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- W2583804501 abstract "Publisher Summary Cell-free transcription and translation is frequently used to gain information about the structure and activity of proteins encoded by cDNA clones. Hitherto, it has generally been applied to specific, individual clones, identified and isolated by some other means. The analogous procedure of microinjection of translationally competent RNA transcribed from collections of recombinants has, however, been used successfully in isolating clones encoding proteins with known biological activities. A general problem in cDNA cloning is the dilution of true recombinants in libraries by nonrecombinant genome types, lacking cDNA sequences. At present, most libraries are made with a view to recovering one or a few particular clones for which specific probes—nucleic acid or antibody—are available. The chapter describes vectors and technology appropriate to the analysis of large groups of clones along with the individual clones, in terms of their protein-coding capacity. This methodology can be used not only in searches for clones encoding specific proteins, but also in the analysis of complex mRNA populations. The chapter is concerned chiefly with technical details, but some applications of the methodology are also discussed." @default.
- W2583804501 created "2017-02-10" @default.
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- W2583804501 date "1993-01-01" @default.
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- W2583804501 title "[11] Cell-free expression of large collections of cDNA clones using positive-selection λ phage vectors" @default.
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- W2583804501 doi "https://doi.org/10.1016/0076-6879(93)17061-9" @default.
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