Matches in SemOpenAlex for { <https://semopenalex.org/work/W2580159006> ?p ?o ?g. }
- W2580159006 endingPage "e0170213" @default.
- W2580159006 startingPage "e0170213" @default.
- W2580159006 abstract "Pancreatic β-cells synthesize and release serotonin (5 hydroxytryptamine, 5HT); however, the role of 5HT receptors on glucose stimulated insulin secretion (GSIS) and the mechanisms mediating this function is not fully understood. The aims of this study were to determine the expression profile of 5HT receptors in murine MIN6 β-cells and to examine the effects of pharmacological activation of 5HT receptor Htr2b on GSIS and mitochondrial function.mRNA levels of 5HT receptors in MIN6 cells were quantified by RT qPCR. GSIS was assessed in MIN6 cells in response to global serotonergic activation with 5HT and pharmacological Htr2b activation or inhibition with BW723C86 or SB204741, respectively. In response to Htr2b activation also was evaluated the mRNA and protein levels of PGC1α and PPARy by RT-qPCR and western blotting and mitochondrial function by oxygen consumption rate (OCR) and ATP cellular content.We found that mRNA levels of most 5HT receptors were either very low or undetectable in MIN6 cells. By contrast, Htr2b mRNA was present at moderate levels in these cells. Preincubation (6 h) of MIN6 cells with 5HT or BW723C86 reduced GSIS and the effect of 5HT was prevented by SB204741. Preincubation with BW723C86 increased PGC1α and PPARy mRNA and protein levels and decreased mitochondrial respiration and ATP content in MIN6 cells.Our results indicate that prolonged Htr2b activation in murine β-cells decreases glucose-stimulated insulin secretion and mitochondrial activity by mechanisms likely dependent on enhanced PGC1α/PPARy expression." @default.
- W2580159006 created "2017-02-03" @default.
- W2580159006 creator A5003660480 @default.
- W2580159006 creator A5018462821 @default.
- W2580159006 creator A5034778675 @default.
- W2580159006 creator A5036003428 @default.
- W2580159006 creator A5037347164 @default.
- W2580159006 creator A5038458296 @default.
- W2580159006 creator A5054135554 @default.
- W2580159006 creator A5058630661 @default.
- W2580159006 creator A5078493031 @default.
- W2580159006 creator A5079262385 @default.
- W2580159006 date "2017-01-27" @default.
- W2580159006 modified "2023-10-14" @default.
- W2580159006 title "Prolonged Activation of the Htr2b Serotonin Receptor Impairs Glucose Stimulated Insulin Secretion and Mitochondrial Function in MIN6 Cells" @default.
- W2580159006 cites W1562688366 @default.
- W2580159006 cites W1763330810 @default.
- W2580159006 cites W1932150911 @default.
- W2580159006 cites W1964739304 @default.
- W2580159006 cites W1969894996 @default.
- W2580159006 cites W1976268033 @default.
- W2580159006 cites W1981469121 @default.
- W2580159006 cites W1988276497 @default.
- W2580159006 cites W1997349856 @default.
- W2580159006 cites W1998807018 @default.
- W2580159006 cites W2008941652 @default.
- W2580159006 cites W2015115839 @default.
- W2580159006 cites W2024207068 @default.
- W2580159006 cites W2032352600 @default.
- W2580159006 cites W2034119841 @default.
- W2580159006 cites W2049305923 @default.
- W2580159006 cites W2051567027 @default.
- W2580159006 cites W2058608918 @default.
- W2580159006 cites W2060284723 @default.
- W2580159006 cites W2065017200 @default.
- W2580159006 cites W2065127510 @default.
- W2580159006 cites W2082645451 @default.
- W2580159006 cites W2087393295 @default.
- W2580159006 cites W2089826022 @default.
- W2580159006 cites W2104943928 @default.
- W2580159006 cites W2108400677 @default.
- W2580159006 cites W2113945466 @default.
- W2580159006 cites W2114627400 @default.
- W2580159006 cites W2119833136 @default.
- W2580159006 cites W2130358596 @default.
- W2580159006 cites W2134123563 @default.
- W2580159006 cites W2135190897 @default.
- W2580159006 cites W2136881812 @default.
- W2580159006 cites W2138071306 @default.
- W2580159006 cites W2141063928 @default.
- W2580159006 cites W2146667178 @default.
- W2580159006 cites W2153684035 @default.
- W2580159006 cites W2153853866 @default.
- W2580159006 cites W2161395021 @default.
- W2580159006 cites W2167192842 @default.
- W2580159006 cites W2167793478 @default.
- W2580159006 cites W2172008034 @default.
- W2580159006 cites W2230681148 @default.
- W2580159006 cites W230357065 @default.
- W2580159006 cites W2416538884 @default.
- W2580159006 cites W414797555 @default.
- W2580159006 doi "https://doi.org/10.1371/journal.pone.0170213" @default.
- W2580159006 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5271329" @default.
- W2580159006 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28129327" @default.
- W2580159006 hasPublicationYear "2017" @default.
- W2580159006 type Work @default.
- W2580159006 sameAs 2580159006 @default.
- W2580159006 citedByCount "22" @default.
- W2580159006 countsByYear W25801590062018 @default.
- W2580159006 countsByYear W25801590062019 @default.
- W2580159006 countsByYear W25801590062020 @default.
- W2580159006 countsByYear W25801590062021 @default.
- W2580159006 countsByYear W25801590062022 @default.
- W2580159006 countsByYear W25801590062023 @default.
- W2580159006 crossrefType "journal-article" @default.
- W2580159006 hasAuthorship W2580159006A5003660480 @default.
- W2580159006 hasAuthorship W2580159006A5018462821 @default.
- W2580159006 hasAuthorship W2580159006A5034778675 @default.
- W2580159006 hasAuthorship W2580159006A5036003428 @default.
- W2580159006 hasAuthorship W2580159006A5037347164 @default.
- W2580159006 hasAuthorship W2580159006A5038458296 @default.
- W2580159006 hasAuthorship W2580159006A5054135554 @default.
- W2580159006 hasAuthorship W2580159006A5058630661 @default.
- W2580159006 hasAuthorship W2580159006A5078493031 @default.
- W2580159006 hasAuthorship W2580159006A5079262385 @default.
- W2580159006 hasBestOaLocation W25801590061 @default.
- W2580159006 hasConcept C126322002 @default.
- W2580159006 hasConcept C134018914 @default.
- W2580159006 hasConcept C170493617 @default.
- W2580159006 hasConcept C2775864247 @default.
- W2580159006 hasConcept C28859421 @default.
- W2580159006 hasConcept C37000724 @default.
- W2580159006 hasConcept C49039625 @default.
- W2580159006 hasConcept C53910766 @default.
- W2580159006 hasConcept C71924100 @default.
- W2580159006 hasConcept C86803240 @default.
- W2580159006 hasConcept C95444343 @default.
- W2580159006 hasConceptScore W2580159006C126322002 @default.