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- W257909211 abstract "Attempts using in vitro and in vivo selection procedures have been made to search for hammerhead ribozymes that have higher activities than the wild-type ribozyme and also to determine whether other sequences might be possible in the catalytic core of the hammerhead ribozyme. Active sequences selected in the past conformed broadly to the consensus core sequence except at A9, and no sequences were associated with higher activity than that of the hammerhead with the consensus core, an indication that the consensus sequence derived from viruses and virusoids is probably the optimal sequence [Vaish et al. (1997) Biochemistry 36, 6495-6501]. Recently, during construction of ribozyme expression vectors, we isolated a mutant hammerhead ribozyme, with an insertion of G between A9 and 610.1, that appeared to show significant activity [Kawasaki et at (1996) Nucleic Acids Res. 24, 3010-3016; Kawasaki et al. (1998) Nature 393, 284-289]. We, therefore, characterized kinetic properties of the G-inserted mutant ribozymes in terms of the NUX rule. We demonstrate that the NUX rule is basically applicable to the G-inserted ribozymes and, more importantly, one type of G-inserted ribozyme was very active with k_(cat), value of 6.4 min^(-1) in 50 mM Tris-HCl (pH 8.0) and 10 m1VI MgCl₂ at 37℃." @default.
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- W257909211 date "2001-01-01" @default.
- W257909211 modified "2023-09-22" @default.
- W257909211 title "Characterization in Terms of the NUX Rule of G - inserted Mutant Hammerhead Ribozymes with High Level of Catalytic Power" @default.
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