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- W2585957820 abstract "A supersensitive nanoimmunosensor of cancer antigen 125 (CA125), which is a stage marker of several types of cancers, was developed based on characterization of single-particle plasmonic scattering by wavelength-dependent enhanced dark-field microscopy with dual-detection mode (EDFM-DM). Considering the optical properties of gold and silver nanoparticles (AuNPs/AgNPs), 40-nm AgNP was selected as an optimal fluorescence-free probe. CA125 was analyzed using the wavelength-dependent plasmonic scattering intensity of 40-nm AgNP-labeled CA125 immunoreacted on gold nanodots under the dual-detection mode. A color digital camera and an electron multiplying charge-coupled device camera were used for qualitative and quantitative analysis, respectively, based on the dark-field scattering images. Under optimal conditions, the developed immunosensor exhibited a lower detection limit of 4 μU/mL (S/N = 3) with a wide dynamic detection range of 4 μU/mL-80 U/mL (R = 0.9935), which was a 100–375,000-fold lower detection limit with a 100–100,000-fold wider dynamic range than previous methods. In addition, recovery was greater than 98% with the spiking of standard CA125 in human serum samples. This method may be an excellent nanoimmunoassay platform for supersensitive detection at a wide dynamic detection range, supporting the earliest-stage detection of various disease-related protein molecules at the single-molecule level." @default.
- W2585957820 created "2017-02-10" @default.
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- W2585957820 date "2017-06-01" @default.
- W2585957820 modified "2023-10-03" @default.
- W2585957820 title "Supersensitive single-particle plasmonic scattering-based cancer antigen 125 nanoimmunosensor by enhanced dark-field microscopy with dual-detection mode" @default.
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- W2585957820 doi "https://doi.org/10.1016/j.snb.2017.01.191" @default.
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