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• W2578444530 abstract "GFP-binding protein (or GBP) has been recently developed in various systems and organisms as an efficient tool to purify GFP-fusion proteins. Due to the high affinity between GBP and GFP or GFP variants, this GBP-based approach is also ideally suited to alter the localization of functional proteins in live cells. In order to facilitate the wide use of the GBP-targeting approach in the fission yeast Schizosaccharomyces pombe, we developed a set of pFA6a-, pJK148- and pUC119-based vectors containing GBP- or GBP-mCherry-coding sequences and variants of inducible nmt1 or constitutive adh1 promoters that result in different levels of expression. The GBP or GBP-mCherry fragments can serve as cassettes for N- or C-terminal genomic tagging of genes of interest. We illustrated the application of these vectors in the construction of yeast strains with Dma1 or Cdc7 tagged with GBP-mCherry and efficient targeting of Dma1- or Cdc7-GBP-mCherry to the spindle pole body by Sid4-GFP. This series of vectors should help to facilitate the application of the GBP-targeting approach in manipulating protein localization and the analysis of gene function in fission yeast, at the level of single genes, as well as at a systematic scale." @default.
• W2578444530 created "2017-01-26" @default.
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• W2578444530 date "2017-01-01" @default.
• W2578444530 modified "2023-10-15" @default.
• W2578444530 title "Facile manipulation of protein localization in fission yeast through GBP-GFP binding" @default.
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• W2578444530 doi "https://doi.org/10.1242/jcs.198457" @default.
• W2578444530 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28082423" @default.
• W2578444530 hasPublicationYear "2017" @default.
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