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- W2583006977 abstract "Objective: To establish a laryngeal squamous cell carcinoma (LSCC) cell line through primary cell culture and observe its biological characteristics. Methods: Tissue block culture method was used for primary cell culture. After LSCC cells passed 25 times in vitro, the morphology of cells was observed, keratin was stained histochemically, cell cycle was tested by PI-FACS, and the specie of cells was detected by PCR and short tandem repeat(STR) typing. Results: This newly established LSCC cell line was named as TR-LCC-1, most of the cancer cells were polygonal shape, like the cobblestone, loss of contact inhibition and with overlapping growth. Cell size was large and cell pleomorphism was very obvious. Cytokeratin staining was positive. After 6 months of continuous culture in vitro, the TR-LCC-1 cells passed more than 30 times, and cell doubling time was 201.2h. Cell cycle assay indicated that G1 phase accounted for 51.71%, S phase was 44.56%, and G2 phase was 2.28%. Mycoplasma test showed no mycoplasma contamination. Cell species identification identified TR-LCC-1 was human-derived cells. STR detection showed P26 and P6 were same, and they were different from the STR typing of disclosed cells. Conclusion: The establish ment of the new laryngeal squamous carcinoma cell line TR-LCC-1 can be helpful to the research for laryngeal squamous cell cancer.目的： 原代培养喉鳞状细胞癌组织细胞，观察其生物学特性。 方法： 采用组织块法对喉鳞状细胞癌组织细胞进行原代培养，体外传代观察，传代25次以上对培养细胞进行形态学观察、角蛋白组织化学染色、细胞周期检测、培养法进行支原体检测、PCR法细胞种属鉴定、短串联重复序列(short tandem repeat, STR)分型。 结果： 新建立的喉鳞状细胞癌细胞系命名为TR-LCC-1，肿瘤细胞形态大部分呈多边形，似铺路石样排列，失去接触抑制而重叠生长，细胞体积大，异形性明显。细胞角蛋白染色阳性。体外连续培养6个月，传代30次以上，细胞倍增时间为201.2 h。细胞周期测定G1期为51.71%，S期为44.56%，G2期为2.28%。支原体检测无支原体污染。细胞种属鉴定TR-LCC-1为人来源细胞。STR检测分析P26与P6相同，且与已公开细胞的STR不同。 结论： 建立了来源于喉鳞状细胞癌的细胞系TR-LCC-1，可用于喉鳞状细胞癌的肿瘤实验研究。." @default.
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- W2583006977 date "2017-01-07" @default.
- W2583006977 modified "2023-10-18" @default.
- W2583006977 title "[Establishment and characterization of a laryngeal squamous cell carcinoma cell line]." @default.
- W2583006977 doi "https://doi.org/10.3760/cma.j.issn.1673-0860.2017.01.008" @default.
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