FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2580758420> ?p ?o ?g. }

• W2580758420 abstract "Triptolide and celastrol, two principal bioactive compounds in Tripterygium wilfordii, are produced from geranylgeranyl diphosphate (GGPP) and farnesyl diphosphate ((E,E)-FPP) through terpenoid biosynthesis pathway. However, little is known about T. wilfordii terpene synthases which could competitively utilize GGPP and (E,E)-FPP as substrates, producing C15 and C20 tertiary alcohols. Here we firstly cloned the genes encoding nerolidol synthase (NES) and geranyllinalool synthases (GES1, GES2), which are responsible for the biosynthesis of (E)-nerolidol and (E,E)-geranyllinalool. In vitro characterization of recombinant TwNES and TwGES1 revealed both were functional enzymes that could catalyze the conversion of (E,E)-FPP and GGPP to (E)-nerolidol and (E,E)-geranyllinalool, which were consistent with the results of yeast fermentation. Biochemical characterization revealed TwNES and TwGES1 had strong dependency for Mg2+, Km and Kcat/Km values of TwNES for (E,E)-FPP were 12.700 μM and 0.029 s-1/μM, and TwGES1 for GGPP were 2.039 μM and 0.019 s-1/μM. Real-time PCR analysis showed the expression levels of NES and GES1 increased by several fold in the suspension cells treated with alamethicin, indicating TwNES and TwGES1 are likely to utilize GGPP and (E,E)-FPP to generate tertiary alcohols as precursor of plant volatiles, which play important roles in the ecological interactions between T. wilfordii and other organisms." @default.
• W2580758420 created "2017-02-03" @default.
• W2580758420 creator A5019190057 @default.
• W2580758420 creator A5030202287 @default.
• W2580758420 creator A5038399698 @default.
• W2580758420 creator A5047721072 @default.
• W2580758420 creator A5072035000 @default.
• W2580758420 creator A5081788625 @default.
• W2580758420 creator A5082816482 @default.
• W2580758420 creator A5089822225 @default.
• W2580758420 creator A5090642719 @default.
• W2580758420 date "2017-01-27" @default.
• W2580758420 modified "2023-09-25" @default.
• W2580758420 title "Functional characterization of NES and GES responsible for the biosynthesis of (E)-nerolidol and (E,E)-geranyllinalool in Tripterygium wilfordii" @default.
• W2580758420 cites W1208816652 @default.
• W2580758420 cites W1508698624 @default.
• W2580758420 cites W1562164295 @default.
• W2580758420 cites W1968915912 @default.
• W2580758420 cites W1973707092 @default.
• W2580758420 cites W1983444305 @default.
• W2580758420 cites W2011237756 @default.
• W2580758420 cites W2025767103 @default.
• W2580758420 cites W2050684487 @default.
• W2580758420 cites W2050859437 @default.
• W2580758420 cites W2061357971 @default.
• W2580758420 cites W2062181188 @default.
• W2580758420 cites W2080926431 @default.
• W2580758420 cites W2081184252 @default.
• W2580758420 cites W2083565509 @default.
• W2580758420 cites W2089601190 @default.
• W2580758420 cites W2091344286 @default.
• W2580758420 cites W2100215239 @default.
• W2580758420 cites W2100585048 @default.
• W2580758420 cites W2101509576 @default.
• W2580758420 cites W2107229549 @default.
• W2580758420 cites W2107277218 @default.
• W2580758420 cites W2113926663 @default.
• W2580758420 cites W2125304937 @default.
• W2580758420 cites W2128491496 @default.
• W2580758420 cites W2128635872 @default.
• W2580758420 cites W2132304174 @default.
• W2580758420 cites W2136157665 @default.
• W2580758420 cites W2136474969 @default.
• W2580758420 cites W2151869765 @default.
• W2580758420 cites W2157840703 @default.
• W2580758420 cites W2159181928 @default.
• W2580758420 cites W2162705593 @default.
• W2580758420 cites W2165023533 @default.
• W2580758420 cites W2168920342 @default.
• W2580758420 cites W2192080449 @default.
• W2580758420 cites W2223798378 @default.
• W2580758420 cites W2289724655 @default.
• W2580758420 cites W2299561689 @default.
• W2580758420 cites W30455059 @default.
• W2580758420 cites W4293247451 @default.
• W2580758420 doi "https://doi.org/10.1038/srep40851" @default.
• W2580758420 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5269589" @default.
• W2580758420 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28128232" @default.
• W2580758420 hasPublicationYear "2017" @default.
• W2580758420 type Work @default.
• W2580758420 sameAs 2580758420 @default.
• W2580758420 citedByCount "13" @default.
• W2580758420 countsByYear W25807584202017 @default.
• W2580758420 countsByYear W25807584202018 @default.
• W2580758420 countsByYear W25807584202020 @default.
• W2580758420 countsByYear W25807584202021 @default.
• W2580758420 countsByYear W25807584202022 @default.
• W2580758420 countsByYear W25807584202023 @default.
• W2580758420 crossrefType "journal-article" @default.
• W2580758420 hasAuthorship W2580758420A5019190057 @default.
• W2580758420 hasAuthorship W2580758420A5030202287 @default.
• W2580758420 hasAuthorship W2580758420A5038399698 @default.
• W2580758420 hasAuthorship W2580758420A5047721072 @default.
• W2580758420 hasAuthorship W2580758420A5072035000 @default.
• W2580758420 hasAuthorship W2580758420A5081788625 @default.
• W2580758420 hasAuthorship W2580758420A5082816482 @default.
• W2580758420 hasAuthorship W2580758420A5089822225 @default.
• W2580758420 hasAuthorship W2580758420A5090642719 @default.
• W2580758420 hasBestOaLocation W25807584201 @default.
• W2580758420 hasConcept C142724271 @default.
• W2580758420 hasConcept C181199279 @default.
• W2580758420 hasConcept C185592680 @default.
• W2580758420 hasConcept C190283241 @default.
• W2580758420 hasConcept C194671627 @default.
• W2580758420 hasConcept C204787440 @default.
• W2580758420 hasConcept C206103511 @default.
• W2580758420 hasConcept C2775933503 @default.
• W2580758420 hasConcept C2776211264 @default.
• W2580758420 hasConcept C2777228702 @default.
• W2580758420 hasConcept C2777278459 @default.
• W2580758420 hasConcept C2778905741 @default.
• W2580758420 hasConcept C2779196830 @default.
• W2580758420 hasConcept C2779222958 @default.
• W2580758420 hasConcept C2779729912 @default.
• W2580758420 hasConcept C2780695664 @default.
• W2580758420 hasConcept C2781079059 @default.
• W2580758420 hasConcept C2781164371 @default.
• W2580758420 hasConcept C553450214 @default.
• W2580758420 hasConcept C55493867 @default.
• W2580758420 hasConcept C59822182 @default.

• next