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- W2584663945 abstract "Tunable diode laser absorption spectroscopy (TDLAS) was evaluated on its potential to detect bacterial growth of contaminated media fill vials. The target was a replacement/ automation of the traditional visual media fill inspection. TDLAS was used to determine non-invasively O2 and/or CO2 changes in headspaces of such vials being induced by metabolically active microorganisms. Four different vial formats, 34 microorganisms (inoculation volume<10 cells) and two different media (TSB/FTM) were tested. Applying parallel CO2 and O2 headspace measurements all format-organism combinations were detected within <11 days reliably with reproducible results. False negatives were exclusively observed for samples that were intentionally breached with syringes of 0.3 mm in diameter. Overall it was shown that TDLAS functionality for a replacement of the visual media fill inspection is given and that investing in further validation and implementation studies is valuable. Nevertheless, some small but vincible challenges remain to have this technology in practical use." @default.
- W2584663945 created "2017-02-10" @default.
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- W2584663945 date "2017-05-01" @default.
- W2584663945 modified "2023-09-24" @default.
- W2584663945 title "Tunable diode laser absorption spectroscopy as method of choice for non-invasive and automated detection of microbial growth in media fills" @default.
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- W2584663945 doi "https://doi.org/10.1016/j.talanta.2017.01.088" @default.
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