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- W2580253375 abstract "The capability of multi-level logic operations in biological diagnosis is far from being fully realized. In particular, the simplification of logic judgments to realize rapid, on-site complex diagnoses is still a key issue in bioelectronics. The authors here describe the construction of a colorimetric multilevel DNA circuit that incorporates two IMPLY gates and a sample extraction technology (SET) with anti-BPA aptamer as a co-recognizing element and the color of gold nanoparticles as the output signal. The circuit can perform multi-bioanalysis of the plasticizers and hormone mimetics bisphenol A (BPA) and bisphenol S (BPS). The assay is based on the finding that the aptamer against BPA can recognize both BPA and BPS. This DNA logic detection system is simple, fast, sensitive and selective because of the utilization of a co-recognition element which decreased input numbers. This, in turn, cascades multiplex Boolean logic gates and simplifies the diagnostic challenge. Both BPA and BPS can be determined by photometry at 620 nm, the limit of detection being 1.5 ng⋅mL−1 for BPA and 1.3 ng⋅mL−1 for BPS. Application to (spiked) water samples shows good analytical performance in terms of recovery (95.4 to 106.8%), linear range (4.4 to 66 ng⋅mL−1 for BPA, 2.5 to 75 ng⋅mL−1 for BPS) and correlation coefficients (>0.98). In our perception, the method represents a simple route for the fabrication of colorimetric multi-level DNA circuits. It may shorten the gap between bioelectronic logic circuits and high throughput real sample analysis." @default.
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- W2580253375 date "2017-01-25" @default.
- W2580253375 modified "2023-09-25" @default.
- W2580253375 title "Simultaneous colorimetric determination of bisphenol A and bisphenol S via a multi-level DNA circuit mediated by aptamers and gold nanoparticles" @default.
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- W2580253375 doi "https://doi.org/10.1007/s00604-017-2092-8" @default.
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