Matches in SemOpenAlex for { <https://semopenalex.org/work/W2586760573> ?p ?o ?g. }
Showing items 1 to 82 of
82
with 100 items per page.
- W2586760573 abstract "Fetuin-A (FetA) regulates calcium and phosphate homeostasis. It is also an agonist to toll-like receptor 4 (TLR4) and is related to insulin resistance and inflammation. FetA has also been associated with endothelial dysfunction, which is regulated by oxidative stress. Mechanisms whereby FetA influences vascular function are unknown. We hypothesized that FetA through TLR4 and ROS production induces vascular dysfunction. Mesenteric arteries and vascular cells from WKY rats were studied. Vascular function was analysed by wire myography in the presence or absence of FetA (50 ng/mL) and/or CLI095 (CLI - 10-6M - TLR4 inhibitor). Levels of reactive oxygen species (ROS) were measured by chemiluminescence, Amplex Red (H2O2) and ELISA (nitrotyrosine) Protein oxidation and levels were measured by immunoblotting. WKY vessels exposed to FetA were less sensitive to acetylcholine (Ach)-induced and sodium nitroprusside (SNP)-induced relaxation, while sensitivity to phenylephrine was increased by FetA; an effect blocked by N-acetylcysteine (antioxidant) and ML171 (Nox1 inhibitor). Inhibition of TLR4 blocked FetA effects on endothelial-dependent relaxation and contraction, but not on endothelial-independent relaxation. FetA increased ROS production (131±49.2%), but decreased H2O2 intracellular levels (63±14%) in endothelial cells (EC) (vs. veh, p<0.05); an effect blocked by CLI095. ROS production (66±12.2%), as well as, H2O2 (45±8%) and ONOO- (105±31.6%) levels, were increased by FetA in VSMCs (vs. veh, p<0.05). Protein oxidation was increased by FetA in VSMCs (103±26% vs. veh, p<0.05). In EC, eNOS inactivation (136±38%) and JNK activation (84±5%) were increased by FetA (vs. veh, p<0.05). In VSMCs, Rho kinase activity was increased (200±25% vs. veh, p<0.05) at 30 min; while myosin light chain (MLC) activation was only increased (25±3.56% vs. veh, p<0.05) at 15 min. In summary, FetA influences vascular function through Nox1-ROS dependent mechanisms. FetA-induced endothelial dysfunction and contractile responses involve TLR4. Our findings identify a novel system whereby FetA differentially influences vascular function through Nox1-ROS and TLR4. Vascular responses to FetA may depend on the specific pathway activated." @default.
- W2586760573 created "2017-02-17" @default.
- W2586760573 creator A5009703758 @default.
- W2586760573 creator A5020577597 @default.
- W2586760573 creator A5026573737 @default.
- W2586760573 creator A5028678798 @default.
- W2586760573 creator A5040490939 @default.
- W2586760573 creator A5040521316 @default.
- W2586760573 creator A5065770496 @default.
- W2586760573 creator A5074543598 @default.
- W2586760573 creator A5081248469 @default.
- W2586760573 date "2015-09-01" @default.
- W2586760573 modified "2023-09-25" @default.
- W2586760573 title "Abstract 099: Fetuin-A and Toll-like Receptor 4 Regulate Vascular Function: Role of Nox1" @default.
- W2586760573 doi "https://doi.org/10.1161/hyp.66.suppl_1.099" @default.
- W2586760573 hasPublicationYear "2015" @default.
- W2586760573 type Work @default.
- W2586760573 sameAs 2586760573 @default.
- W2586760573 citedByCount "0" @default.
- W2586760573 crossrefType "journal-article" @default.
- W2586760573 hasAuthorship W2586760573A5009703758 @default.
- W2586760573 hasAuthorship W2586760573A5020577597 @default.
- W2586760573 hasAuthorship W2586760573A5026573737 @default.
- W2586760573 hasAuthorship W2586760573A5028678798 @default.
- W2586760573 hasAuthorship W2586760573A5040490939 @default.
- W2586760573 hasAuthorship W2586760573A5040521316 @default.
- W2586760573 hasAuthorship W2586760573A5065770496 @default.
- W2586760573 hasAuthorship W2586760573A5074543598 @default.
- W2586760573 hasAuthorship W2586760573A5081248469 @default.
- W2586760573 hasConcept C126322002 @default.
- W2586760573 hasConcept C134018914 @default.
- W2586760573 hasConcept C185592680 @default.
- W2586760573 hasConcept C2776061955 @default.
- W2586760573 hasConcept C2776151105 @default.
- W2586760573 hasConcept C2777209548 @default.
- W2586760573 hasConcept C2779719074 @default.
- W2586760573 hasConcept C2780972559 @default.
- W2586760573 hasConcept C48349386 @default.
- W2586760573 hasConcept C55493867 @default.
- W2586760573 hasConcept C71924100 @default.
- W2586760573 hasConcept C86803240 @default.
- W2586760573 hasConceptScore W2586760573C126322002 @default.
- W2586760573 hasConceptScore W2586760573C134018914 @default.
- W2586760573 hasConceptScore W2586760573C185592680 @default.
- W2586760573 hasConceptScore W2586760573C2776061955 @default.
- W2586760573 hasConceptScore W2586760573C2776151105 @default.
- W2586760573 hasConceptScore W2586760573C2777209548 @default.
- W2586760573 hasConceptScore W2586760573C2779719074 @default.
- W2586760573 hasConceptScore W2586760573C2780972559 @default.
- W2586760573 hasConceptScore W2586760573C48349386 @default.
- W2586760573 hasConceptScore W2586760573C55493867 @default.
- W2586760573 hasConceptScore W2586760573C71924100 @default.
- W2586760573 hasConceptScore W2586760573C86803240 @default.
- W2586760573 hasIssue "suppl_1" @default.
- W2586760573 hasLocation W25867605731 @default.
- W2586760573 hasOpenAccess W2586760573 @default.
- W2586760573 hasPrimaryLocation W25867605731 @default.
- W2586760573 hasRelatedWork W1974219489 @default.
- W2586760573 hasRelatedWork W1985140896 @default.
- W2586760573 hasRelatedWork W1988165061 @default.
- W2586760573 hasRelatedWork W2049950696 @default.
- W2586760573 hasRelatedWork W2056374731 @default.
- W2586760573 hasRelatedWork W2152531256 @default.
- W2586760573 hasRelatedWork W2338261812 @default.
- W2586760573 hasRelatedWork W2344971590 @default.
- W2586760573 hasRelatedWork W2597228361 @default.
- W2586760573 hasRelatedWork W2611571384 @default.
- W2586760573 hasRelatedWork W2616466497 @default.
- W2586760573 hasRelatedWork W2620687707 @default.
- W2586760573 hasRelatedWork W2623056905 @default.
- W2586760573 hasRelatedWork W2737427193 @default.
- W2586760573 hasRelatedWork W2737660154 @default.
- W2586760573 hasRelatedWork W2737810796 @default.
- W2586760573 hasRelatedWork W2742220560 @default.
- W2586760573 hasRelatedWork W2766375586 @default.
- W2586760573 hasRelatedWork W2770671976 @default.
- W2586760573 hasRelatedWork W2918991224 @default.
- W2586760573 hasVolume "66" @default.
- W2586760573 isParatext "false" @default.
- W2586760573 isRetracted "false" @default.
- W2586760573 magId "2586760573" @default.
- W2586760573 workType "article" @default.