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• W2582902105 abstract "ObjectiveThe protocol of interphase-nuclear-conversion induced by fusion of a blastomere with a mouse zygote has been set up as a method of preimplantation genetic diagnosis(PGD) to detect the chromosomal imbalance in the embryos of translocation carriers. But it was found that metaphase chromosome could not be obtained in some cells. The reasons maybe the fertilization of a mouse oocyte with an abnormal sperm, or self activation occuring in aged oocytes, all of which may compromise the cleavage potential of the heterokaryons and then lead to the failure of the conversion. In this study, we used fresh mouse oocytes activated by SrCl2 to induce the nuclear conversion, and compared the efficacy with that of mouse zygotes used.DesignA randomized, prospective analysis.Materials and methods227 blastomeres from 61 untransfered human embryos in our reproductive center were used in the study and divided into two groups randomly, one was fused with mouse zygotes(103 blastomeres, Z-group) and the other with fresh mouse oocytes parthenogenetic activated by SrCl2(124 blastomeres, P-group). When the nuclear envelop of the heterokaryon disappeared, the cell was fixed and observed under the microscope for chromosome. In the end, the fusion rate, chromosome conversion rate and time required for conversion were compared between these two groups.The use of the untransfered embryos has got consent from every patient, and the study has been approved by the Ethical Review Board of our hospital.ResultsTabled 1Tabled 1ConclusionThere is no significant difference in fusion rate and time required for chromosome conversion between the parthenogenetic activation group and the zygote group, while the chromosome conversion rate is higher in the former group and the range of mean time required is broader in the latter, which implies that parthenogenetic activated fresh mouse oocytes could be a better alternative for induction of premature condensed chromosome, especially for PGD. ObjectiveThe protocol of interphase-nuclear-conversion induced by fusion of a blastomere with a mouse zygote has been set up as a method of preimplantation genetic diagnosis(PGD) to detect the chromosomal imbalance in the embryos of translocation carriers. But it was found that metaphase chromosome could not be obtained in some cells. The reasons maybe the fertilization of a mouse oocyte with an abnormal sperm, or self activation occuring in aged oocytes, all of which may compromise the cleavage potential of the heterokaryons and then lead to the failure of the conversion. In this study, we used fresh mouse oocytes activated by SrCl2 to induce the nuclear conversion, and compared the efficacy with that of mouse zygotes used. The protocol of interphase-nuclear-conversion induced by fusion of a blastomere with a mouse zygote has been set up as a method of preimplantation genetic diagnosis(PGD) to detect the chromosomal imbalance in the embryos of translocation carriers. But it was found that metaphase chromosome could not be obtained in some cells. The reasons maybe the fertilization of a mouse oocyte with an abnormal sperm, or self activation occuring in aged oocytes, all of which may compromise the cleavage potential of the heterokaryons and then lead to the failure of the conversion. In this study, we used fresh mouse oocytes activated by SrCl2 to induce the nuclear conversion, and compared the efficacy with that of mouse zygotes used. DesignA randomized, prospective analysis. A randomized, prospective analysis. Materials and methods227 blastomeres from 61 untransfered human embryos in our reproductive center were used in the study and divided into two groups randomly, one was fused with mouse zygotes(103 blastomeres, Z-group) and the other with fresh mouse oocytes parthenogenetic activated by SrCl2(124 blastomeres, P-group). When the nuclear envelop of the heterokaryon disappeared, the cell was fixed and observed under the microscope for chromosome. In the end, the fusion rate, chromosome conversion rate and time required for conversion were compared between these two groups.The use of the untransfered embryos has got consent from every patient, and the study has been approved by the Ethical Review Board of our hospital. 227 blastomeres from 61 untransfered human embryos in our reproductive center were used in the study and divided into two groups randomly, one was fused with mouse zygotes(103 blastomeres, Z-group) and the other with fresh mouse oocytes parthenogenetic activated by SrCl2(124 blastomeres, P-group). When the nuclear envelop of the heterokaryon disappeared, the cell was fixed and observed under the microscope for chromosome. In the end, the fusion rate, chromosome conversion rate and time required for conversion were compared between these two groups.The use of the untransfered embryos has got consent from every patient, and the study has been approved by the Ethical Review Board of our hospital. ResultsTabled 1Tabled 1 ConclusionThere is no significant difference in fusion rate and time required for chromosome conversion between the parthenogenetic activation group and the zygote group, while the chromosome conversion rate is higher in the former group and the range of mean time required is broader in the latter, which implies that parthenogenetic activated fresh mouse oocytes could be a better alternative for induction of premature condensed chromosome, especially for PGD. There is no significant difference in fusion rate and time required for chromosome conversion between the parthenogenetic activation group and the zygote group, while the chromosome conversion rate is higher in the former group and the range of mean time required is broader in the latter, which implies that parthenogenetic activated fresh mouse oocytes could be a better alternative for induction of premature condensed chromosome, especially for PGD." @default.
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• W2582902105 date "2006-09-01" @default.
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• W2582902105 doi "https://doi.org/10.1016/j.fertnstert.2006.07.157" @default.
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