FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2583012913> ?p ?o ?g. }

• W2583012913 endingPage "1070" @default.
• W2583012913 startingPage "1061" @default.
• W2583012913 abstract "Aims To understand better the control of insulin secretion by human β cells and to identify similarities to and differences from rodent models. Methods Dynamic insulin secretion was measured in perifused human islets treated with pharmacological agents of known modes of action. Results Glucokinase activation ( R o28‐1675) lowered the glucose threshold for stimulation of insulin secretion to 1 mmol/ L ( G1 ), augmented the response to G3‐G5 but not to G8‐G15 , whereas tolbutamide remained active in G20 , which indicates that not all K ATP channels were closed by high glucose concentrations. An almost 2‐fold greater response to G15 than to supramaximal tolbutamide in G3 or to KCl +diazoxide in G15 vs G3 quantified the contribution of metabolic amplification to insulin secretion. Both disruption (latrunculin‐ B ) and stabilization (jasplakinolide) of microfilaments augmented insulin secretion without affecting metabolic amplification. Tolbutamide‐induced insulin secretion was consistently greater in G10 than G3 , with a threshold at 1 and maximum at 10 µmol/ L tolbutamide in G10 , vs 10 and 25 µmol/ L in G3 . Sulphonylurea effects were thus clearly glucose‐dependent. Insulin secretion was also increased by inhibiting K channels other than K ATP channels: K v or BK channels (tetraethylammonium), TASK ‐1 channels ( ML ‐365) and SK4 channels ( TRAM ‐34). Opening K ATP channels with diazoxide inhibited glucose‐induced insulin secretion with half maximum inhibitory concentrations of 9.6 and 24 µmol/ L at G7 and G15 . Blockade of L ‐type Ca channels (nimodipine) abolished insulin secretion, whereas a blocker of T ‐type C a channels ( NNC ‐55‐0396) was ineffective at specific concentrations. Blockade of N a channels (tetrodotoxin) did not affect glucose‐induced insulin secretion. Conclusions In addition to sharing a K ATP channel‐dependent triggering pathway and a metabolic amplifying pathway, human and rodent β cells were found to display more similarities than differences in the control of insulin secretion." @default.
• W2583012913 created "2017-02-03" @default.
• W2583012913 creator A5002152162 @default.
• W2583012913 creator A5040582776 @default.
• W2583012913 creator A5050215643 @default.
• W2583012913 creator A5076977607 @default.
• W2583012913 creator A5084330205 @default.
• W2583012913 date "2017-03-31" @default.
• W2583012913 modified "2023-10-18" @default.
• W2583012913 title "Pharmacological approach to understanding the control of insulin secretion in human islets" @default.
• W2583012913 cites W1746749941 @default.
• W2583012913 cites W1969165259 @default.
• W2583012913 cites W1969252369 @default.
• W2583012913 cites W1984755183 @default.
• W2583012913 cites W1985117435 @default.
• W2583012913 cites W1985592505 @default.
• W2583012913 cites W1988246532 @default.
• W2583012913 cites W1991003148 @default.
• W2583012913 cites W1991626544 @default.
• W2583012913 cites W1996701347 @default.
• W2583012913 cites W2013658326 @default.
• W2583012913 cites W2014744835 @default.
• W2583012913 cites W2015001567 @default.
• W2583012913 cites W2019993996 @default.
• W2583012913 cites W2032823928 @default.
• W2583012913 cites W2044911054 @default.
• W2583012913 cites W2045594545 @default.
• W2583012913 cites W2046025111 @default.
• W2583012913 cites W2061009859 @default.
• W2583012913 cites W2072984035 @default.
• W2583012913 cites W2075895806 @default.
• W2583012913 cites W2083829302 @default.
• W2583012913 cites W2101814428 @default.
• W2583012913 cites W2108585459 @default.
• W2583012913 cites W2110906674 @default.
• W2583012913 cites W2112662561 @default.
• W2583012913 cites W2113274746 @default.
• W2583012913 cites W2116424238 @default.
• W2583012913 cites W2125713853 @default.
• W2583012913 cites W2129483129 @default.
• W2583012913 cites W2132183811 @default.
• W2583012913 cites W2138078259 @default.
• W2583012913 cites W2140248834 @default.
• W2583012913 cites W2140402299 @default.
• W2583012913 cites W2140513053 @default.
• W2583012913 cites W2147284000 @default.
• W2583012913 cites W2154712565 @default.
• W2583012913 cites W2159222406 @default.
• W2583012913 cites W2163960099 @default.
• W2583012913 cites W2164306929 @default.
• W2583012913 cites W2186359402 @default.
• W2583012913 cites W2225917948 @default.
• W2583012913 cites W2463572644 @default.
• W2583012913 doi "https://doi.org/10.1111/dom.12887" @default.
• W2583012913 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28116849" @default.
• W2583012913 hasPublicationYear "2017" @default.
• W2583012913 type Work @default.
• W2583012913 sameAs 2583012913 @default.
• W2583012913 citedByCount "27" @default.
• W2583012913 countsByYear W25830129132017 @default.
• W2583012913 countsByYear W25830129132018 @default.
• W2583012913 countsByYear W25830129132019 @default.
• W2583012913 countsByYear W25830129132020 @default.
• W2583012913 countsByYear W25830129132021 @default.
• W2583012913 countsByYear W25830129132022 @default.
• W2583012913 crossrefType "journal-article" @default.
• W2583012913 hasAuthorship W2583012913A5002152162 @default.
• W2583012913 hasAuthorship W2583012913A5040582776 @default.
• W2583012913 hasAuthorship W2583012913A5050215643 @default.
• W2583012913 hasAuthorship W2583012913A5076977607 @default.
• W2583012913 hasAuthorship W2583012913A5084330205 @default.
• W2583012913 hasConcept C126322002 @default.
• W2583012913 hasConcept C134018914 @default.
• W2583012913 hasConcept C153461711 @default.
• W2583012913 hasConcept C165220095 @default.
• W2583012913 hasConcept C185592680 @default.
• W2583012913 hasConcept C2776054734 @default.
• W2583012913 hasConcept C2779306644 @default.
• W2583012913 hasConcept C2779872217 @default.
• W2583012913 hasConcept C49039625 @default.
• W2583012913 hasConcept C519063684 @default.
• W2583012913 hasConcept C71924100 @default.
• W2583012913 hasConcept C86803240 @default.
• W2583012913 hasConcept C98335778 @default.
• W2583012913 hasConceptScore W2583012913C126322002 @default.
• W2583012913 hasConceptScore W2583012913C134018914 @default.
• W2583012913 hasConceptScore W2583012913C153461711 @default.
• W2583012913 hasConceptScore W2583012913C165220095 @default.
• W2583012913 hasConceptScore W2583012913C185592680 @default.
• W2583012913 hasConceptScore W2583012913C2776054734 @default.
• W2583012913 hasConceptScore W2583012913C2779306644 @default.
• W2583012913 hasConceptScore W2583012913C2779872217 @default.
• W2583012913 hasConceptScore W2583012913C49039625 @default.
• W2583012913 hasConceptScore W2583012913C519063684 @default.
• W2583012913 hasConceptScore W2583012913C71924100 @default.
• W2583012913 hasConceptScore W2583012913C86803240 @default.
• W2583012913 hasConceptScore W2583012913C98335778 @default.
• W2583012913 hasFunder F4320306172 @default.

• next