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- W2587752492 abstract "A new L-lactate selective impedimetric biosensor based on a lactate dehydrogenase (LDH)/pyruvate oxidase (PyrOx) bioselective membrane is reported, fabricated using enzyme drop coating and cross-linking via glutaraldehyde vapours. The sensing strategy is based on the measurement of the change in impedance caused by the generation of charged ions (CH3COO−, H+ and HCO3−), from PyrOx catalysed pyruvate oxidation, the pyruvate resulting from L-lactate oxidation catalysed by LDH + nicotinamide adenine dinucleotide (NAD+). The experimental conditions, pH and buffer concentration, were optimized for best biosensor response, and operational/storage stability and selectivity/specificity were investigated. The limit of detection for L-lactate, was 17 and 20 μM, considering changes in resistance and charge separation parameters at and close to the LDH-NAD+/PyrOx interface, respectively. The biosensor was successfully employed for the determination of L-lactate in yogurt samples." @default.
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- W2587752492 date "2017-03-01" @default.
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- W2587752492 title "L-lactate selective impedimetric bienzymatic biosensor based on lactate dehydrogenase and pyruvate oxidase" @default.
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- W2587752492 doi "https://doi.org/10.1016/j.electacta.2017.02.050" @default.
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