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• W2587137789 abstract "DNA replication is an essential process for all living organisms, and errors in this process can lead to genetic mutations and disease. An assembly of protein machinery, termed the replisome, coordinates enzymatic activities at the replication fork. The DNA helicase is the heart of the replisome unwinding double-strand DNA at the head of the progressing replisome and providing single-strand templates for DNA polymerases. Replicative helicases are composed of six subunits, and arranged in a ring-like structure where ATP hydrolysis events provide the energy to translocate upon and unwind the DNA. The mechanism of helicase unwinding has been widely studied, but there are still many aspects that remain unknown. It is generally thought that these helicases encircle one strand of DNA while the other is excluded from the central channel of the helicase. Our lab has previously identified an interaction between the excluded strand and the helicase exterior that was important for unwinding in the archaeal MCM helicase. The steric exclusion model of replicative helicase unwinding was expanded to include the excluded strand interactions in this newly proposed steric exclusion and wrapping (SEW) model. Here, we present work that expands on the SEW model by revealing that the bacterial DnaB and mitochondrial Twinkle replicative helicases also interact with the excluded strand. We have also developed a new single-molecule FRET analysis program to characterize these excluded strand interactions. Although the excluded strand interaction is seen in multiple replicative helicases, we propose distinct roles for the interaction based on functional assays and known differences in replisome architecture across the various organisms. We have also begun to characterize the helicase-excluded strand wrapping interaction in the presence of other replisome components, namely the single-strand binding (SSB) protein. We further characterized the archaeal SSB protein from Sulfolobus solfataricus and provide evidence for a novel DNA-helicase-SSB ternary complex. Overall, this thesis makes significant contributions to the understanding of replicative helicase unwinding mechanisms by expanding upon the current steric exclusion and wrapping model and introduces a novel single-molecule FRET analysis program that we anticipate will be adopted and utilized by others in the field." @default.
• W2587137789 created "2017-02-17" @default.
• W2587137789 creator A5068203539 @default.
• W2587137789 date "2016-09-25" @default.
• W2587137789 modified "2023-09-27" @default.
• W2587137789 title "Characterizing Excluded Strand DNA Interactions with Hexameric Helicases and Determining Roles in Unwinding Mechanisms" @default.
• W2587137789 cites W124504601 @default.
• W2587137789 cites W1484779991 @default.
• W2587137789 cites W1501523301 @default.
• W2587137789 cites W1501619406 @default.
• W2587137789 cites W1503858891 @default.
• W2587137789 cites W1512516275 @default.
• W2587137789 cites W1516321864 @default.
• W2587137789 cites W15197759 @default.
• W2587137789 cites W1528821242 @default.
• W2587137789 cites W1531287894 @default.
• W2587137789 cites W1538824305 @default.
• W2587137789 cites W1550760853 @default.
• W2587137789 cites W1560819028 @default.
• W2587137789 cites W1563567580 @default.
• W2587137789 cites W1572086689 @default.
• W2587137789 cites W1576279956 @default.
• W2587137789 cites W1584920009 @default.
• W2587137789 cites W1600484978 @default.
• W2587137789 cites W1606524510 @default.
• W2587137789 cites W160692353 @default.
• W2587137789 cites W1725095175 @default.
• W2587137789 cites W1753199320 @default.
• W2587137789 cites W184803170 @default.
• W2587137789 cites W1964572413 @default.
• W2587137789 cites W1964790454 @default.
• W2587137789 cites W1966156603 @default.
• W2587137789 cites W196616756 @default.
• W2587137789 cites W1966308748 @default.
• W2587137789 cites W1966427711 @default.
• W2587137789 cites W1967784067 @default.
• W2587137789 cites W1969155303 @default.
• W2587137789 cites W1970449119 @default.
• W2587137789 cites W1970843450 @default.
• W2587137789 cites W1970885300 @default.
• W2587137789 cites W1970985606 @default.
• W2587137789 cites W1972381178 @default.
• W2587137789 cites W1972480564 @default.
• W2587137789 cites W1973535211 @default.
• W2587137789 cites W1974563774 @default.
• W2587137789 cites W1975699037 @default.
• W2587137789 cites W1977109739 @default.
• W2587137789 cites W1980955641 @default.
• W2587137789 cites W1984812591 @default.
• W2587137789 cites W1985491895 @default.
• W2587137789 cites W1986088879 @default.
• W2587137789 cites W1986881948 @default.
• W2587137789 cites W1987469328 @default.
• W2587137789 cites W1988037647 @default.
• W2587137789 cites W1988268788 @default.
• W2587137789 cites W1989396934 @default.
• W2587137789 cites W1993968187 @default.
• W2587137789 cites W1993978787 @default.
• W2587137789 cites W1994745779 @default.
• W2587137789 cites W1995136541 @default.
• W2587137789 cites W1995420315 @default.
• W2587137789 cites W1996851263 @default.
• W2587137789 cites W1998280118 @default.
• W2587137789 cites W1998790181 @default.
• W2587137789 cites W1999078233 @default.
• W2587137789 cites W1999925341 @default.
• W2587137789 cites W2000410597 @default.
• W2587137789 cites W2000581423 @default.
• W2587137789 cites W2003336032 @default.
• W2587137789 cites W2003660437 @default.
• W2587137789 cites W2004099247 @default.
• W2587137789 cites W2005402849 @default.
• W2587137789 cites W2006103202 @default.
• W2587137789 cites W2006821707 @default.
• W2587137789 cites W2007278972 @default.
• W2587137789 cites W2008424177 @default.
• W2587137789 cites W2009498355 @default.
• W2587137789 cites W2010676078 @default.
• W2587137789 cites W2011937717 @default.
• W2587137789 cites W2012230395 @default.
• W2587137789 cites W2013833462 @default.
• W2587137789 cites W2015754725 @default.
• W2587137789 cites W2016789453 @default.
• W2587137789 cites W2017127381 @default.
• W2587137789 cites W2017390142 @default.
• W2587137789 cites W2017551066 @default.
• W2587137789 cites W2018982396 @default.
• W2587137789 cites W2024429438 @default.
• W2587137789 cites W2024665319 @default.
• W2587137789 cites W2026933728 @default.
• W2587137789 cites W2027513549 @default.
• W2587137789 cites W2031000298 @default.
• W2587137789 cites W2032554048 @default.
• W2587137789 cites W2034902378 @default.
• W2587137789 cites W2037602734 @default.
• W2587137789 cites W2038994322 @default.
• W2587137789 cites W2039857324 @default.
• W2587137789 cites W2040390500 @default.
• W2587137789 cites W2041245869 @default.
• W2587137789 cites W2043836017 @default.

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