FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2578837604> ?p ?o ?g. }

• W2578837604 abstract "ABSTRACT Denitrifying Epsilonproteobacteria may dominate nitrogen loss processes in marine habitats with intense redox gradients, but assessment of their importance is limited by the currently available primers for nitrite reductase genes. Nine new primers targeting the nirS gene of denitrifying Epsilonproteobacteria were designed and tested for use in sequencing and quantitative PCR on two microbial mat samples (vent 2 and vent 4) from the Calypso hydrothermal vent field, Bay of Plenty, New Zealand. Commonly used nirS and nirK primer sets nirS1F/nirS6R, cd3aF/R3cd, nirK1F/nirK5R, and F1aCu/R3Cu were also tested to determine what may be missed by the common single-primer approach to assessing denitrifier diversity. The relative importance of Epsilonproteobacteria in these samples was evaluated by 16S rRNA gene sequencing. Epsilonproteobacteria represented up to 75.6% of 16S rRNA libraries, but nirS genes from this group were not found with commonly used primers. Pairing of the new primer EPSnirS511F with either EPSnirS1100R or EPSnirS1105R recovered nirS sequences from members of the genera Sulfurimonas , Sulfurovum , and Nitratifractor. The new quantitative PCR primers EPSnirS103F/EPSnirS530R showed dominance of denitrifying Epsilonproteobacteria in vent 4 compared to vent 2, which had greater representation by “standard” denitrifiers measured with the cd3aF/R3cd primers. Limited results from commonly used nirK primers suggest biased amplification between primers. Future application of multiple nirS and nirK primers, including the new epsilonproteobacterial nirS primers, will improve the detection of denitrifier diversity and the capability to identify changes in dominant denitrifying communities. IMPORTANCE Estimating the potential for increasing nitrogen limitation in the changing global ocean is reliant on understanding the microbial community that removes nitrogen through the process of denitrification. This process is favored under oxygen limitation, which is a growing global-ocean phenomenon. Current methods use the nitrite reductase genes nirS and nirK to assess denitrifier diversity and abundance using primers that target only a few known denitrifiers and systematically exclude denitrifying Epsilonproteobacteria , a group known to dominate in reducing environments, such as hydrothermal vents and anoxic basins. As oxygen depletion expands in the oceans, it is important to study denitrifier community dynamics within those areas to predict future global ocean changes. This study explores the design and testing of new primers that target epsilonproteobacterial nirS and reveals the varied success of existing primers, leading to the recommendation of a multiple-primer approach to assessing denitrifier diversity." @default.
• W2578837604 created "2017-01-26" @default.
• W2578837604 creator A5023338243 @default.
• W2578837604 creator A5024823372 @default.
• W2578837604 date "2017-03-15" @default.
• W2578837604 modified "2023-10-12" @default.
• W2578837604 title "Capturing Compositional Variation in Denitrifying Communities: a Multiple-Primer Approach That Includes Epsilonproteobacteria" @default.
• W2578837604 cites W1484969131 @default.
• W2578837604 cites W1534233155 @default.
• W2578837604 cites W1606773497 @default.
• W2578837604 cites W1623583506 @default.
• W2578837604 cites W1805480619 @default.
• W2578837604 cites W1842957400 @default.
• W2578837604 cites W1927876871 @default.
• W2578837604 cites W1954443682 @default.
• W2578837604 cites W1964292554 @default.
• W2578837604 cites W1966461026 @default.
• W2578837604 cites W1973727182 @default.
• W2578837604 cites W1983374387 @default.
• W2578837604 cites W1983447033 @default.
• W2578837604 cites W1991521911 @default.
• W2578837604 cites W1991536702 @default.
• W2578837604 cites W2010697718 @default.
• W2578837604 cites W2015494293 @default.
• W2578837604 cites W2018472255 @default.
• W2578837604 cites W2021641118 @default.
• W2578837604 cites W2022019284 @default.
• W2578837604 cites W2025018339 @default.
• W2578837604 cites W2028732139 @default.
• W2578837604 cites W2029039279 @default.
• W2578837604 cites W2033972004 @default.
• W2578837604 cites W2035055175 @default.
• W2578837604 cites W2035245651 @default.
• W2578837604 cites W2036677156 @default.
• W2578837604 cites W2037065956 @default.
• W2578837604 cites W2046953740 @default.
• W2578837604 cites W2054385769 @default.
• W2578837604 cites W2055595739 @default.
• W2578837604 cites W2062941070 @default.
• W2578837604 cites W2063482179 @default.
• W2578837604 cites W2063881343 @default.
• W2578837604 cites W2070685168 @default.
• W2578837604 cites W2074668828 @default.
• W2578837604 cites W2082092506 @default.
• W2578837604 cites W2086131703 @default.
• W2578837604 cites W2086903981 @default.
• W2578837604 cites W2096710218 @default.
• W2578837604 cites W2098732866 @default.
• W2578837604 cites W2101744484 @default.
• W2578837604 cites W2102246838 @default.
• W2578837604 cites W2102555068 @default.
• W2578837604 cites W2104331814 @default.
• W2578837604 cites W2105405291 @default.
• W2578837604 cites W2107459786 @default.
• W2578837604 cites W2108718991 @default.
• W2578837604 cites W2113517675 @default.
• W2578837604 cites W2113708808 @default.
• W2578837604 cites W2115090588 @default.
• W2578837604 cites W2117607560 @default.
• W2578837604 cites W2125080673 @default.
• W2578837604 cites W2125862549 @default.
• W2578837604 cites W2126787135 @default.
• W2578837604 cites W2126973059 @default.
• W2578837604 cites W2130077037 @default.
• W2578837604 cites W2130760715 @default.
• W2578837604 cites W2133231749 @default.
• W2578837604 cites W2136872570 @default.
• W2578837604 cites W2139989406 @default.
• W2578837604 cites W2143535440 @default.
• W2578837604 cites W2144654387 @default.
• W2578837604 cites W2144795626 @default.
• W2578837604 cites W2146931904 @default.
• W2578837604 cites W2147513370 @default.
• W2578837604 cites W2154206419 @default.
• W2578837604 cites W2155806125 @default.
• W2578837604 cites W2156078950 @default.
• W2578837604 cites W2157032655 @default.
• W2578837604 cites W2157584041 @default.
• W2578837604 cites W2162315106 @default.
• W2578837604 cites W2164404906 @default.
• W2578837604 cites W2164980838 @default.
• W2578837604 cites W2169779547 @default.
• W2578837604 cites W2288993775 @default.
• W2578837604 cites W4321428016 @default.
• W2578837604 doi "https://doi.org/10.1128/aem.02753-16" @default.
• W2578837604 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5335531" @default.
• W2578837604 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28087525" @default.
• W2578837604 hasPublicationYear "2017" @default.
• W2578837604 type Work @default.
• W2578837604 sameAs 2578837604 @default.
• W2578837604 citedByCount "8" @default.
• W2578837604 countsByYear W25788376042017 @default.
• W2578837604 countsByYear W25788376042018 @default.
• W2578837604 countsByYear W25788376042019 @default.
• W2578837604 countsByYear W25788376042020 @default.
• W2578837604 countsByYear W25788376042021 @default.
• W2578837604 countsByYear W25788376042023 @default.
• W2578837604 crossrefType "journal-article" @default.
• W2578837604 hasAuthorship W2578837604A5023338243 @default.
• W2578837604 hasAuthorship W2578837604A5024823372 @default.

• next