Matches in SemOpenAlex for { <https://semopenalex.org/work/W2587874852> ?p ?o ?g. }
Showing items 1 to 84 of
84
with 100 items per page.
- W2587874852 endingPage "212" @default.
- W2587874852 startingPage "209" @default.
- W2587874852 abstract "Objective To explore the correlation between the amount of residual undifferentiated embryonic stem cells (ESCs) in embryoid bodies and its tumorigenicity.Methods Mouse R1 ESCs were cultured in suspension to form embryoid bodies (EBs).Ten days later,EBs were digested into single cells and then re-plated in standard ESCs culture condition.The residual undifferentiated embryonic stem cells surface maker SSEA-1 was examined by flow cytometry in EBs.The morphology of residual undifferentiated cells in EBs were observed,meanwhile the surface marker SSEA-1 was examined by immunofluorescent staining.EBs were digested into single cells and grouped into 104,105,106,2×106,and then injected into limb muscle of nude mice.The correlation of the amount of cells and its tumorigenicity was observed.Results Residual undifferentiated ESCs were observed after EBs differentiated for 10 days,which displayed clonal morphology and expressed undifferentiated ceil markers of ESCs,such as SSEA-1.The expression rate of undifferentiated cells surface marker SSEA-1 was (13.5±0.75)％ in EBs differentiated for 10 days.Only two millions single cells harvested from EBs were able to form teratoma after being injected into muscle of nude mice for 6 weeks.Mature endoderm,mesoderm and ectoderm tissues could be found in teratoma.No teratoma formed in other groups.Conclusion A certain amount residual undifferentiated ESCs still exist after differentiation of ESCs into EBs.About 2.7× 105 undifferentiated cells are able to form teratoma by iniecting into muscle of nude mice.Key words: Embryonic stem cells; Embryoid bodies; SSEA-1; Teratoma" @default.
- W2587874852 created "2017-02-24" @default.
- W2587874852 creator A5001352589 @default.
- W2587874852 creator A5012507632 @default.
- W2587874852 creator A5029276357 @default.
- W2587874852 creator A5037176979 @default.
- W2587874852 creator A5067767564 @default.
- W2587874852 date "2012-08-28" @default.
- W2587874852 modified "2023-09-24" @default.
- W2587874852 title "Correlation between the amount of residual undifferentiated embryonic stem cells in embryoid bodies and its tumorigenicity" @default.
- W2587874852 doi "https://doi.org/10.3760/cma.j.issn.1673-4181.2012.04.005" @default.
- W2587874852 hasPublicationYear "2012" @default.
- W2587874852 type Work @default.
- W2587874852 sameAs 2587874852 @default.
- W2587874852 citedByCount "0" @default.
- W2587874852 crossrefType "journal-article" @default.
- W2587874852 hasAuthorship W2587874852A5001352589 @default.
- W2587874852 hasAuthorship W2587874852A5012507632 @default.
- W2587874852 hasAuthorship W2587874852A5029276357 @default.
- W2587874852 hasAuthorship W2587874852A5037176979 @default.
- W2587874852 hasAuthorship W2587874852A5067767564 @default.
- W2587874852 hasConcept C104317684 @default.
- W2587874852 hasConcept C142724271 @default.
- W2587874852 hasConcept C143948831 @default.
- W2587874852 hasConcept C145103041 @default.
- W2587874852 hasConcept C185592680 @default.
- W2587874852 hasConcept C196843134 @default.
- W2587874852 hasConcept C20875687 @default.
- W2587874852 hasConcept C2776057878 @default.
- W2587874852 hasConcept C2777164004 @default.
- W2587874852 hasConcept C2779329299 @default.
- W2587874852 hasConcept C28328180 @default.
- W2587874852 hasConcept C55493867 @default.
- W2587874852 hasConcept C71924100 @default.
- W2587874852 hasConcept C86803240 @default.
- W2587874852 hasConcept C87073359 @default.
- W2587874852 hasConcept C95444343 @default.
- W2587874852 hasConceptScore W2587874852C104317684 @default.
- W2587874852 hasConceptScore W2587874852C142724271 @default.
- W2587874852 hasConceptScore W2587874852C143948831 @default.
- W2587874852 hasConceptScore W2587874852C145103041 @default.
- W2587874852 hasConceptScore W2587874852C185592680 @default.
- W2587874852 hasConceptScore W2587874852C196843134 @default.
- W2587874852 hasConceptScore W2587874852C20875687 @default.
- W2587874852 hasConceptScore W2587874852C2776057878 @default.
- W2587874852 hasConceptScore W2587874852C2777164004 @default.
- W2587874852 hasConceptScore W2587874852C2779329299 @default.
- W2587874852 hasConceptScore W2587874852C28328180 @default.
- W2587874852 hasConceptScore W2587874852C55493867 @default.
- W2587874852 hasConceptScore W2587874852C71924100 @default.
- W2587874852 hasConceptScore W2587874852C86803240 @default.
- W2587874852 hasConceptScore W2587874852C87073359 @default.
- W2587874852 hasConceptScore W2587874852C95444343 @default.
- W2587874852 hasIssue "04" @default.
- W2587874852 hasLocation W25878748521 @default.
- W2587874852 hasOpenAccess W2587874852 @default.
- W2587874852 hasPrimaryLocation W25878748521 @default.
- W2587874852 hasRelatedWork W166309462 @default.
- W2587874852 hasRelatedWork W1984711934 @default.
- W2587874852 hasRelatedWork W1988710301 @default.
- W2587874852 hasRelatedWork W2031280968 @default.
- W2587874852 hasRelatedWork W2053019739 @default.
- W2587874852 hasRelatedWork W2075855216 @default.
- W2587874852 hasRelatedWork W2101568772 @default.
- W2587874852 hasRelatedWork W2159675355 @default.
- W2587874852 hasRelatedWork W2353189380 @default.
- W2587874852 hasRelatedWork W2378461215 @default.
- W2587874852 hasRelatedWork W2384095366 @default.
- W2587874852 hasRelatedWork W2387494959 @default.
- W2587874852 hasRelatedWork W2389607431 @default.
- W2587874852 hasRelatedWork W2391233314 @default.
- W2587874852 hasRelatedWork W2394017403 @default.
- W2587874852 hasRelatedWork W2406466138 @default.
- W2587874852 hasRelatedWork W2798042600 @default.
- W2587874852 hasRelatedWork W343897009 @default.
- W2587874852 hasRelatedWork W624690974 @default.
- W2587874852 hasRelatedWork W2941954150 @default.
- W2587874852 hasVolume "35" @default.
- W2587874852 isParatext "false" @default.
- W2587874852 isRetracted "false" @default.
- W2587874852 magId "2587874852" @default.
- W2587874852 workType "article" @default.