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- W2578422622 abstract "Abstract Pilidiella granati , a causal agent of twig blight and crown rot of pomegranate, is an emerging threat that may cause severe risk to the pomegranate industry in the future. Development of a rapid assay for the timely and accurate detection of P. granati will be helpful in the active surveillance and management of the disease caused by this pathogen. In this study, a nested PCR method was established for the detection of P. granati . Comparative analysis of genetic diversity within 5.8S rDNA internal transcribed spacer (ITS) sequences of P. granati and 21 other selected fungal species was performed to design species-specific primers (S1 and S2). This primer pair successfully amplified a 450 bp product exclusively from the genomic DNA of P. granati . The developed method can detect 10 pg genomic DNA of the pathogen in about 6 h. This technique was successfully applied to detect the natural infection of P. granati in the pomegranate fruit. The designed protocol is rapid and precise with a high degree of sensitivity." @default.
- W2578422622 created "2017-01-26" @default.
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- W2578422622 date "2017-01-20" @default.
- W2578422622 modified "2023-10-17" @default.
- W2578422622 title "Development of a nested-PCR assay for the rapid detection of Pilidiella granati in pomegranate fruit" @default.
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- W2578422622 doi "https://doi.org/10.1038/srep40954" @default.
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