FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2585753733> ?p ?o ?g. }

• W2585753733 abstract "The mammalian target of rapamycin (mTOR) is a kinase involved in a variety of physiological and pathological functions. However, the exact role of mTOR in excitotoxicity is poorly understood. Here, we investigated the effects of mTOR inhibition with rapamycin against neurodegeneration, and motor impairment, as well as inflammatory profile caused by an excitotoxic stimulus. A single and unilateral striatal injection of quinolinic acid (QA) was used to induce excitotoxicity in mice. Rapamycin (250 nL of 0.2, 2, or 20 μM; intrastriatal route) was administered 15 min before QA injection. Forty-eight hours after QA administration, rotarod test was performed to evaluate motor coordination and balance. Fluoro-Jade C, Iba-1, and GFAP staining were used to evaluate neuronal cell death, microglia morphology, and astrocytes density, respectively, at this time point. Levels of cytokines and neurotrophic factors were measured by ELISA and Cytometric Bead Array 8 h after QA injection. Striatal synaptosomes were used to evaluate the release of glutamate. We first demonstrated that rapamycin prevented the motor impairment induced by QA. Moreover, mTOR inhibition also reduced the neurodegeneration and the production of interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF)-α induced by excitotoxic stimulus. The lowest dose of rapamycin also increased the production of IL-10 and prevented the reduction of astrocyte density induced by QA. By using an in vitro approach, we demonstrated that rapamycin differently alters the release of glutamate from striatal synaptosomes induced by QA, reducing or enhancing the release of this neurotransmitter at low or high concentrations, respectively. Taken together, these data demonstrated a protective effect of rapamycin against an excitotoxic stimulus. Therefore, this study provides new evidence of the detrimental role of mTOR in neurodegeneration, which might represent an important target for the treatment of neurodegenerative diseases." @default.
• W2585753733 created "2017-02-10" @default.
• W2585753733 creator A5016174155 @default.
• W2585753733 creator A5032503259 @default.
• W2585753733 creator A5039339341 @default.
• W2585753733 creator A5040845311 @default.
• W2585753733 creator A5059221576 @default.
• W2585753733 creator A5064044893 @default.
• W2585753733 creator A5065756562 @default.
• W2585753733 creator A5073191722 @default.
• W2585753733 date "2017-01-31" @default.
• W2585753733 modified "2023-09-27" @default.
• W2585753733 title "Neuroprotective effects of intrastriatal injection of rapamycin in a mouse model of excitotoxicity induced by quinolinic acid" @default.
• W2585753733 cites W1487659381 @default.
• W2585753733 cites W1548964109 @default.
• W2585753733 cites W1942602833 @default.
• W2585753733 cites W1967663704 @default.
• W2585753733 cites W1968962277 @default.
• W2585753733 cites W1978312717 @default.
• W2585753733 cites W1982807202 @default.
• W2585753733 cites W1986442348 @default.
• W2585753733 cites W1987484120 @default.
• W2585753733 cites W1989779456 @default.
• W2585753733 cites W1989882546 @default.
• W2585753733 cites W1995130178 @default.
• W2585753733 cites W1996056108 @default.
• W2585753733 cites W2003337582 @default.
• W2585753733 cites W2003643542 @default.
• W2585753733 cites W2003738638 @default.
• W2585753733 cites W2004156024 @default.
• W2585753733 cites W2007607107 @default.
• W2585753733 cites W2025452473 @default.
• W2585753733 cites W2035934190 @default.
• W2585753733 cites W2049625461 @default.
• W2585753733 cites W2051353156 @default.
• W2585753733 cites W2057019886 @default.
• W2585753733 cites W2057210301 @default.
• W2585753733 cites W2061207071 @default.
• W2585753733 cites W2062497077 @default.
• W2585753733 cites W2063285691 @default.
• W2585753733 cites W2066522177 @default.
• W2585753733 cites W2067943520 @default.
• W2585753733 cites W2070759879 @default.
• W2585753733 cites W2071088136 @default.
• W2585753733 cites W2071193766 @default.
• W2585753733 cites W2074695844 @default.
• W2585753733 cites W2075010970 @default.
• W2585753733 cites W2079627863 @default.
• W2585753733 cites W2082056883 @default.
• W2585753733 cites W2082827804 @default.
• W2585753733 cites W2083949851 @default.
• W2585753733 cites W2111567059 @default.
• W2585753733 cites W2136052079 @default.
• W2585753733 cites W2142630817 @default.
• W2585753733 cites W2143935514 @default.
• W2585753733 cites W2145702440 @default.
• W2585753733 cites W2160239222 @default.
• W2585753733 cites W2164522123 @default.
• W2585753733 cites W2165224523 @default.
• W2585753733 cites W2168714103 @default.
• W2585753733 cites W2168738541 @default.
• W2585753733 cites W2169928434 @default.
• W2585753733 cites W2171668977 @default.
• W2585753733 cites W2173271778 @default.
• W2585753733 cites W2188762618 @default.
• W2585753733 cites W2257271597 @default.
• W2585753733 cites W2276290754 @default.
• W2585753733 cites W2347162009 @default.
• W2585753733 cites W2400280298 @default.
• W2585753733 doi "https://doi.org/10.1186/s12974-017-0793-x" @default.
• W2585753733 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5282622" @default.
• W2585753733 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28143498" @default.
• W2585753733 hasPublicationYear "2017" @default.
• W2585753733 type Work @default.
• W2585753733 sameAs 2585753733 @default.
• W2585753733 citedByCount "17" @default.
• W2585753733 countsByYear W25857537332017 @default.
• W2585753733 countsByYear W25857537332018 @default.
• W2585753733 countsByYear W25857537332019 @default.
• W2585753733 countsByYear W25857537332020 @default.
• W2585753733 countsByYear W25857537332021 @default.
• W2585753733 countsByYear W25857537332022 @default.
• W2585753733 countsByYear W25857537332023 @default.
• W2585753733 crossrefType "journal-article" @default.
• W2585753733 hasAuthorship W2585753733A5016174155 @default.
• W2585753733 hasAuthorship W2585753733A5032503259 @default.
• W2585753733 hasAuthorship W2585753733A5039339341 @default.
• W2585753733 hasAuthorship W2585753733A5040845311 @default.
• W2585753733 hasAuthorship W2585753733A5059221576 @default.
• W2585753733 hasAuthorship W2585753733A5064044893 @default.
• W2585753733 hasAuthorship W2585753733A5065756562 @default.
• W2585753733 hasAuthorship W2585753733A5073191722 @default.
• W2585753733 hasBestOaLocation W25857537331 @default.
• W2585753733 hasConcept C126322002 @default.
• W2585753733 hasConcept C169760540 @default.
• W2585753733 hasConcept C170493617 @default.
• W2585753733 hasConcept C185592680 @default.
• W2585753733 hasConcept C190283241 @default.
• W2585753733 hasConcept C25498285 @default.
• W2585753733 hasConcept C25876315 @default.

• next