FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2581106675> ?p ?o ?g. }

• W2581106675 endingPage "71" @default.
• W2581106675 startingPage "59" @default.
• W2581106675 abstract "MRP RNA is an abundant, essential noncoding RNA whose functions have been proposed in yeast but are incompletely understood in humans. Mutations in the genomic locus for MRP RNA cause pleiotropic human diseases, including cartilage hair hypoplasia (CHH). Here we applied CRISPR–Cas9 genome editing to disrupt the endogenous human MRP RNA locus, thereby attaining what has eluded RNAi and RNase H experiments: elimination of MRP RNA in the majority of cells. The resulting accumulation of ribosomal RNA (rRNA) precursor—analyzed by RNA fluorescent in situ hybridization (FISH), Northern blots, and RNA sequencing—implicates MRP RNA in pre-rRNA processing. Amelioration of pre-rRNA imbalance is achieved through rescue of MRP RNA levels by ectopic expression. Furthermore, affinity-purified MRP ribonucleoprotein (RNP) from HeLa cells cleaves the human pre-rRNA in vitro at at least one site used in cells, while RNP isolated from cells with CRISPR-edited MRP loci loses this activity, and ectopic MRP RNA expression restores cleavage activity. Thus, a role for RNase MRP in human pre-rRNA processing is established. As demonstrated here, targeted CRISPR disruption is a valuable tool for functional studies of essential noncoding RNAs that are resistant to RNAi and RNase H-based degradation." @default.
• W2581106675 created "2017-02-03" @default.
• W2581106675 creator A5017411235 @default.
• W2581106675 creator A5017573996 @default.
• W2581106675 date "2017-01-01" @default.
• W2581106675 modified "2023-10-17" @default.
• W2581106675 title "Targeted CRISPR disruption reveals a role for RNase MRP RNA in human preribosomal RNA processing" @default.
• W2581106675 cites W1557288216 @default.
• W2581106675 cites W1585970141 @default.
• W2581106675 cites W185028867 @default.
• W2581106675 cites W1977709885 @default.
• W2581106675 cites W1982000453 @default.
• W2581106675 cites W1990630809 @default.
• W2581106675 cites W1990728920 @default.
• W2581106675 cites W1996851109 @default.
• W2581106675 cites W2001377906 @default.
• W2581106675 cites W2021333801 @default.
• W2581106675 cites W2021666355 @default.
• W2581106675 cites W2022605680 @default.
• W2581106675 cites W2023037474 @default.
• W2581106675 cites W2041157094 @default.
• W2581106675 cites W2046559761 @default.
• W2581106675 cites W2046901323 @default.
• W2581106675 cites W2048160314 @default.
• W2581106675 cites W2080055531 @default.
• W2581106675 cites W2081155000 @default.
• W2581106675 cites W2082009457 @default.
• W2581106675 cites W2090955163 @default.
• W2581106675 cites W2093714761 @default.
• W2581106675 cites W2099814921 @default.
• W2581106675 cites W2105049718 @default.
• W2581106675 cites W2109406098 @default.
• W2581106675 cites W2118222551 @default.
• W2581106675 cites W2118609877 @default.
• W2581106675 cites W2128060252 @default.
• W2581106675 cites W2135532291 @default.
• W2581106675 cites W2141172277 @default.
• W2581106675 cites W2166784156 @default.
• W2581106675 cites W2169853432 @default.
• W2581106675 cites W2170280012 @default.
• W2581106675 cites W2170819005 @default.
• W2581106675 cites W2206191771 @default.
• W2581106675 cites W4241965353 @default.
• W2581106675 doi "https://doi.org/10.1101/gad.286963.116" @default.
• W2581106675 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5287113" @default.
• W2581106675 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28115465" @default.
• W2581106675 hasPublicationYear "2017" @default.
• W2581106675 type Work @default.
• W2581106675 sameAs 2581106675 @default.
• W2581106675 citedByCount "86" @default.
• W2581106675 countsByYear W25811066752017 @default.
• W2581106675 countsByYear W25811066752018 @default.
• W2581106675 countsByYear W25811066752019 @default.
• W2581106675 countsByYear W25811066752020 @default.
• W2581106675 countsByYear W25811066752021 @default.
• W2581106675 countsByYear W25811066752022 @default.
• W2581106675 countsByYear W25811066752023 @default.
• W2581106675 crossrefType "journal-article" @default.
• W2581106675 hasAuthorship W2581106675A5017411235 @default.
• W2581106675 hasAuthorship W2581106675A5017573996 @default.
• W2581106675 hasBestOaLocation W25811066751 @default.
• W2581106675 hasConcept C104317684 @default.
• W2581106675 hasConcept C10879258 @default.
• W2581106675 hasConcept C132455925 @default.
• W2581106675 hasConcept C153911025 @default.
• W2581106675 hasConcept C166703698 @default.
• W2581106675 hasConcept C172827444 @default.
• W2581106675 hasConcept C194993378 @default.
• W2581106675 hasConcept C195500695 @default.
• W2581106675 hasConcept C21786251 @default.
• W2581106675 hasConcept C2191507 @default.
• W2581106675 hasConcept C41258723 @default.
• W2581106675 hasConcept C54355233 @default.
• W2581106675 hasConcept C67705224 @default.
• W2581106675 hasConcept C67905577 @default.
• W2581106675 hasConcept C86803240 @default.
• W2581106675 hasConcept C95444343 @default.
• W2581106675 hasConcept C97702854 @default.
• W2581106675 hasConcept C98108389 @default.
• W2581106675 hasConceptScore W2581106675C104317684 @default.
• W2581106675 hasConceptScore W2581106675C10879258 @default.
• W2581106675 hasConceptScore W2581106675C132455925 @default.
• W2581106675 hasConceptScore W2581106675C153911025 @default.
• W2581106675 hasConceptScore W2581106675C166703698 @default.
• W2581106675 hasConceptScore W2581106675C172827444 @default.
• W2581106675 hasConceptScore W2581106675C194993378 @default.
• W2581106675 hasConceptScore W2581106675C195500695 @default.
• W2581106675 hasConceptScore W2581106675C21786251 @default.
• W2581106675 hasConceptScore W2581106675C2191507 @default.
• W2581106675 hasConceptScore W2581106675C41258723 @default.
• W2581106675 hasConceptScore W2581106675C54355233 @default.
• W2581106675 hasConceptScore W2581106675C67705224 @default.
• W2581106675 hasConceptScore W2581106675C67905577 @default.
• W2581106675 hasConceptScore W2581106675C86803240 @default.
• W2581106675 hasConceptScore W2581106675C95444343 @default.
• W2581106675 hasConceptScore W2581106675C97702854 @default.
• W2581106675 hasConceptScore W2581106675C98108389 @default.
• W2581106675 hasFunder F4320332161 @default.

• next