FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2585774534> ?p ?o ?g. }

• W2585774534 endingPage "4788" @default.
• W2585774534 startingPage "4777" @default.
• W2585774534 abstract "During DNA replication in eukaryotic cells, short single-stranded DNA segments known as Okazaki fragments are first synthesized on the lagging strand. The Okazaki fragments originate from ∼35-nucleotide-long RNA-DNA primers. After Okazaki fragment synthesis, these primers must be removed to allow fragment joining into a continuous lagging strand. To date, the models of enzymatic machinery that removes the RNA-DNA primers have come almost exclusively from biochemical reconstitution studies and some genetic interaction assays, and there is little direct evidence to confirm these models. One obstacle to elucidating Okazaki fragment processing has been the lack of methods that can directly examine primer removal in vivo. In this study, we developed an electron microscopy assay that can visualize nucleotide flap structures on DNA replication forks in fission yeast (Schizosaccharomyces pombe). With this assay, we first demonstrated the generation of flap structures during Okazaki fragment processing in vivo. The mean and median lengths of the flaps in wild-type cells were ∼51 and ∼41 nucleotides, respectively. We also used yeast mutants to investigate the impact of deleting key DNA replication nucleases on these flap structures. Our results provided direct in vivo evidence for a previously proposed flap cleavage pathway and the critical function of Dna2 and Fen1 in cleaving these flaps. In addition, we found evidence for another previously proposed exonucleolytic pathway involving RNA-DNA primer digestion by exonucleases RNase H2 and Exo1. Taken together, our observations suggest a dual mechanism for Okazaki fragment maturation in lagging strand synthesis and establish a new strategy for interrogation of this fascinating process." @default.
• W2585774534 created "2017-02-10" @default.
• W2585774534 creator A5037205520 @default.
• W2585774534 creator A5064661344 @default.
• W2585774534 creator A5071331914 @default.
• W2585774534 creator A5090192314 @default.
• W2585774534 date "2017-03-01" @default.
• W2585774534 modified "2023-10-13" @default.
• W2585774534 title "Direct Visualization of RNA-DNA Primer Removal from Okazaki Fragments Provides Support for Flap Cleavage and Exonucleolytic Pathways in Eukaryotic Cells" @default.
• W2585774534 cites W1490159978 @default.
• W2585774534 cites W1506492200 @default.
• W2585774534 cites W1508748054 @default.
• W2585774534 cites W1516514485 @default.
• W2585774534 cites W1559981481 @default.
• W2585774534 cites W1599690136 @default.
• W2585774534 cites W1729887059 @default.
• W2585774534 cites W1774053018 @default.
• W2585774534 cites W1966219094 @default.
• W2585774534 cites W1967209251 @default.
• W2585774534 cites W1970184264 @default.
• W2585774534 cites W1972653649 @default.
• W2585774534 cites W1986113777 @default.
• W2585774534 cites W1992210568 @default.
• W2585774534 cites W1994245744 @default.
• W2585774534 cites W1997002747 @default.
• W2585774534 cites W2037845691 @default.
• W2585774534 cites W2045906945 @default.
• W2585774534 cites W2046066638 @default.
• W2585774534 cites W2053213954 @default.
• W2585774534 cites W2053712479 @default.
• W2585774534 cites W2054842101 @default.
• W2585774534 cites W2055348937 @default.
• W2585774534 cites W2059184834 @default.
• W2585774534 cites W2061754995 @default.
• W2585774534 cites W2063771471 @default.
• W2585774534 cites W2064264662 @default.
• W2585774534 cites W2067343931 @default.
• W2585774534 cites W2068309168 @default.
• W2585774534 cites W2081383594 @default.
• W2585774534 cites W2082175154 @default.
• W2585774534 cites W2082478355 @default.
• W2585774534 cites W2089594949 @default.
• W2585774534 cites W2090110799 @default.
• W2585774534 cites W2104261681 @default.
• W2585774534 cites W2107856790 @default.
• W2585774534 cites W2110161513 @default.
• W2585774534 cites W2111202021 @default.
• W2585774534 cites W2111313204 @default.
• W2585774534 cites W2117423564 @default.
• W2585774534 cites W2129849452 @default.
• W2585774534 cites W2133231925 @default.
• W2585774534 cites W2134832431 @default.
• W2585774534 cites W2138055292 @default.
• W2585774534 cites W2139039510 @default.
• W2585774534 cites W2146851796 @default.
• W2585774534 cites W2148316521 @default.
• W2585774534 cites W2150867189 @default.
• W2585774534 cites W2157435389 @default.
• W2585774534 cites W2165336005 @default.
• W2585774534 cites W2166672594 @default.
• W2585774534 cites W2332717883 @default.
• W2585774534 doi "https://doi.org/10.1074/jbc.m116.758599" @default.
• W2585774534 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5377794" @default.
• W2585774534 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28159842" @default.
• W2585774534 hasPublicationYear "2017" @default.
• W2585774534 type Work @default.
• W2585774534 sameAs 2585774534 @default.
• W2585774534 citedByCount "40" @default.
• W2585774534 countsByYear W25857745342017 @default.
• W2585774534 countsByYear W25857745342018 @default.
• W2585774534 countsByYear W25857745342019 @default.
• W2585774534 countsByYear W25857745342020 @default.
• W2585774534 countsByYear W25857745342021 @default.
• W2585774534 countsByYear W25857745342022 @default.
• W2585774534 countsByYear W25857745342023 @default.
• W2585774534 crossrefType "journal-article" @default.
• W2585774534 hasAuthorship W2585774534A5037205520 @default.
• W2585774534 hasAuthorship W2585774534A5064661344 @default.
• W2585774534 hasAuthorship W2585774534A5071331914 @default.
• W2585774534 hasAuthorship W2585774534A5090192314 @default.
• W2585774534 hasBestOaLocation W25857745341 @default.
• W2585774534 hasConcept C104317684 @default.
• W2585774534 hasConcept C11305352 @default.
• W2585774534 hasConcept C151730666 @default.
• W2585774534 hasConcept C153911025 @default.
• W2585774534 hasConcept C175156509 @default.
• W2585774534 hasConcept C178790620 @default.
• W2585774534 hasConcept C185592680 @default.
• W2585774534 hasConcept C2777563447 @default.
• W2585774534 hasConcept C43369102 @default.
• W2585774534 hasConcept C552990157 @default.
• W2585774534 hasConcept C55493867 @default.
• W2585774534 hasConcept C67705224 @default.
• W2585774534 hasConcept C73573662 @default.
• W2585774534 hasConcept C86803240 @default.
• W2585774534 hasConcept C95444343 @default.
• W2585774534 hasConcept C98086919 @default.
• W2585774534 hasConceptScore W2585774534C104317684 @default.

• next