Matches in SemOpenAlex for { <https://semopenalex.org/work/W2582832066> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W2582832066 abstract "The present study aimed to diagnose Babesia bigemina in naturally infected crossbred cows and to determine its effect on haemato-biochemical profile of host animals. Blood samples from lactating crossbred cows (n=30) between 3-6 years of age and showing clinical signs of babesiosis were collected, with or without anticoagulant, and analyzed for the protozoa by direct smear, direct blood PCR detection of the apical membrane antigen 1 (AMA-1) gene specific amplicon of B. bigemina and estimation of haematological and biochemical parameters. Healthy crossbred cows (n=10), examined free from haemoprotozoan infections were included as control. Blood Direct PCR revealed a 448-bp amplified fragment. Out of 150 random blood samples screened, (27/150) 18% were positive under light microscope, whereas direct blood PCR revealed (39/150) 26% samples positive for B. bigemina. The result shows higher specificity and sensitivity of PCR test over blood smear examination. The infected group showed significantly (p less than 0.001) decreased levels of TEC (3.04±0.19), Hb (4.78±0.27) and PCV (14.53 ±0.87) than healthy control animals. However, differences in the red blood cell indices (MCV, MCH and MCHC) were non-significant (p>0.05) between the groups indicating normocytic hypochromic anaemia in affected crossbred cattle. Serum samples of infected cows showed significantly (p less than 0.01) higher values of ALT (78.83±8.95), AST (146.13±7.62), BUN (27.09±1.02), creatinine (1.93±0.1) and TBIL (1.42±0.06) than that of healthy control. A significant decrease (p less than 0.01) of TSP (6.12±0.13) and albumin (2.39±0.09) was also recorded in the infected cows compare to healthy control. The standardized blood direct PCR method of the present investigation may be useful for rapid and reliable diagnosis of B. bigemina in conjunction with microscopic examination. Moreover, marked changes in haematological and serum biochemical profile observed in B. bigemina infected crossbred cows may be useful in understanding disease pathogenesis and undertaking necessary corrective measures.." @default.
- W2582832066 created "2017-02-03" @default.
- W2582832066 creator A5007231660 @default.
- W2582832066 creator A5018851738 @default.
- W2582832066 creator A5019277045 @default.
- W2582832066 creator A5025235909 @default.
- W2582832066 creator A5074135861 @default.
- W2582832066 creator A5076891294 @default.
- W2582832066 date "2017-01-12" @default.
- W2582832066 modified "2023-10-03" @default.
- W2582832066 title "Direct blood PCR detection of Babesia bigemina and its effect on haematological and biochemical profile in crossbred cattle of eastern Haryana" @default.
- W2582832066 doi "https://doi.org/10.18805/ijar.v0iof.7007" @default.
- W2582832066 hasPublicationYear "2017" @default.
- W2582832066 type Work @default.
- W2582832066 sameAs 2582832066 @default.
- W2582832066 citedByCount "7" @default.
- W2582832066 countsByYear W25828320662018 @default.
- W2582832066 countsByYear W25828320662019 @default.
- W2582832066 countsByYear W25828320662020 @default.
- W2582832066 countsByYear W25828320662023 @default.
- W2582832066 crossrefType "journal-article" @default.
- W2582832066 hasAuthorship W2582832066A5007231660 @default.
- W2582832066 hasAuthorship W2582832066A5018851738 @default.
- W2582832066 hasAuthorship W2582832066A5019277045 @default.
- W2582832066 hasAuthorship W2582832066A5025235909 @default.
- W2582832066 hasAuthorship W2582832066A5074135861 @default.
- W2582832066 hasAuthorship W2582832066A5076891294 @default.
- W2582832066 hasBestOaLocation W25828320661 @default.
- W2582832066 hasConcept C126322002 @default.
- W2582832066 hasConcept C140793950 @default.
- W2582832066 hasConcept C173419221 @default.
- W2582832066 hasConcept C203014093 @default.
- W2582832066 hasConcept C2778048844 @default.
- W2582832066 hasConcept C2778145968 @default.
- W2582832066 hasConcept C2780306776 @default.
- W2582832066 hasConcept C2780581805 @default.
- W2582832066 hasConcept C2910449918 @default.
- W2582832066 hasConcept C3017819844 @default.
- W2582832066 hasConcept C42972112 @default.
- W2582832066 hasConcept C71924100 @default.
- W2582832066 hasConcept C86803240 @default.
- W2582832066 hasConceptScore W2582832066C126322002 @default.
- W2582832066 hasConceptScore W2582832066C140793950 @default.
- W2582832066 hasConceptScore W2582832066C173419221 @default.
- W2582832066 hasConceptScore W2582832066C203014093 @default.
- W2582832066 hasConceptScore W2582832066C2778048844 @default.
- W2582832066 hasConceptScore W2582832066C2778145968 @default.
- W2582832066 hasConceptScore W2582832066C2780306776 @default.
- W2582832066 hasConceptScore W2582832066C2780581805 @default.
- W2582832066 hasConceptScore W2582832066C2910449918 @default.
- W2582832066 hasConceptScore W2582832066C3017819844 @default.
- W2582832066 hasConceptScore W2582832066C42972112 @default.
- W2582832066 hasConceptScore W2582832066C71924100 @default.
- W2582832066 hasConceptScore W2582832066C86803240 @default.
- W2582832066 hasIssue "OF" @default.
- W2582832066 hasLocation W25828320661 @default.
- W2582832066 hasOpenAccess W2582832066 @default.
- W2582832066 hasPrimaryLocation W25828320661 @default.
- W2582832066 hasRelatedWork W1487084772 @default.
- W2582832066 hasRelatedWork W1589454801 @default.
- W2582832066 hasRelatedWork W2041495734 @default.
- W2582832066 hasRelatedWork W2209161297 @default.
- W2582832066 hasRelatedWork W2237333613 @default.
- W2582832066 hasRelatedWork W2337104398 @default.
- W2582832066 hasRelatedWork W2403972088 @default.
- W2582832066 hasRelatedWork W3093709441 @default.
- W2582832066 hasRelatedWork W4384785832 @default.
- W2582832066 hasRelatedWork W2945218089 @default.
- W2582832066 isParatext "false" @default.
- W2582832066 isRetracted "false" @default.
- W2582832066 magId "2582832066" @default.
- W2582832066 workType "article" @default.