org/10.1073/pnas.1617288114" @default.
- W2577928160 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5293028" @default.
- W2577928160 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28096338" @default.
- W2577928160 hasPublicationYear "2017" @default.
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