Matches in SemOpenAlex for { <https://semopenalex.org/work/W2586587438> ?p ?o ?g. }
- W2586587438 endingPage "e0170290" @default.
- W2586587438 startingPage "e0170290" @default.
- W2586587438 abstract "A simple and reliable DNA extraction of hepatitis B virus (HBV) is critical in developing an ultrasensitive detection method for HBV infection. Current commercially available serum Hepatitis B Virus (HBV) DNA extraction methods are time-consuming, expensive and/or require specialized equipment, which hinders wide adoption of clinical laboratories. This study offers a report on an ultrasensitive HBV DNA detection method by coupling serum HBV DNA extraction by ultrafiltration (UF) with real-time PCR (qPCR) detection.Serum proteins were precipitated by phenol to release HBV DNA in the supernatant which was then transferred to the UF devices. The resultant DNA concentrate was eluted and released into qPCR pre-mixture. The UF-qPCR assay performance, including recovery rate, linearity, detection sensitivity, precision and diagnostic accuracy that compared to the CAP-CTM V2.0 assay by analyzing batched low viral load clinical samples was evaluated.The recovery rate of the UF-based HBV DNA extraction method was above 80%. The assay linearity was demonstrated with a slope of 0.95 and R2 values of 0.99. Limit-of-detection (LOD) of the UF-qPCR assay was determined to be 12.1IU/ml. The coefficient of variation (CV) of HBV quantitation for high, low and limit titer samples was 2.28%, 5.77% and 25.59%, respectively. Accuracy of the UF-qPCR assay was confirmed with the reference panel, and there was a strong correlation between these two methods (R2 = 0.55, p < 0.01).The UF-qPCR assay is reliable, highly sensitive, affordable and time-saving, and the method can be used for ultrasensitive detection of serum HBV." @default.
- W2586587438 created "2017-02-17" @default.
- W2586587438 creator A5010630057 @default.
- W2586587438 creator A5018492075 @default.
- W2586587438 creator A5020002561 @default.
- W2586587438 creator A5034410753 @default.
- W2586587438 creator A5035503058 @default.
- W2586587438 creator A5050199206 @default.
- W2586587438 creator A5052140744 @default.
- W2586587438 creator A5053528169 @default.
- W2586587438 creator A5065544354 @default.
- W2586587438 creator A5081510085 @default.
- W2586587438 date "2017-02-09" @default.
- W2586587438 modified "2023-09-29" @default.
- W2586587438 title "Ultrasensitive detection of serum hepatitis B virus by coupling ultrafiltration DNA extraction with real-time PCR" @default.
- W2586587438 cites W1414498276 @default.
- W2586587438 cites W1500215579 @default.
- W2586587438 cites W1747774310 @default.
- W2586587438 cites W1969036863 @default.
- W2586587438 cites W1971329145 @default.
- W2586587438 cites W1977538965 @default.
- W2586587438 cites W1990516284 @default.
- W2586587438 cites W2004100607 @default.
- W2586587438 cites W2009590060 @default.
- W2586587438 cites W2013850725 @default.
- W2586587438 cites W2017695017 @default.
- W2586587438 cites W2051216879 @default.
- W2586587438 cites W2059597738 @default.
- W2586587438 cites W2070427985 @default.
- W2586587438 cites W2071267501 @default.
- W2586587438 cites W2075861708 @default.
- W2586587438 cites W2078224472 @default.
- W2586587438 cites W2081791504 @default.
- W2586587438 cites W2085988525 @default.
- W2586587438 cites W2094182055 @default.
- W2586587438 cites W2095989309 @default.
- W2586587438 cites W2096669666 @default.
- W2586587438 cites W2111164816 @default.
- W2586587438 cites W2121540238 @default.
- W2586587438 cites W2121599156 @default.
- W2586587438 cites W2129456084 @default.
- W2586587438 cites W2132681792 @default.
- W2586587438 cites W2135976950 @default.
- W2586587438 cites W2138592666 @default.
- W2586587438 cites W2140662961 @default.
- W2586587438 cites W2147283902 @default.
- W2586587438 cites W2148866075 @default.
- W2586587438 cites W2154664891 @default.
- W2586587438 cites W2156015715 @default.
- W2586587438 cites W2410181593 @default.
- W2586587438 cites W2915810710 @default.
- W2586587438 cites W37224905 @default.
- W2586587438 cites W4211026375 @default.
- W2586587438 doi "https://doi.org/10.1371/journal.pone.0170290" @default.
- W2586587438 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5300171" @default.
- W2586587438 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28182626" @default.
- W2586587438 hasPublicationYear "2017" @default.
- W2586587438 type Work @default.
- W2586587438 sameAs 2586587438 @default.
- W2586587438 citedByCount "6" @default.
- W2586587438 countsByYear W25865874382019 @default.
- W2586587438 countsByYear W25865874382020 @default.
- W2586587438 countsByYear W25865874382022 @default.
- W2586587438 countsByYear W25865874382023 @default.
- W2586587438 crossrefType "journal-article" @default.
- W2586587438 hasAuthorship W2586587438A5010630057 @default.
- W2586587438 hasAuthorship W2586587438A5018492075 @default.
- W2586587438 hasAuthorship W2586587438A5020002561 @default.
- W2586587438 hasAuthorship W2586587438A5034410753 @default.
- W2586587438 hasAuthorship W2586587438A5035503058 @default.
- W2586587438 hasAuthorship W2586587438A5050199206 @default.
- W2586587438 hasAuthorship W2586587438A5052140744 @default.
- W2586587438 hasAuthorship W2586587438A5053528169 @default.
- W2586587438 hasAuthorship W2586587438A5065544354 @default.
- W2586587438 hasAuthorship W2586587438A5081510085 @default.
- W2586587438 hasBestOaLocation W25865874381 @default.
- W2586587438 hasConcept C104317684 @default.
- W2586587438 hasConcept C119128265 @default.
- W2586587438 hasConcept C153911025 @default.
- W2586587438 hasConcept C159047783 @default.
- W2586587438 hasConcept C185592680 @default.
- W2586587438 hasConcept C2522874641 @default.
- W2586587438 hasConcept C2780593183 @default.
- W2586587438 hasConcept C32611913 @default.
- W2586587438 hasConcept C43617362 @default.
- W2586587438 hasConcept C4725764 @default.
- W2586587438 hasConcept C48023723 @default.
- W2586587438 hasConcept C49105822 @default.
- W2586587438 hasConcept C50817676 @default.
- W2586587438 hasConcept C552990157 @default.
- W2586587438 hasConcept C55493867 @default.
- W2586587438 hasConcept C78063203 @default.
- W2586587438 hasConcept C86803240 @default.
- W2586587438 hasConcept C89838059 @default.
- W2586587438 hasConceptScore W2586587438C104317684 @default.
- W2586587438 hasConceptScore W2586587438C119128265 @default.
- W2586587438 hasConceptScore W2586587438C153911025 @default.
- W2586587438 hasConceptScore W2586587438C159047783 @default.