FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2584164846> ?p ?o ?g. }

• W2584164846 abstract "Proteogenomics is an emerging field at the intersection of genomics and proteomics. Many variant peptides corresponding to single nucleotide variations (SNVs) are associated with specific diseases. The aim of this study was to demonstrate the feasibility of proteogenomic-based variant peptide detection in disease models and clinical specimens.We sought to detect p53 single amino acid variant (SAAV) peptides in breast cancer tumor samples that have been previously subjected to sequencing analysis. Initially, two cancer cell lines having a cellular tumor antigen p53 (TP53) mutation and one wild type for TP53 were analyzed by selected reaction monitoring (SRM) assays as controls. One pool of wild type and one pool of mutated for TP53 cytosolic extracts were assayed with a shotgun proteogenomic workflow. Furthermore, 18 individual samples having a mutation in TP53 were assayed by SRM.Two mutant p53 peptides were successfully detected in two cancer cell lines as expected from their DNA sequence. Wild type p53 peptides were detected in both cytosolic pools, however, none of the mutant p53 peptides were identified. Mutations at the protein level were detected in two cytosolic extracts and whole tumor lysates from the same patients by SRM analysis. Six thousand and six hundred and twenty eight non-redundant proteins were identified in the two cytosolic pools, thus greatly improving a previously reported cytosolic proteome.In the current study we show the great potential of using proteogenomics for the direct identification of cancer-associated mutations in clinical samples and we discuss current limitations and future perspectives." @default.
• W2584164846 created "2017-02-10" @default.
• W2584164846 creator A5009510478 @default.
• W2584164846 creator A5035993142 @default.
• W2584164846 creator A5041555565 @default.
• W2584164846 creator A5066680524 @default.
• W2584164846 creator A5081761981 @default.
• W2584164846 creator A5084226800 @default.
• W2584164846 date "2017-01-01" @default.
• W2584164846 modified "2023-10-13" @default.
• W2584164846 title "Variant peptide detection utilizing mass spectrometry: laying the foundations for proteogenomic identification and validation" @default.
• W2584164846 cites W1591934633 @default.
• W2584164846 cites W1973268693 @default.
• W2584164846 cites W1981703479 @default.
• W2584164846 cites W2006960173 @default.
• W2584164846 cites W2009700463 @default.
• W2584164846 cites W2013359618 @default.
• W2584164846 cites W2013800580 @default.
• W2584164846 cites W2014344192 @default.
• W2584164846 cites W2014769842 @default.
• W2584164846 cites W2016590130 @default.
• W2584164846 cites W2026433130 @default.
• W2584164846 cites W2030498712 @default.
• W2584164846 cites W2039997444 @default.
• W2584164846 cites W2042228684 @default.
• W2584164846 cites W2044433401 @default.
• W2584164846 cites W2057683129 @default.
• W2584164846 cites W2059999514 @default.
• W2584164846 cites W2060121473 @default.
• W2584164846 cites W2084286460 @default.
• W2584164846 cites W2091921928 @default.
• W2584164846 cites W2095829177 @default.
• W2584164846 cites W2095857163 @default.
• W2584164846 cites W2096043422 @default.
• W2584164846 cites W2101879802 @default.
• W2584164846 cites W2103304205 @default.
• W2584164846 cites W2103593166 @default.
• W2584164846 cites W2109564400 @default.
• W2584164846 cites W2111703821 @default.
• W2584164846 cites W2114031931 @default.
• W2584164846 cites W2128316695 @default.
• W2584164846 cites W2128381498 @default.
• W2584164846 cites W2128551987 @default.
• W2584164846 cites W2130153722 @default.
• W2584164846 cites W2131856634 @default.
• W2584164846 cites W2155849574 @default.
• W2584164846 cites W2165064122 @default.
• W2584164846 cites W2166262263 @default.
• W2584164846 cites W2168303063 @default.
• W2584164846 cites W2171777347 @default.
• W2584164846 cites W2264342946 @default.
• W2584164846 cites W4252395482 @default.
• W2584164846 doi "https://doi.org/10.1515/cclm-2016-0947" @default.
• W2584164846 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28157690" @default.
• W2584164846 hasPublicationYear "2017" @default.
• W2584164846 type Work @default.
• W2584164846 sameAs 2584164846 @default.
• W2584164846 citedByCount "6" @default.
• W2584164846 countsByYear W25841648462017 @default.
• W2584164846 countsByYear W25841648462018 @default.
• W2584164846 countsByYear W25841648462021 @default.
• W2584164846 countsByYear W25841648462023 @default.
• W2584164846 crossrefType "journal-article" @default.
• W2584164846 hasAuthorship W2584164846A5009510478 @default.
• W2584164846 hasAuthorship W2584164846A5035993142 @default.
• W2584164846 hasAuthorship W2584164846A5041555565 @default.
• W2584164846 hasAuthorship W2584164846A5066680524 @default.
• W2584164846 hasAuthorship W2584164846A5081761981 @default.
• W2584164846 hasAuthorship W2584164846A5084226800 @default.
• W2584164846 hasConcept C104317684 @default.
• W2584164846 hasConcept C104397665 @default.
• W2584164846 hasConcept C141231307 @default.
• W2584164846 hasConcept C143065580 @default.
• W2584164846 hasConcept C145741570 @default.
• W2584164846 hasConcept C153911025 @default.
• W2584164846 hasConcept C189206191 @default.
• W2584164846 hasConcept C2779281246 @default.
• W2584164846 hasConcept C46111723 @default.
• W2584164846 hasConcept C55493867 @default.
• W2584164846 hasConcept C68289359 @default.
• W2584164846 hasConcept C70721500 @default.
• W2584164846 hasConcept C86803240 @default.
• W2584164846 hasConceptScore W2584164846C104317684 @default.
• W2584164846 hasConceptScore W2584164846C104397665 @default.
• W2584164846 hasConceptScore W2584164846C141231307 @default.
• W2584164846 hasConceptScore W2584164846C143065580 @default.
• W2584164846 hasConceptScore W2584164846C145741570 @default.
• W2584164846 hasConceptScore W2584164846C153911025 @default.
• W2584164846 hasConceptScore W2584164846C189206191 @default.
• W2584164846 hasConceptScore W2584164846C2779281246 @default.
• W2584164846 hasConceptScore W2584164846C46111723 @default.
• W2584164846 hasConceptScore W2584164846C55493867 @default.
• W2584164846 hasConceptScore W2584164846C68289359 @default.
• W2584164846 hasConceptScore W2584164846C70721500 @default.
• W2584164846 hasConceptScore W2584164846C86803240 @default.
• W2584164846 hasIssue "9" @default.
• W2584164846 hasLocation W25841648461 @default.
• W2584164846 hasLocation W25841648462 @default.
• W2584164846 hasOpenAccess W2584164846 @default.
• W2584164846 hasPrimaryLocation W25841648461 @default.

• next