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- W2579975331 abstract "A flow cytometry-based assay was developed to assess the infective titer of two recombinant viruses: a recombinant herpes simplex type 2 (rHSV-2) and a recombinant canary pox (rALVAC.gfp). This method uses granularity of infected Vero and QT-35 cells, respectively, and correlates this to the infectious titer of virus samples. The percent of the cell populations with a high level of granularity could accurately be correlated to viral titers obtained through a traditional plaque assay, with R2 values greater than 0.8 using a semi-logarithmic scale. This approach offers a rapid, high-throughput method for infectious virus titration with similar accuracy to a traditional plaque assay." @default.
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- W2579975331 date "2019-11-01" @default.
- W2579975331 modified "2023-10-17" @default.
- W2579975331 title "A flow cytometric granularity assay for the quantification of infectious virus" @default.
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- W2579975331 doi "https://doi.org/10.1016/j.vaccine.2019.02.059" @default.
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