FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2578169323> ?p ?o ?g. }

• W2578169323 endingPage "783" @default.
• W2578169323 startingPage "766" @default.
• W2578169323 abstract "The preparation of a glycosynthase, a catalytic nucleophile mutant of a glycosidase, is a well-established strategy for the effective synthesis of glycosidic linkages. However, glycosynthases derived from α-glycosidases can give poor yields of desired products because they require generally unstable β-glycosyl fluoride donors. Here, we investigate a transglycosylation catalyzed by a catalytic nucleophile mutant derived from a glycoside hydrolase family (GH) 97 α-galactosidase, using more stable β-galactosyl azide and α-galactosyl fluoride donors. The mutant enzyme catalyzes the glycosynthase reaction using β-galactosyl azide and α-galactosyl transfer from α-galactosyl fluoride with assistance of external anions. Formate was more effective at restoring transfer activity than azide. Kinetic analysis suggests that poor transglycosylation in the presence of the azide is because of low activity of the ternary complex between enzyme, β-galactosyl azide and acceptor. A three-dimensional structure of the mutant enzyme in complex with the transglycosylation product, β-lactosyl α-d-galactoside, was solved to elucidate the ligand-binding aspects of the α-galactosidase. Subtle differences at the β→α loops 1, 2 and 3 of the catalytic TIM barrel of the α-galactosidase from those of a homologous GH97 α-glucoside hydrolase seem to be involved in substrate recognitions. In particular, the Trp residues in β→α loop 1 have separate roles. Trp312 of the α-galactosidase appears to exclude the equatorial hydroxy group at C4 of glucosides, whereas the corresponding Trp residue in the α-glucoside hydrolase makes a hydrogen bond with this hydroxy group. The mechanism of α-galactoside recognition is conserved among GH27, 31, 36 and 97 α-galactosidases.The atomic coordinates (code: 5E1Q) have been deposited in the Protein Data Bank." @default.
• W2578169323 created "2017-01-26" @default.
• W2578169323 creator A5006117348 @default.
• W2578169323 creator A5009710587 @default.
• W2578169323 creator A5010559104 @default.
• W2578169323 creator A5016092223 @default.
• W2578169323 creator A5017022704 @default.
• W2578169323 creator A5022233637 @default.
• W2578169323 creator A5025387370 @default.
• W2578169323 creator A5058406239 @default.
• W2578169323 creator A5083249193 @default.
• W2578169323 creator A5086997944 @default.
• W2578169323 creator A5089759590 @default.
• W2578169323 date "2017-02-08" @default.
• W2578169323 modified "2023-10-06" @default.
• W2578169323 title "Efficient synthesis of α‐galactosyl oligosaccharides using a mutant <i>Bacteroides thetaiotaomicron</i> retaining α‐galactosidase ( <i>Bt</i> <scp>GH</scp> 97b)" @default.
• W2578169323 cites W1536977761 @default.
• W2578169323 cites W1965897156 @default.
• W2578169323 cites W1976261957 @default.
• W2578169323 cites W1976745215 @default.
• W2578169323 cites W1979566946 @default.
• W2578169323 cites W1997888072 @default.
• W2578169323 cites W2002818224 @default.
• W2578169323 cites W2014090403 @default.
• W2578169323 cites W2016784758 @default.
• W2578169323 cites W2030271524 @default.
• W2578169323 cites W2038423630 @default.
• W2578169323 cites W2039021127 @default.
• W2578169323 cites W2042112590 @default.
• W2578169323 cites W2044509450 @default.
• W2578169323 cites W2085340024 @default.
• W2578169323 cites W2087066120 @default.
• W2578169323 cites W2093995674 @default.
• W2578169323 cites W2124026197 @default.
• W2578169323 cites W2127753803 @default.
• W2578169323 cites W2154197653 @default.
• W2578169323 cites W2154714625 @default.
• W2578169323 cites W2163341755 @default.
• W2578169323 cites W2164885552 @default.
• W2578169323 cites W2180229411 @default.
• W2578169323 cites W2206172463 @default.
• W2578169323 cites W2950421900 @default.
• W2578169323 cites W4248872320 @default.
• W2578169323 doi "https://doi.org/10.1111/febs.14018" @default.
• W2578169323 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28103425" @default.
• W2578169323 hasPublicationYear "2017" @default.
• W2578169323 type Work @default.
• W2578169323 sameAs 2578169323 @default.
• W2578169323 citedByCount "18" @default.
• W2578169323 countsByYear W25781693232017 @default.
• W2578169323 countsByYear W25781693232018 @default.
• W2578169323 countsByYear W25781693232020 @default.
• W2578169323 countsByYear W25781693232021 @default.
• W2578169323 countsByYear W25781693232022 @default.
• W2578169323 countsByYear W25781693232023 @default.
• W2578169323 crossrefType "journal-article" @default.
• W2578169323 hasAuthorship W2578169323A5006117348 @default.
• W2578169323 hasAuthorship W2578169323A5009710587 @default.
• W2578169323 hasAuthorship W2578169323A5010559104 @default.
• W2578169323 hasAuthorship W2578169323A5016092223 @default.
• W2578169323 hasAuthorship W2578169323A5017022704 @default.
• W2578169323 hasAuthorship W2578169323A5022233637 @default.
• W2578169323 hasAuthorship W2578169323A5025387370 @default.
• W2578169323 hasAuthorship W2578169323A5058406239 @default.
• W2578169323 hasAuthorship W2578169323A5083249193 @default.
• W2578169323 hasAuthorship W2578169323A5086997944 @default.
• W2578169323 hasAuthorship W2578169323A5089759590 @default.
• W2578169323 hasBestOaLocation W25781693231 @default.
• W2578169323 hasConcept C104317684 @default.
• W2578169323 hasConcept C143065580 @default.
• W2578169323 hasConcept C150487720 @default.
• W2578169323 hasConcept C178790620 @default.
• W2578169323 hasConcept C181199279 @default.
• W2578169323 hasConcept C185592680 @default.
• W2578169323 hasConcept C196032511 @default.
• W2578169323 hasConcept C2776392439 @default.
• W2578169323 hasConcept C2778168646 @default.
• W2578169323 hasConcept C2780120296 @default.
• W2578169323 hasConcept C2780906977 @default.
• W2578169323 hasConcept C2780997848 @default.
• W2578169323 hasConcept C2908934157 @default.
• W2578169323 hasConcept C43053761 @default.
• W2578169323 hasConcept C523546767 @default.
• W2578169323 hasConcept C54355233 @default.
• W2578169323 hasConcept C547475151 @default.
• W2578169323 hasConcept C55493867 @default.
• W2578169323 hasConcept C71240020 @default.
• W2578169323 hasConcept C86803240 @default.
• W2578169323 hasConceptScore W2578169323C104317684 @default.
• W2578169323 hasConceptScore W2578169323C143065580 @default.
• W2578169323 hasConceptScore W2578169323C150487720 @default.
• W2578169323 hasConceptScore W2578169323C178790620 @default.
• W2578169323 hasConceptScore W2578169323C181199279 @default.
• W2578169323 hasConceptScore W2578169323C185592680 @default.
• W2578169323 hasConceptScore W2578169323C196032511 @default.
• W2578169323 hasConceptScore W2578169323C2776392439 @default.
• W2578169323 hasConceptScore W2578169323C2778168646 @default.
• W2578169323 hasConceptScore W2578169323C2780120296 @default.

• next