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- W2588075470 abstract "Severe fever with thrombocytopenia syndrome (SFTS) caused by SFTS virus (SFTSV) usually have a high fatality. At present no effective therapy or vaccine are available, so early diagnosis of SFTS is crucial to prevent and control SFTSV infection. This study aimed to establish a national reference for these diagnostic kits of SFTSV genome and make the diagnosis of the disease effective. Six SFTSV strains isolated from different regions, and five relative viruses with similar clinical manifestations were selected as positive and negative references and assessed using real time quantitative PCR (q-PCR) using specific primers and probe and two commercial kits. The stability of the references was also assessed at 37 °C, room temperature or -70 °C for 8 days, 14 days or 8 months respectively, or following several cycles of freezing-thawing. Collaborative calibration of the references was performed by three labs. The references indicated good accuracy and specificity. The lowest detection limit was 102 U/mL. The accuracy was coefficient of variation less than 5%. The references were highly stable at high temperatures and after long storing and freezing-thawing treatment. We successfully established a national reference with good accuracy, high specificity, sensitivity and stability, which can be applied for quality control of commercial SFTSV diagnostic kits, thus preventing and controlling SFTS. The references have been finished and it was retrospectively registered in the following article." @default.
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- W2588075470 date "2017-02-16" @default.
- W2588075470 modified "2023-09-27" @default.
- W2588075470 title "Establishment of national reference for bunyavirus nucleic acid detection kits for diagnosis of SFTS virus" @default.
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- W2588075470 doi "https://doi.org/10.1186/s12985-017-0682-z" @default.
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