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• W2580877515 abstract "SensiTemp, a new IMV bull straw concept, presents the advantage of color changing while the straw is thawed. The color of frozen straws is blue and straws start to become white when the temperature reaches 33°C, with a complete change of color at 37°C. The objective of this study is to assess quality after thawing of semen frozen in SensiTemp, in vitro, using Computer Assisted Semen Analysis (CASA), Flow Cytometry (FC) and In Vitro Fertilization (IVF). The ejaculates of two bulls, selected during preliminary experiments on high in vitro fertility, were harvested at CIA L’Aigle, France and split ejaculates were frozen in experimental (SensiTemp) and conventional (Control) straws. In experiment 1 after thawing semen from the two type of straws (5 pooled straws each; 2 replicates), motility was assessed using the IVOS CASA system (Hamilton Thorne Inc., Beverly, MA, USA) and membrane integrity was evaluated through FC with Cytosoft software (Millipore-Guava Technologies Inc., Hayward, CA). In experiment 2, IVF was used to evaluate the non toxicity of SensiTemp and control straws. Cumulus-oocyte complexes (COC; n=1178; 4 replicates) collected from slaughterhouse ovaries were matured in IVM medium (TCM-199 with bicarbonate, Sigma-Aldrich, Saint Quentin Fallavier, France; 10µg/ml FSH-LH, Reprobiol, Liege, Belgium and 10% FCS, Thermo Fisher, Illkirch, France) for 22 h. After fertilization, presumptive zygotes of each group (SensiTemp and control for each bull) were cultured in synthetic oviduct fluid medium (SOF, Minitube, Tiefenbach, Germany) with 1% ECS and 0.6% BSA (Sigma-Aldrich, France) up to 8 days. All cultures were conducted at 38.5C in 5%CO2, 5%O2. The cleavage and blastocysts rates were evaluated on Day 3 and 7, respectively for each group. Embryo quality was recorded on day 7 according to the IETS evaluation. Data from each bull were analyzed separately using the Chi square test (P 0.05) in cleavage rate between SensiTemp and Control for the two bulls: 92.1 and 91.7% for bull 1 and 94.2 and 94.6% for bull 2 respectively. The blastocysts rate on day 7 did not differ (P>0.05) among groups (47.5, 47.1 and 51.3, 50.4% for SensiTemp and Control bull 1 and bull 2, respectively) nor the quality of embryos retrieved in the different groups: 25.4, 23.3 and 30.8, 29.6% in grade 1 embryo for SensiTemp and Control bull 1 and bull 2, respectively. Those results demonstrate, in vitro, that the new SensiTemp straws were non toxic and did not affect the semen quality after thawing nor did the SensiTemp straws affect the ability of sperm cells to fertilize oocytes and produce 8 days old embryos." @default.
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• W2580877515 date "2015-03-01" @default.
• W2580877515 modified "2023-09-23" @default.
• W2580877515 title "Assessment of bull semen quality loaded in new SensiTemp straws using semen and IVP technologies" @default.
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