Matches in SemOpenAlex for { <https://semopenalex.org/work/W2585489787> ?p ?o ?g. }
- W2585489787 endingPage "55" @default.
- W2585489787 startingPage "44" @default.
- W2585489787 abstract "Iron-sulfur clusters perform essential functions in enzymatic catalysis and homeostatic regulation. Here we for the first time identified Ssq1 as an essential component for iron-sulfur cluster assembly in Candida albicans. Ssq1 played an important role in cell growth. Shutting off SSQ1 led to accumulation of intracellular iron, especially in mitochondria, and disorder of intracellular iron regulation. In tetO-SSQ1, iron overloading triggered the oxidative damage of mitochondrial function. Surprisingly, disruption of SSQ1 activated autophagic pathway. The mitochondrial dysfunction was further aggravated when CCZ1 (which is essential for autophagy) and SSQ1 was simultaneously deleted, suggesting that autophagy played a critical role in maintenance of mitochondrial function in tetO-SSQ1. In addition, double deletion of SSQ1 and CCZ1 further elevated cellular iron levels in comparison with tetO-SSQ1, indicating that autophagy participated in maintenance of iron homeostasis. Furthermore, we found that loss of SSQ1 led to increasing protein expression of Rnr1 and redistribution of Rnr2 from the nucleus to cytoplasm, and further resulted in cell cycle arrest. The results implied that cell cycle arrest was caused by activating the checkpoint pathway because of impairing the iron-sulfur cluster assembly in tetO-SSQ1. Shutting off SSQ1 led to a significant defect in filamentous development. Interestingly, the tetO-SSQ1ccz1Δ/Δ growth was inhibited on hyphae-inducing solid media. Both tetO-SSQ1 and tetO-SSQ1ccz1Δ/Δ exhibited extremely attenuated virulence, indicating that Ssq1 might provide a promising target for antifungal drugs development. In summary, our findings provide new insights into the understanding of iron-sulfur cluster assembly-related gene in C. albicans." @default.
- W2585489787 created "2017-02-10" @default.
- W2585489787 creator A5002532636 @default.
- W2585489787 creator A5002877635 @default.
- W2585489787 creator A5019945679 @default.
- W2585489787 creator A5025991586 @default.
- W2585489787 creator A5030770038 @default.
- W2585489787 creator A5045958420 @default.
- W2585489787 creator A5047307365 @default.
- W2585489787 creator A5052148101 @default.
- W2585489787 creator A5053320254 @default.
- W2585489787 creator A5055493931 @default.
- W2585489787 creator A5086559201 @default.
- W2585489787 date "2017-04-01" @default.
- W2585489787 modified "2023-10-06" @default.
- W2585489787 title "Loss of Ssq1 leads to mitochondrial dysfunction, activation of autophagy and cell cycle arrest due to iron overload triggered by mitochondrial iron–sulfur cluster assembly defects in Candida albicans" @default.
- W2585489787 cites W1494648262 @default.
- W2585489787 cites W1531120778 @default.
- W2585489787 cites W1581769390 @default.
- W2585489787 cites W1602738992 @default.
- W2585489787 cites W1632467620 @default.
- W2585489787 cites W1683762860 @default.
- W2585489787 cites W1964601437 @default.
- W2585489787 cites W1965618865 @default.
- W2585489787 cites W1965784004 @default.
- W2585489787 cites W1968811439 @default.
- W2585489787 cites W1969743438 @default.
- W2585489787 cites W1975497763 @default.
- W2585489787 cites W1976493565 @default.
- W2585489787 cites W1980667691 @default.
- W2585489787 cites W1982057097 @default.
- W2585489787 cites W1985155016 @default.
- W2585489787 cites W1991149284 @default.
- W2585489787 cites W1991488616 @default.
- W2585489787 cites W1991681466 @default.
- W2585489787 cites W1996407872 @default.
- W2585489787 cites W1999869813 @default.
- W2585489787 cites W2004256141 @default.
- W2585489787 cites W2004367369 @default.
- W2585489787 cites W2007502654 @default.
- W2585489787 cites W2009715420 @default.
- W2585489787 cites W2010802040 @default.
- W2585489787 cites W2011348831 @default.
- W2585489787 cites W2013544053 @default.
- W2585489787 cites W2018411741 @default.
- W2585489787 cites W2023974479 @default.
- W2585489787 cites W2026981180 @default.
- W2585489787 cites W2031212222 @default.
- W2585489787 cites W2035347343 @default.
- W2585489787 cites W2039261716 @default.
- W2585489787 cites W2040338195 @default.
- W2585489787 cites W2045197265 @default.
- W2585489787 cites W2046264363 @default.
- W2585489787 cites W2046812895 @default.
- W2585489787 cites W2048177923 @default.
- W2585489787 cites W2048847466 @default.
- W2585489787 cites W2050828231 @default.
- W2585489787 cites W2052916872 @default.
- W2585489787 cites W2054053117 @default.
- W2585489787 cites W2055353515 @default.
- W2585489787 cites W2058381923 @default.
- W2585489787 cites W2059180477 @default.
- W2585489787 cites W2060711678 @default.
- W2585489787 cites W2068700852 @default.
- W2585489787 cites W2069499122 @default.
- W2585489787 cites W2079889764 @default.
- W2585489787 cites W2085796851 @default.
- W2585489787 cites W2092038519 @default.
- W2585489787 cites W2092046191 @default.
- W2585489787 cites W2093869242 @default.
- W2585489787 cites W2095217602 @default.
- W2585489787 cites W2095294326 @default.
- W2585489787 cites W2095802468 @default.
- W2585489787 cites W2103890028 @default.
- W2585489787 cites W2105455714 @default.
- W2585489787 cites W2105683783 @default.
- W2585489787 cites W2112800403 @default.
- W2585489787 cites W2117003937 @default.
- W2585489787 cites W2119021294 @default.
- W2585489787 cites W2119507799 @default.
- W2585489787 cites W2127645577 @default.
- W2585489787 cites W2128207311 @default.
- W2585489787 cites W2130620383 @default.
- W2585489787 cites W2133685961 @default.
- W2585489787 cites W2136053113 @default.
- W2585489787 cites W2136321020 @default.
- W2585489787 cites W2137209776 @default.
- W2585489787 cites W2144035662 @default.
- W2585489787 cites W2144737284 @default.
- W2585489787 cites W2150543962 @default.
- W2585489787 cites W2162031449 @default.
- W2585489787 cites W2179627510 @default.
- W2585489787 doi "https://doi.org/10.1016/j.biocel.2017.01.021" @default.
- W2585489787 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28163187" @default.
- W2585489787 hasPublicationYear "2017" @default.
- W2585489787 type Work @default.
- W2585489787 sameAs 2585489787 @default.
- W2585489787 citedByCount "19" @default.