FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2581588529> ?p ?o ?g. }

• W2581588529 abstract "The CRISPR/Cas9 system enables precision editing of the genome of the model plant Arabidopsis thaliana (Arabidopsis) and likely of any other organism. Tools and methods for further developing and optimizing this widespread and versatile system in Arabidopsis would hence be welcomed. Here we designed a generic vector system that can be used to clone any sgRNA sequence in a plant T-DNA vector containing an ubiquitously expressed Cas9 gene. With this vector, we explored two alternative marker systems for tracking Cas9-mediated gene-editing in vivo: BIALAPHOS RESISTANCE (BAR) and GLABROUS1 (GL1). BAR confers resistance to glufosinate and is widely used as a positive selection marker; GL1 is required for the formation of trichomes. Reversion of a frameshift null BAR allele to a functional one by Cas9-mediated gene editing yielded a higher than expected number of plants that are resistant to glufosinate. Surprisingly, many of those plants did not display reversion of the BAR gene through the germline. We hypothesize that few BAR revertant cells in a highly chimeric plant likely provide system wide resistance to glufosinate and thus we suggest that BAR is not suitable as marker for tracking Cas9-mediated gene-editing. Targeting the GL1 gene for disruption with Cas9 provided clearly visible phenotypes of partially and completely glabrous plants. 50% of the analyzed T1 plants produced descendants with a chimeric phenotype and we could recover fully homozygous plants in the T3 generation with high efficiency. We propose that targeting of GL1 is suitable for assessing and optimizing Cas9-mediated gene-editing in Arabidopsis." @default.
• W2581588529 created "2017-02-03" @default.
• W2581588529 creator A5032787107 @default.
• W2581588529 creator A5039062641 @default.
• W2581588529 creator A5063176250 @default.
• W2581588529 creator A5063978500 @default.
• W2581588529 creator A5068924966 @default.
• W2581588529 creator A5079672202 @default.
• W2581588529 date "2017-01-24" @default.
• W2581588529 modified "2023-09-25" @default.
• W2581588529 title "An Efficient Visual Screen for CRISPR/Cas9 Activity in Arabidopsis thaliana" @default.
• W2581588529 cites W1787401975 @default.
• W2581588529 cites W1859210412 @default.
• W2581588529 cites W1966115988 @default.
• W2581588529 cites W1977709885 @default.
• W2581588529 cites W1980007971 @default.
• W2581588529 cites W1980201684 @default.
• W2581588529 cites W1981705025 @default.
• W2581588529 cites W1996341895 @default.
• W2581588529 cites W1996390333 @default.
• W2581588529 cites W2003231662 @default.
• W2581588529 cites W2007614581 @default.
• W2581588529 cites W2017746652 @default.
• W2581588529 cites W2024199022 @default.
• W2581588529 cites W2032764699 @default.
• W2581588529 cites W2035927503 @default.
• W2581588529 cites W2037043005 @default.
• W2581588529 cites W2045435533 @default.
• W2581588529 cites W2050480410 @default.
• W2581588529 cites W2052192736 @default.
• W2581588529 cites W2068502244 @default.
• W2581588529 cites W2077659966 @default.
• W2581588529 cites W2081261052 @default.
• W2581588529 cites W2087918598 @default.
• W2581588529 cites W2090993318 @default.
• W2581588529 cites W2094600083 @default.
• W2581588529 cites W2105464583 @default.
• W2581588529 cites W2109560939 @default.
• W2581588529 cites W2113224678 @default.
• W2581588529 cites W2115603949 @default.
• W2581588529 cites W2116122104 @default.
• W2581588529 cites W2128836133 @default.
• W2581588529 cites W2132188193 @default.
• W2581588529 cites W2141233420 @default.
• W2581588529 cites W2143142471 @default.
• W2581588529 cites W2143664231 @default.
• W2581588529 cites W2144503155 @default.
• W2581588529 cites W2150273306 @default.
• W2581588529 cites W2150633041 @default.
• W2581588529 cites W2154778106 @default.
• W2581588529 cites W2157488912 @default.
• W2581588529 cites W2158215557 @default.
• W2581588529 cites W2159048608 @default.
• W2581588529 cites W2160714550 @default.
• W2581588529 cites W2163820831 @default.
• W2581588529 cites W2166396989 @default.
• W2581588529 cites W2167290867 @default.
• W2581588529 cites W2175442085 @default.
• W2581588529 cites W2229991088 @default.
• W2581588529 cites W2287494390 @default.
• W2581588529 cites W2313484582 @default.
• W2581588529 cites W2479896437 @default.
• W2581588529 cites W2507138770 @default.
• W2581588529 cites W4244430438 @default.
• W2581588529 cites W90532991 @default.
• W2581588529 doi "https://doi.org/10.3389/fpls.2017.00039" @default.
• W2581588529 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5258748" @default.
• W2581588529 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28174584" @default.
• W2581588529 hasPublicationYear "2017" @default.
• W2581588529 type Work @default.
• W2581588529 sameAs 2581588529 @default.
• W2581588529 citedByCount "35" @default.
• W2581588529 countsByYear W25815885292017 @default.
• W2581588529 countsByYear W25815885292018 @default.
• W2581588529 countsByYear W25815885292019 @default.
• W2581588529 countsByYear W25815885292020 @default.
• W2581588529 countsByYear W25815885292021 @default.
• W2581588529 countsByYear W25815885292022 @default.
• W2581588529 crossrefType "journal-article" @default.
• W2581588529 hasAuthorship W2581588529A5032787107 @default.
• W2581588529 hasAuthorship W2581588529A5039062641 @default.
• W2581588529 hasAuthorship W2581588529A5063176250 @default.
• W2581588529 hasAuthorship W2581588529A5063978500 @default.
• W2581588529 hasAuthorship W2581588529A5068924966 @default.
• W2581588529 hasAuthorship W2581588529A5079672202 @default.
• W2581588529 hasBestOaLocation W25815885291 @default.
• W2581588529 hasConcept C104317684 @default.
• W2581588529 hasConcept C132455925 @default.
• W2581588529 hasConcept C143065580 @default.
• W2581588529 hasConcept C144501496 @default.
• W2581588529 hasConcept C22744801 @default.
• W2581588529 hasConcept C2775988993 @default.
• W2581588529 hasConcept C2779491563 @default.
• W2581588529 hasConcept C2908719506 @default.
• W2581588529 hasConcept C42411736 @default.
• W2581588529 hasConcept C54355233 @default.
• W2581588529 hasConcept C553450214 @default.
• W2581588529 hasConcept C70721500 @default.
• W2581588529 hasConcept C86803240 @default.
• W2581588529 hasConcept C98108389 @default.

• next