Matches in SemOpenAlex for { <https://semopenalex.org/work/W25857564> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W25857564 abstract "Autophagic pathways have been shown to regulate neural precursor cell (NPC) survival during nervous system development. To determine whether lysosomal dysfunction leads to increased autophagic vacuoles (AVs) and NPC death, we treated mouse cerebellar neural stem cells (C17.2 cell line) and FGF2-expanded primary cerebellar NPCs with lysosomal dysfunction inducing agents chloroquine (CQ), bafilomycin A1 (Baf A1), and ammonium chloride (NH4Cl). CQ, NH4Cl, and Baf A1 treated NPCs underwent apoptosis, as evidenced by cytological features and cleaved caspase-3 immunoreactivity. In comparison to wild-type NPCs, Bax-deficient NPCs exhibited significantly less caspase-3 activation and cell death in response to lysosomal dysfunction inducing agents. These agents also produced increased levels of autophagy-associated proteins LC3-II and Beclin1. Cycloheximide, a protein synthesis inhibitor, attenuated AV formation in response to CQ as evidenced by decreased LC3-II. However, cycloheximide had no effect on caspase-3 activation or cell death indicating that AV accumulation is not necessary for CQ-induced NPC death. p53-deficient NPCs showed significant protection from CQ-induced death compared to wild-type NPCs. In total, our data demonstrate that lysosomal dysfunction leads to AV accumulation as well as p53- and Bax- dependent NPC death." @default.
- W25857564 created "2016-06-24" @default.
- W25857564 creator A5003163550 @default.
- W25857564 creator A5004894610 @default.
- W25857564 creator A5045330493 @default.
- W25857564 creator A5067993987 @default.
- W25857564 date "2008-03-01" @default.
- W25857564 modified "2023-09-25" @default.
- W25857564 title "Lysosomal Dysfunction Promotes Autophagic Stress and NPC Death" @default.
- W25857564 doi "https://doi.org/10.1096/fasebj.22.1_supplement.1121.10" @default.
- W25857564 hasPublicationYear "2008" @default.
- W25857564 type Work @default.
- W25857564 sameAs 25857564 @default.
- W25857564 citedByCount "0" @default.
- W25857564 crossrefType "journal-article" @default.
- W25857564 hasAuthorship W25857564A5003163550 @default.
- W25857564 hasAuthorship W25857564A5004894610 @default.
- W25857564 hasAuthorship W25857564A5045330493 @default.
- W25857564 hasAuthorship W25857564A5067993987 @default.
- W25857564 hasConcept C102568950 @default.
- W25857564 hasConcept C185592680 @default.
- W25857564 hasConcept C190062978 @default.
- W25857564 hasConcept C190283241 @default.
- W25857564 hasConcept C203522944 @default.
- W25857564 hasConcept C2777722557 @default.
- W25857564 hasConcept C2778128677 @default.
- W25857564 hasConcept C31573885 @default.
- W25857564 hasConcept C502942594 @default.
- W25857564 hasConcept C54355233 @default.
- W25857564 hasConcept C55493867 @default.
- W25857564 hasConcept C81885089 @default.
- W25857564 hasConcept C86803240 @default.
- W25857564 hasConcept C95444343 @default.
- W25857564 hasConcept C98424977 @default.
- W25857564 hasConceptScore W25857564C102568950 @default.
- W25857564 hasConceptScore W25857564C185592680 @default.
- W25857564 hasConceptScore W25857564C190062978 @default.
- W25857564 hasConceptScore W25857564C190283241 @default.
- W25857564 hasConceptScore W25857564C203522944 @default.
- W25857564 hasConceptScore W25857564C2777722557 @default.
- W25857564 hasConceptScore W25857564C2778128677 @default.
- W25857564 hasConceptScore W25857564C31573885 @default.
- W25857564 hasConceptScore W25857564C502942594 @default.
- W25857564 hasConceptScore W25857564C54355233 @default.
- W25857564 hasConceptScore W25857564C55493867 @default.
- W25857564 hasConceptScore W25857564C81885089 @default.
- W25857564 hasConceptScore W25857564C86803240 @default.
- W25857564 hasConceptScore W25857564C95444343 @default.
- W25857564 hasConceptScore W25857564C98424977 @default.
- W25857564 hasIssue "S1" @default.
- W25857564 hasLocation W258575641 @default.
- W25857564 hasOpenAccess W25857564 @default.
- W25857564 hasPrimaryLocation W258575641 @default.
- W25857564 hasRelatedWork W1506592783 @default.
- W25857564 hasRelatedWork W1987695408 @default.
- W25857564 hasRelatedWork W2006416158 @default.
- W25857564 hasRelatedWork W2027482850 @default.
- W25857564 hasRelatedWork W2040546382 @default.
- W25857564 hasRelatedWork W2097345042 @default.
- W25857564 hasRelatedWork W2156797519 @default.
- W25857564 hasRelatedWork W2276245786 @default.
- W25857564 hasRelatedWork W2322187697 @default.
- W25857564 hasRelatedWork W2612315300 @default.
- W25857564 hasVolume "22" @default.
- W25857564 isParatext "false" @default.
- W25857564 isRetracted "false" @default.
- W25857564 magId "25857564" @default.
- W25857564 workType "article" @default.