FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2587881227> ?p ?o ?g. }

• W2587881227 abstract "Abstract Abstract 5155 Patients with newly diagnosed essential thrombocythemia (ET) were investigated for selective accumulation/expansion of CD4+ lymphocytes subpopulations in the marrow, to verifie the hypothesis that the neoplastic process associated factor(s) are recognized by the immune system. Sixteen patients (F/M: 12/4, age 28–72 - median 60 yrs) with ET diagnosed according to the WHO criteria were studied. They had platelets from 496–809 x 10̂3/ul (median 644 × 10̂3 ), (12 heterozygotes, 2 homozygotes). Cytological evaluation revealed normal differentiation of erythroid and myeloid lineages, in contrast an increased number of megakaryocytes often enlarged, hyperlobulated and in clusters were seen in all patients. Trephine biopsy revealed activation of megakaryopoiesis with enlarged numerous megakaryocytes with normal representation of other lineages and a lack of an increase in the reticulin fibers. Marrow cellularity was from 9 – 126 x10̂3/ul (median 42 × 10̂3/ul). The whole populations of marrow and blood cells were labeled for a purpose of this study with the use CD4, CD25, CD45RO and CCR7 MoAb (Becton Dickinson, San Jose, CA, UmarSA) followed by detection and analysis with the use of flow cytometry (FACS Calibur, Becton Dickinson) equipped with PCLysis programe. Immunostaining of trephine biopsies was used for detection of FOXP3 cells. It was found: (i) Lymphocytes in the marrow were in proportions from 4,5%-15,7% (median 7,25%), in numbers from1377/ul to 8245/ul (median 3870/ul) what was significantly higher as compared to blood from 1300/ul to 3900/ul (median 2100/ul) (Wilcoxon Matched Pairs Test, p=0.010). (ii) CD45RO+CCR7- lymphocytes were higher in numbers in the marrow aspirates as compared to blood (median 1281/ul vs 631,2/ul, p=0,04 Wilcoxon Test for pairs). (iii) In contrast numbers in the marrow aspirates of CD45 RO-CCR7+ (median 297,4/ul vs median 238,2/ul), as well as CD45 RO+CCR7+ (median 149,6/ul vs 146,4/ul) did not differ from those in blood. (iv) It was a significant increase of CD4+CD25high+ cells in the marrow aspirates as compared to blood (median 36,2/ul vs 9,1/ul; p=0,02 Wilcoxon Test for pairs). Indeed in 11 out of 14 trephine biopsies investigated FoxP3+ cells were identified. In conclusion an increase, in the marrow of ET patients, of effector/memory CD4 lymphocytes (CD45RO+CCR7-) but not those of naïve and central memory lymphocytes phenotypes points out on the selective accumulation of these cells in the marrows. Concomitant increase of T regulatory cells (CD4+CD25+high) suggests that an active immunological is taking place at the site. It is possible that effector memory cells are attracted to come to the marrows by ET process associated factors and if so the present observation constitute the primary observation building the rationale behind immunotherapy in ET patients Disclosures: No relevant conflicts of interest to declare." @default.
• W2587881227 created "2017-02-24" @default.
• W2587881227 creator A5002719895 @default.
• W2587881227 creator A5009074459 @default.
• W2587881227 creator A5047267675 @default.
• W2587881227 creator A5058681080 @default.
• W2587881227 date "2011-11-18" @default.
• W2587881227 modified "2023-09-27" @default.
• W2587881227 title "Selective Accumulation of CD45RO+CCR7- in the Marrow Aspirates in Patients with Essential Thrombocythemia" @default.
• W2587881227 doi "https://doi.org/10.1182/blood.v118.21.5155.5155" @default.
• W2587881227 hasPublicationYear "2011" @default.
• W2587881227 type Work @default.
• W2587881227 sameAs 2587881227 @default.
• W2587881227 citedByCount "0" @default.
• W2587881227 crossrefType "journal-article" @default.
• W2587881227 hasAuthorship W2587881227A5002719895 @default.
• W2587881227 hasAuthorship W2587881227A5009074459 @default.
• W2587881227 hasAuthorship W2587881227A5047267675 @default.
• W2587881227 hasAuthorship W2587881227A5058681080 @default.
• W2587881227 hasConcept C109159458 @default.
• W2587881227 hasConcept C142724271 @default.
• W2587881227 hasConcept C203014093 @default.
• W2587881227 hasConcept C2779282312 @default.
• W2587881227 hasConcept C2779705218 @default.
• W2587881227 hasConcept C2780007613 @default.
• W2587881227 hasConcept C2780076729 @default.
• W2587881227 hasConcept C2781057849 @default.
• W2587881227 hasConcept C28328180 @default.
• W2587881227 hasConcept C54355233 @default.
• W2587881227 hasConcept C71924100 @default.
• W2587881227 hasConcept C86803240 @default.
• W2587881227 hasConcept C89560881 @default.
• W2587881227 hasConceptScore W2587881227C109159458 @default.
• W2587881227 hasConceptScore W2587881227C142724271 @default.
• W2587881227 hasConceptScore W2587881227C203014093 @default.
• W2587881227 hasConceptScore W2587881227C2779282312 @default.
• W2587881227 hasConceptScore W2587881227C2779705218 @default.
• W2587881227 hasConceptScore W2587881227C2780007613 @default.
• W2587881227 hasConceptScore W2587881227C2780076729 @default.
• W2587881227 hasConceptScore W2587881227C2781057849 @default.
• W2587881227 hasConceptScore W2587881227C28328180 @default.
• W2587881227 hasConceptScore W2587881227C54355233 @default.
• W2587881227 hasConceptScore W2587881227C71924100 @default.
• W2587881227 hasConceptScore W2587881227C86803240 @default.
• W2587881227 hasConceptScore W2587881227C89560881 @default.
• W2587881227 hasLocation W25878812271 @default.
• W2587881227 hasOpenAccess W2587881227 @default.
• W2587881227 hasPrimaryLocation W25878812271 @default.
• W2587881227 hasRelatedWork W134790080 @default.
• W2587881227 hasRelatedWork W155144104 @default.
• W2587881227 hasRelatedWork W165456120 @default.
• W2587881227 hasRelatedWork W168228701 @default.
• W2587881227 hasRelatedWork W1987280240 @default.
• W2587881227 hasRelatedWork W2104891424 @default.
• W2587881227 hasRelatedWork W2405316906 @default.
• W2587881227 hasRelatedWork W2406238319 @default.
• W2587881227 hasRelatedWork W2411314248 @default.
• W2587881227 hasRelatedWork W2460109688 @default.
• W2587881227 hasRelatedWork W2543358499 @default.
• W2587881227 hasRelatedWork W2554268079 @default.
• W2587881227 hasRelatedWork W2560306455 @default.
• W2587881227 hasRelatedWork W2585732636 @default.
• W2587881227 hasRelatedWork W2587045623 @default.
• W2587881227 hasRelatedWork W2970961861 @default.
• W2587881227 hasRelatedWork W2980340848 @default.
• W2587881227 hasRelatedWork W2999661765 @default.
• W2587881227 hasRelatedWork W3030722814 @default.
• W2587881227 hasRelatedWork W3178693185 @default.
• W2587881227 isParatext "false" @default.
• W2587881227 isRetracted "false" @default.
• W2587881227 magId "2587881227" @default.
• W2587881227 workType "article" @default.