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- W2580376921 startingPage "371" @default.
- W2580376921 abstract "Antimicrobial proteins and peptides (AMPs) are valuable as leads in the pharmaceutical industry for the development of novel anti-infective drugs. Here we describe the efficient heterologous expression and basic characterization of a Gloverin-family AMP derived from the greater wax moth Galleria mellonella. Highly productive single-cell clones prepared by limiting dilution achieved a 100% increase in productivity compared to the original polyclonal Drosophila melanogaster S2 cell line. Comprehensive screening for suitable expression conditions using statistical experimental designs revealed that optimal induction was achieved using 600 µM CuSO4 at the mid-exponential growth phase. Under these conditions, 25 mg/L of the AMP was expressed at the 1-L bioreactor scale, with optimal induction and harvest times ensured by dielectric spectroscopy and the online measurement of optical density. Gloverin was purified from the supernatant by immobilized metal ion affinity chromatography followed by dialysis. In growth assays, the purified protein showed specific antimicrobial activity against two different strains of Escherichia coli." @default.
- W2580376921 created "2017-02-03" @default.
- W2580376921 creator A5039582983 @default.
- W2580376921 creator A5067165691 @default.
- W2580376921 creator A5073456383 @default.
- W2580376921 date "2017-01-28" @default.
- W2580376921 modified "2023-09-25" @default.
- W2580376921 title "Optimized expression of the antimicrobial protein Gloverin from Galleria mellonella using stably transformed Drosophila melanogaster S2 cells" @default.
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- W2580376921 doi "https://doi.org/10.1007/s10616-017-0068-5" @default.