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- W2578542931 abstract "The role of ATP-sulphurylases in the enzymology of accelerated primary sludge solubilisation utilising sulphate reducing systems was investigated. ATP-sulphurylase levels were monitored in methanogenic (ATPS MR ) and sulphidogenic (ATPS SR ) bioreactor systems. ATPS MR and ATPS SR activities were mainly present in the cell free fractions of the bioreactor sludge. ATPS MR activity was significantly lower (10–20-fold) than the activity observed for ATPS SR . Studies of pH optimisation showed a pH optima of 9.0 for ATPS SR . ATPS MR and ATPS SR exhibited temperature optima of 50 and 55 °C respectively, with ATPS SR showing a higher degree of temperature stability at its temperature optimum. Sulphate and sulphide had no effect on ATPS activity. The effect of volatile acids, lime (CaO) and divalent metal ions on ATPS activity was also investigated. Mg 2+ ions were required for activity. ATPS SR activity rapidly increased in the closed system sulphidogenic reactor over the first 3–5 days, coincident with sulphate concentration decreases. In conclusion, ATPS activity therefore appears to be a possible marker of sulphate reduction and anaerobic digestion of sludge using a sulphidogenic bioreactor system. Much more research, however, needs to be performed in order to verify a definite and quantitative relationship between ATPS activity and the presence of an active SRB population." @default.
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- W2578542931 date "2002-08-01" @default.
- W2578542931 modified "2023-10-14" @default.
- W2578542931 title "The enzymology of sludge solubilisation utilising sulphate reducing systems" @default.
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- W2578542931 doi "https://doi.org/10.1016/s0141-0229(02)00126-6" @default.
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