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- W2582873639 abstract "We show that the three conformational states of integrin α5β1 have discrete free energies and define activation by measuring intrinsic affinities for ligand of each state and the equilibria linking them. The 5,000‐fold higher affinity of the extended‐open state than the bent‐closed and extended‐closed states demonstrates profound regulation of affinity. Free energy requirements for activation are defined with protein fragments and intact α5β1. On the surface of K562 cells, α5β1 is 99.8% bent‐closed. Stabilization of the bent conformation by integrin transmembrane and cytoplasmic domains must be overcome by cellular energy input to stabilize extension. Following extension, headpiece opening is energetically favored. N‐glycans and leg domains in each subunit that connect the ligand‐binding head to the membrane repel or crowd one another and regulate conformational equilibria in favor of headpiece opening. The results suggest new principles for regulating signaling in the large class of receptors built from extracellular domains in tandem with single‐span transmembrane domains.![][1] Thermodynamic analysis of the three integrin α5β1 conformation states offers a model for signaling regulation via an interplay between extracellular and transmembrane/intracellular domains in a large class of receptors built from multiple domains. [1]: /embed/graphic-1.gif" @default.
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- W2582873639 date "2017-01-25" @default.
- W2582873639 modified "2023-10-05" @default.
- W2582873639 title "Conformational equilibria and intrinsic affinities define integrin activation" @default.
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- W2582873639 doi "https://doi.org/10.15252/embj.201695803" @default.
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!["We show that the three conformational states of integrin α5β1 have discrete free energies and define activation by measuring intrinsic affinities for ligand of each state and the equilibria linking them. The 5,000‐fold higher affinity of the extended‐open state than the bent‐closed and extended‐closed states demonstrates profound regulation of affinity. Free energy requirements for activation are defined with protein fragments and intact α5β1. On the surface of K562 cells, α5β1 is 99.8% bent‐closed. Stabilization of the bent conformation by integrin transmembrane and cytoplasmic domains must be overcome by cellular energy input to stabilize extension. Following extension, headpiece opening is energetically favored. N‐glycans and leg domains in each subunit that connect the ligand‐binding head to the membrane repel or crowd one another and regulate conformational equilibria in favor of headpiece opening. The results suggest new principles for regulating signaling in the large class of receptors built from extracellular domains in tandem with single‐span transmembrane domains.![][1] Thermodynamic analysis of the three integrin α5β1 conformation states offers a model for signaling regulation via an interplay between extracellular and transmembrane/intracellular domains in a large class of receptors built from multiple domains. [1]: /embed/graphic-1.gif"](?object=%22We%20show%20that%20the%20three%20conformational%20states%20of%20integrin%20%CE%B15%CE%B21%20have%20discrete%20free%20energies%20and%20define%20activation%20by%20measuring%20intrinsic%20affinities%20for%20ligand%20of%20each%20state%20and%20the%20equilibria%20linking%20them.%20The%205%2C000%E2%80%90fold%20higher%20affinity%20of%20the%20extended%E2%80%90open%20state%20than%20the%20bent%E2%80%90closed%20and%20extended%E2%80%90closed%20states%20demonstrates%20profound%20regulation%20of%20affinity.%20Free%20energy%20requirements%20for%20activation%20are%20defined%20with%20protein%20fragments%20and%20intact%20%CE%B15%CE%B21.%20On%20the%20surface%20of%20K562%20cells%2C%20%CE%B15%CE%B21%20is%2099.8%25%20bent%E2%80%90closed.%20Stabilization%20of%20the%20bent%20conformation%20by%20integrin%20transmembrane%20and%20cytoplasmic%20domains%20must%20be%20overcome%20by%20cellular%20energy%20input%20to%20stabilize%20extension.%20Following%20extension%2C%20headpiece%20opening%20is%20energetically%20favored.%20N%E2%80%90glycans%20and%20leg%20domains%20in%20each%20subunit%20that%20connect%20the%20ligand%E2%80%90binding%20head%20to%20the%20membrane%20repel%20or%20crowd%20one%20another%20and%20regulate%20conformational%20equilibria%20in%20favor%20of%20headpiece%20opening.%20The%20results%20suggest%20new%20principles%20for%20regulating%20signaling%20in%20the%20large%20class%20of%20receptors%20built%20from%20extracellular%20domains%20in%20tandem%20with%20single%E2%80%90span%20transmembrane%20domains.!%5B%5D%5B1%5D%20Thermodynamic%20analysis%20of%20the%20three%20integrin%20%CE%B15%CE%B21%20conformation%20states%20offers%20a%20model%20for%20signaling%20regulation%20via%20an%20interplay%20between%20extracellular%20and%20transmembrane%2Fintracellular%20domains%20in%20a%20large%20class%20of%20receptors%20built%20from%20multiple%20domains.%20%5B1%5D%3A%20%2Fembed%2Fgraphic-1.gif%22){kind=link}