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• W2582804558 abstract "Single domain antibodies are recombinantly expressed variable domains derived from camelid heavy chain antibodies. Natural single domain antibodies can have noncanonical disulfide bonds between their complementarity-determining regions that help position the binding site. In addition, engineering a second disulfide bond serves to tie together β-sheets thereby inhibiting unfolding. Unfortunately, the additional disulfide bond often significantly decreases yield, presumably due to formation of incorrect disulfide bonds during the folding process. Here, we demonstrate that inclusion of the helper plasmid pTUM4, which results in the expression of four chaperones, DsbA, DsbC, FkpA, and SurA, increased yield on average 3.5-fold for the nine multi-disulfide bond single domain antibodies evaluated. No increase in production was observed for single domain antibodies containing only the canonical disulfide bond." @default.
• W2582804558 created "2017-02-03" @default.
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• W2582804558 date "2017-04-01" @default.
• W2582804558 modified "2023-09-23" @default.
• W2582804558 title "Improved production of single domain antibodies with two disulfide bonds by co-expression of chaperone proteins in the Escherichia coli periplasm" @default.
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• W2582804558 doi "https://doi.org/10.1016/j.jim.2017.01.007" @default.
• W2582804558 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28131818" @default.
• W2582804558 hasPublicationYear "2017" @default.
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