Matches in SemOpenAlex for { <https://semopenalex.org/work/W2580404925> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W2580404925 endingPage "219" @default.
- W2580404925 startingPage "215" @default.
- W2580404925 abstract "Background Research showed that transforming growth factor-β2 (TGF-β2) promotes scar formation.But its mechanism in scarring after glaucoma filtration surgery is worthy of studying.Objective This study was to investigate the effect of TGF-β2 on myofibroblast transition of human Tenon fibroblasts (HTFs) and scarring after glaucoma filtration surgery.Methods Tenon capsular tissue was obtained from 3 patients with strabismus during the surgery and was incubated in DMEM with 10％ fetal bovine serum (FBS).The cells were collected and passaged in the free-serum medium for 24 hours,and then 1,2,5,10,20 μg/L TGF-β2 was added into the medium respectively,to induce the transformation of HTFs,and 2 μg/L or 5 μg/L TGF-β2 was used to treat the HTFs for 6,24,48 and 72 hours.The control group was not treated with TGF-β2.The expressions of α-smooth muscle actin (α-SMA) and phosphorylation of the signaling proteins (pSmad2) in HTFs were detected by Western blot assay.The expressions of α-SMA and F-actin were located by cell immunofluorescine technique under the confocal immunofluorescence microscopy.Cell contractility was determined by collagen gel contraction assays.This study was approved by Ethic Committee of Institute of Surgery Research of Daping Hospital,and informed consent was obtained from each patient or custodian initial of the study.Results The expression of α-SMA protein in the HTFs was increased significantly after the treatment of TGF-β2 in comparison with the control group and reached a peak at 24-48 hours.The α-SMA expression was gradually weakened in the 10 μg/L TGF-β2 groups.Little of α-SMA and F-actin were expressed in the control group.However,strong staining for α-SMA and F-actin were observed in the 1,2 and 5 μg/L TGF-β2 groups and then the staining weakened at the concentration of 10 μg/L.In addition,pSmad2 showed a stronger expression in the 2 μg/L TGF-β2 group than that in the PBS group and FBS group,with the strongest expression in 30 minutes through 2 hours.The untreated gel contracted (78.00±3.13)％ from its initial size,and contraction in the 1,2,5,10 μg/L TGF-β2 group were (63.88±1.78)％,(20.69±0.65)％,(19.49-±0.54)％,(16.24±0.84) ％,respectively,TGF-β2 increased HTFs contraction significantly (Fgroup =859.400,P =0.000).Conclusions TGF-β2 can induce transdifferentiation of Tenon fibroblast into myofibroblast and increase cell contractility,with a concentration-dependent and time-dependent pattern to an extent.It may be the mechanism of scar formation after glaucoma filter surgery.Key words: Transforming growth factor-β2 ; Glaucoma/filtering surgery; Scar; Tenon fibroblast; α-Smooth muscle actin" @default.
- W2580404925 created "2017-02-03" @default.
- W2580404925 creator A5000319667 @default.
- W2580404925 creator A5027369632 @default.
- W2580404925 creator A5060683193 @default.
- W2580404925 creator A5065438419 @default.
- W2580404925 date "2013-03-10" @default.
- W2580404925 modified "2023-09-24" @default.
- W2580404925 title "Effects of transforming growth factor-β2 on human Tenon fibroblasts transformation and scarring after glaucoma filtration surgery" @default.
- W2580404925 doi "https://doi.org/10.3760/cma.j.issn.2095-0160.2013.03.002" @default.
- W2580404925 hasPublicationYear "2013" @default.
- W2580404925 type Work @default.
- W2580404925 sameAs 2580404925 @default.
- W2580404925 citedByCount "0" @default.
- W2580404925 crossrefType "journal-article" @default.
- W2580404925 hasAuthorship W2580404925A5000319667 @default.
- W2580404925 hasAuthorship W2580404925A5027369632 @default.
- W2580404925 hasAuthorship W2580404925A5060683193 @default.
- W2580404925 hasAuthorship W2580404925A5065438419 @default.
- W2580404925 hasConcept C104317684 @default.
- W2580404925 hasConcept C118131993 @default.
- W2580404925 hasConcept C118487528 @default.
- W2580404925 hasConcept C134018914 @default.
- W2580404925 hasConcept C141071460 @default.
- W2580404925 hasConcept C142724271 @default.
- W2580404925 hasConcept C1491633281 @default.
- W2580404925 hasConcept C16685009 @default.
- W2580404925 hasConcept C185592680 @default.
- W2580404925 hasConcept C207865475 @default.
- W2580404925 hasConcept C2776415932 @default.
- W2580404925 hasConcept C2779404806 @default.
- W2580404925 hasConcept C2780559512 @default.
- W2580404925 hasConcept C55493867 @default.
- W2580404925 hasConcept C71924100 @default.
- W2580404925 hasConceptScore W2580404925C104317684 @default.
- W2580404925 hasConceptScore W2580404925C118131993 @default.
- W2580404925 hasConceptScore W2580404925C118487528 @default.
- W2580404925 hasConceptScore W2580404925C134018914 @default.
- W2580404925 hasConceptScore W2580404925C141071460 @default.
- W2580404925 hasConceptScore W2580404925C142724271 @default.
- W2580404925 hasConceptScore W2580404925C1491633281 @default.
- W2580404925 hasConceptScore W2580404925C16685009 @default.
- W2580404925 hasConceptScore W2580404925C185592680 @default.
- W2580404925 hasConceptScore W2580404925C207865475 @default.
- W2580404925 hasConceptScore W2580404925C2776415932 @default.
- W2580404925 hasConceptScore W2580404925C2779404806 @default.
- W2580404925 hasConceptScore W2580404925C2780559512 @default.
- W2580404925 hasConceptScore W2580404925C55493867 @default.
- W2580404925 hasConceptScore W2580404925C71924100 @default.
- W2580404925 hasIssue "03" @default.
- W2580404925 hasLocation W25804049251 @default.
- W2580404925 hasOpenAccess W2580404925 @default.
- W2580404925 hasPrimaryLocation W25804049251 @default.
- W2580404925 hasRelatedWork W2044270182 @default.
- W2580404925 hasRelatedWork W2073935494 @default.
- W2580404925 hasRelatedWork W2141716515 @default.
- W2580404925 hasRelatedWork W2203398845 @default.
- W2580404925 hasRelatedWork W2348033235 @default.
- W2580404925 hasRelatedWork W2363340356 @default.
- W2580404925 hasRelatedWork W2367514784 @default.
- W2580404925 hasRelatedWork W2409026531 @default.
- W2580404925 hasRelatedWork W2417233255 @default.
- W2580404925 hasRelatedWork W2806051243 @default.
- W2580404925 hasRelatedWork W2892182476 @default.
- W2580404925 hasRelatedWork W2978313315 @default.
- W2580404925 hasRelatedWork W3028809786 @default.
- W2580404925 hasRelatedWork W3029509515 @default.
- W2580404925 hasRelatedWork W3030419011 @default.
- W2580404925 hasRelatedWork W3031525680 @default.
- W2580404925 hasRelatedWork W3032440348 @default.
- W2580404925 hasRelatedWork W3032644363 @default.
- W2580404925 hasRelatedWork W3087910349 @default.
- W2580404925 hasRelatedWork W3196778810 @default.
- W2580404925 hasVolume "31" @default.
- W2580404925 isParatext "false" @default.
- W2580404925 isRetracted "false" @default.
- W2580404925 magId "2580404925" @default.
- W2580404925 workType "article" @default.