FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2579969810> ?p ?o ?g. }

• W2579969810 endingPage "311" @default.
• W2579969810 startingPage "302" @default.
• W2579969810 abstract "Adequate cleavage of von Willebrand factor (VWF) prevents formation of thrombi. ADAMTS13 is the main VWF-cleaving protease and its deficiency results in development of thrombotic microangiopathy. Besides ADAMTS13 other proteases may also possess VWF-cleaving activity, but their physiological importance in preventing thrombus formation is unknown. This study investigated if, and which, proteases could cleave VWF in the glomerulus. The content of the glomerular basement membrane (GBM) was studied as a reflection of processes occurring in the subendothelial glomerular space. VWF was incubated with human GBMs and VWF cleavage was assessed by multimer structure analysis, immunoblotting and mass spectrometry. VWF was cleaved into the smallest multimers by the GBM, which contained ADAMTS13 as well as neutrophil proteases, elastase, proteinase 3 (PR3), cathepsin-G and matrix-metalloproteinase 9. The most potent components of the GBM capable of VWF cleavage were in the serine protease or metalloprotease category, but not ADAMTS13. Neutralization of neutrophil serine proteases inhibited GBM-mediated VWF-cleaving activity, demonstrating a marked contribution of elastase and/or PR3. VWF-platelet strings formed on the surface of primary glomerular endothelial cells, in a perfusion system, were cleaved by both elastase and the GBM, a process blocked by elastase inhibitor. Ultramorphological studies of the human kidney demonstrated neutrophils releasing elastase into the GBM. Neutrophil proteases may contribute to VWF cleavage within the subendothelium, adjacent to the GBM, and thus regulate thrombus size. This anti-thrombotic mechanism would protect the normal kidney during inflammation and could also explain why most patients with ADAMTS13 deficiency do not develop severe kidney failure." @default.
• W2579969810 created "2017-02-03" @default.
• W2579969810 creator A5045374933 @default.
• W2579969810 creator A5051065759 @default.
• W2579969810 creator A5060743939 @default.
• W2579969810 creator A5061182560 @default.
• W2579969810 creator A5063510579 @default.
• W2579969810 creator A5069641539 @default.
• W2579969810 creator A5079696999 @default.
• W2579969810 date "2017-02-01" @default.
• W2579969810 modified "2023-10-16" @default.
• W2579969810 title "Neutrophil Protease Cleavage of Von Willebrand Factor in Glomeruli – An Anti-thrombotic Mechanism in the Kidney" @default.
• W2579969810 cites W1927547718 @default.
• W2579969810 cites W1976353181 @default.
• W2579969810 cites W1985604220 @default.
• W2579969810 cites W1989495194 @default.
• W2579969810 cites W1990309661 @default.
• W2579969810 cites W1992746467 @default.
• W2579969810 cites W1995712642 @default.
• W2579969810 cites W1997662874 @default.
• W2579969810 cites W2003057187 @default.
• W2579969810 cites W2006554530 @default.
• W2579969810 cites W2007892582 @default.
• W2579969810 cites W2012039176 @default.
• W2579969810 cites W2014955040 @default.
• W2579969810 cites W2015900829 @default.
• W2579969810 cites W2016223225 @default.
• W2579969810 cites W2016494863 @default.
• W2579969810 cites W2022496252 @default.
• W2579969810 cites W2026172668 @default.
• W2579969810 cites W2030337128 @default.
• W2579969810 cites W2032235878 @default.
• W2579969810 cites W2044505929 @default.
• W2579969810 cites W2058677502 @default.
• W2579969810 cites W2061649065 @default.
• W2579969810 cites W2062522227 @default.
• W2579969810 cites W2069739255 @default.
• W2579969810 cites W2071667691 @default.
• W2579969810 cites W2088850758 @default.
• W2579969810 cites W2089547663 @default.
• W2579969810 cites W2114604545 @default.
• W2579969810 cites W2115488282 @default.
• W2579969810 cites W2126021594 @default.
• W2579969810 cites W2128545033 @default.
• W2579969810 cites W2128747139 @default.
• W2579969810 cites W2130694311 @default.
• W2579969810 cites W2130918837 @default.
• W2579969810 cites W2134820637 @default.
• W2579969810 cites W2135960771 @default.
• W2579969810 cites W2139853313 @default.
• W2579969810 cites W2158423845 @default.
• W2579969810 cites W2177216368 @default.
• W2579969810 cites W2230724256 @default.
• W2579969810 cites W2273025879 @default.
• W2579969810 cites W2315229770 @default.
• W2579969810 cites W2353282499 @default.
• W2579969810 cites W2396818208 @default.
• W2579969810 cites W276645119 @default.
• W2579969810 cites W4235872675 @default.
• W2579969810 doi "https://doi.org/10.1016/j.ebiom.2017.01.032" @default.
• W2579969810 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5474509" @default.
• W2579969810 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28139439" @default.
• W2579969810 hasPublicationYear "2017" @default.
• W2579969810 type Work @default.
• W2579969810 sameAs 2579969810 @default.
• W2579969810 citedByCount "2" @default.
• W2579969810 countsByYear W25799698102020 @default.
• W2579969810 countsByYear W25799698102022 @default.
• W2579969810 crossrefType "journal-article" @default.
• W2579969810 hasAuthorship W2579969810A5045374933 @default.
• W2579969810 hasAuthorship W2579969810A5051065759 @default.
• W2579969810 hasAuthorship W2579969810A5060743939 @default.
• W2579969810 hasAuthorship W2579969810A5061182560 @default.
• W2579969810 hasAuthorship W2579969810A5063510579 @default.
• W2579969810 hasAuthorship W2579969810A5069641539 @default.
• W2579969810 hasAuthorship W2579969810A5079696999 @default.
• W2579969810 hasBestOaLocation W25799698101 @default.
• W2579969810 hasConcept C109523444 @default.
• W2579969810 hasConcept C126322002 @default.
• W2579969810 hasConcept C153911025 @default.
• W2579969810 hasConcept C167814343 @default.
• W2579969810 hasConcept C181199279 @default.
• W2579969810 hasConcept C182220744 @default.
• W2579969810 hasConcept C185592680 @default.
• W2579969810 hasConcept C203014093 @default.
• W2579969810 hasConcept C2776317360 @default.
• W2579969810 hasConcept C2776714187 @default.
• W2579969810 hasConcept C2776914184 @default.
• W2579969810 hasConcept C2777132095 @default.
• W2579969810 hasConcept C2777807008 @default.
• W2579969810 hasConcept C2777878052 @default.
• W2579969810 hasConcept C2779134260 @default.
• W2579969810 hasConcept C2779394231 @default.
• W2579969810 hasConcept C2780921031 @default.
• W2579969810 hasConcept C2781044401 @default.
• W2579969810 hasConcept C2781071845 @default.
• W2579969810 hasConcept C55493867 @default.
• W2579969810 hasConcept C55728118 @default.

• next