Matches in SemOpenAlex for { <https://semopenalex.org/work/W2587479669> ?p ?o ?g. }
Showing items 1 to 65 of
65
with 100 items per page.
- W2587479669 abstract "Although sulfite is widely used to counteract enzymatic browning, its mechanism has remained largely unknown. We describe a double inhibitory mechanism of sulfite on enzymatic browning, affecting both the enzymatic oxidation of phenols into o‑quinones, as well as the non‑enzymatic reactions of these o‑quinones into brown pigments. The non‑enzymatic step is inhibited by formation of addition products of sulfite and o‑quinones, sulfophenolics. Sulfonated derivatives of chlorogenic acid were found in sulfite‑treated potato juice, and their structure was confirmed by mass spectrometry and nuclear magnetic resonance spectroscopy. Sulfonation of chlorogenic acid was demonstrated to occur via tyrosinase‑catalyzed o‑quinone formation. Tyrosinase activity was also irreversibly inactivated, in a relative slow time‑dependent way. Simultaneous treatment of tyrosinase with sulfite and competitive inhibitors of tyrosinase did not result in irreversible inactivation, indicating that sulfite acts in the active site of tyrosinase. LC‑MS analysis of protease digests of sulfite‑treated tyrosinase indicated that inactivation occurred via covalent modification of a single amino acid residue in the active site, most likely a copper‑coordinating histidine residue, which is conserved in all PPOs. As the use of sulfite is controversial, we investigated the effect of potential natural inhibitors of enzymatic browning. Two different polyphenol oxidases (PPOs) were used to screen 60 plant extracts for potential inhibitors. PPOs were found to respond differently to these extracts: an extract that inhibited one PPO could activate the other. This suggests that natural alternatives to replace more generic anti‑browning agents, such as sulfite, are PPO specific." @default.
- W2587479669 created "2017-02-17" @default.
- W2587479669 creator A5005476379 @default.
- W2587479669 creator A5016397696 @default.
- W2587479669 date "2013-01-01" @default.
- W2587479669 modified "2023-09-27" @default.
- W2587479669 title "Inhibition of tyrosinase-mediated enzymatic browning by sulfite and natural alternatives" @default.
- W2587479669 hasPublicationYear "2013" @default.
- W2587479669 type Work @default.
- W2587479669 sameAs 2587479669 @default.
- W2587479669 citedByCount "0" @default.
- W2587479669 crossrefType "journal-article" @default.
- W2587479669 hasAuthorship W2587479669A5005476379 @default.
- W2587479669 hasAuthorship W2587479669A5016397696 @default.
- W2587479669 hasConcept C178790620 @default.
- W2587479669 hasConcept C181199279 @default.
- W2587479669 hasConcept C185592680 @default.
- W2587479669 hasConcept C2777998628 @default.
- W2587479669 hasConcept C2779830873 @default.
- W2587479669 hasConcept C2780117898 @default.
- W2587479669 hasConcept C2781338088 @default.
- W2587479669 hasConcept C41183919 @default.
- W2587479669 hasConcept C43617362 @default.
- W2587479669 hasConcept C53007507 @default.
- W2587479669 hasConcept C55493867 @default.
- W2587479669 hasConcept C87554066 @default.
- W2587479669 hasConceptScore W2587479669C178790620 @default.
- W2587479669 hasConceptScore W2587479669C181199279 @default.
- W2587479669 hasConceptScore W2587479669C185592680 @default.
- W2587479669 hasConceptScore W2587479669C2777998628 @default.
- W2587479669 hasConceptScore W2587479669C2779830873 @default.
- W2587479669 hasConceptScore W2587479669C2780117898 @default.
- W2587479669 hasConceptScore W2587479669C2781338088 @default.
- W2587479669 hasConceptScore W2587479669C41183919 @default.
- W2587479669 hasConceptScore W2587479669C43617362 @default.
- W2587479669 hasConceptScore W2587479669C53007507 @default.
- W2587479669 hasConceptScore W2587479669C55493867 @default.
- W2587479669 hasConceptScore W2587479669C87554066 @default.
- W2587479669 hasLocation W25874796691 @default.
- W2587479669 hasOpenAccess W2587479669 @default.
- W2587479669 hasPrimaryLocation W25874796691 @default.
- W2587479669 hasRelatedWork W1591346049 @default.
- W2587479669 hasRelatedWork W180756988 @default.
- W2587479669 hasRelatedWork W1846135429 @default.
- W2587479669 hasRelatedWork W1966787002 @default.
- W2587479669 hasRelatedWork W1966914647 @default.
- W2587479669 hasRelatedWork W1991461635 @default.
- W2587479669 hasRelatedWork W1992061933 @default.
- W2587479669 hasRelatedWork W2028920582 @default.
- W2587479669 hasRelatedWork W2036001630 @default.
- W2587479669 hasRelatedWork W2040875509 @default.
- W2587479669 hasRelatedWork W2055078360 @default.
- W2587479669 hasRelatedWork W2056585491 @default.
- W2587479669 hasRelatedWork W2077507754 @default.
- W2587479669 hasRelatedWork W2090664900 @default.
- W2587479669 hasRelatedWork W2154253002 @default.
- W2587479669 hasRelatedWork W2156940294 @default.
- W2587479669 hasRelatedWork W2355309456 @default.
- W2587479669 hasRelatedWork W2364127625 @default.
- W2587479669 hasRelatedWork W2386969500 @default.
- W2587479669 hasRelatedWork W2410464065 @default.
- W2587479669 isParatext "false" @default.
- W2587479669 isRetracted "false" @default.
- W2587479669 magId "2587479669" @default.
- W2587479669 workType "article" @default.