FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W2588200059> ?p ?o ?g. }

• W2588200059 abstract "D-Glucuronic acid is a biomass component that occurs in plant cell wall polysaccharides and is catabolized by saprotrophic microorganisms including fungi. A pathway for D-glucuronic acid catabolism in fungal microorganisms is only partly known. In the filamentous fungus Aspergillus niger, the enzymes that are known to be part of the pathway are the NADPH requiring D-glucuronic acid reductase forming L-gulonate and the NADH requiring 2-keto-L-gulonate reductase that forms L-idonate. With the aid of RNA sequencing we identified two more enzymes of the pathway. The first is a NADPH requiring 2-keto-L-gulonate reductase that forms L-idonate, GluD. The second is a NAD+ requiring L-idonate 5-dehydrogenase forming 5-keto-gluconate, GluE. The genes coding for these two enzymes are clustered and share the same bidirectional promoter. The GluD is an enzyme with a strict requirement for NADP+/NADPH as cofactors. The kcat for 2-keto-L-gulonate and L-idonate is 21.4 and 1.1 s-1, and the Km 25.3 and 12.6 mM, respectively, when using the purified protein. In contrast, the GluE has a strict requirement for NAD+/NADH. The kcat for L-idonate and 5-keto-D-gluconate is 5.5 and 7.2 s-1, and the Km 30.9 and 8.4 mM, respectively. These values also refer to the purified protein. The gluD deletion resulted in accumulation of 2-keto-L-gulonate in the liquid cultivation while the gluE deletion resulted in reduced growth and cessation of the D-glucuronic acid catabolism." @default.
• W2588200059 created "2017-02-24" @default.
• W2588200059 creator A5006250868 @default.
• W2588200059 creator A5036297981 @default.
• W2588200059 creator A5063902514 @default.
• W2588200059 date "2017-02-14" @default.
• W2588200059 modified "2023-10-18" @default.
• W2588200059 title "Clustered Genes Encoding 2-Keto-l-Gulonate Reductase and l-Idonate 5-Dehydrogenase in the Novel Fungal d-Glucuronic Acid Pathway" @default.
• W2588200059 cites W1509314550 @default.
• W2588200059 cites W151023673 @default.
• W2588200059 cites W1603319631 @default.
• W2588200059 cites W1857433864 @default.
• W2588200059 cites W1965381930 @default.
• W2588200059 cites W1990612688 @default.
• W2588200059 cites W1996641651 @default.
• W2588200059 cites W2005850001 @default.
• W2588200059 cites W2005914130 @default.
• W2588200059 cites W2009499636 @default.
• W2588200059 cites W2038591876 @default.
• W2588200059 cites W2038946558 @default.
• W2588200059 cites W2075687569 @default.
• W2588200059 cites W2075814472 @default.
• W2588200059 cites W2081592239 @default.
• W2588200059 cites W2086514413 @default.
• W2588200059 cites W2087550487 @default.
• W2588200059 cites W2094514812 @default.
• W2588200059 cites W2101421252 @default.
• W2588200059 cites W2104485225 @default.
• W2588200059 cites W2105227785 @default.
• W2588200059 cites W2111831499 @default.
• W2588200059 cites W2119900778 @default.
• W2588200059 cites W2121004444 @default.
• W2588200059 cites W2121785567 @default.
• W2588200059 cites W2132077776 @default.
• W2588200059 cites W2133317164 @default.
• W2588200059 cites W2157407884 @default.
• W2588200059 cites W2159684200 @default.
• W2588200059 cites W2396986089 @default.
• W2588200059 cites W288157794 @default.
• W2588200059 cites W927857605 @default.
• W2588200059 cites W958219903 @default.
• W2588200059 cites W995731482 @default.
• W2588200059 doi "https://doi.org/10.3389/fmicb.2017.00225" @default.
• W2588200059 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/5306355" @default.
• W2588200059 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/28261181" @default.
• W2588200059 hasPublicationYear "2017" @default.
• W2588200059 type Work @default.
• W2588200059 sameAs 2588200059 @default.
• W2588200059 citedByCount "18" @default.
• W2588200059 countsByYear W25882000592017 @default.
• W2588200059 countsByYear W25882000592018 @default.
• W2588200059 countsByYear W25882000592019 @default.
• W2588200059 countsByYear W25882000592020 @default.
• W2588200059 countsByYear W25882000592021 @default.
• W2588200059 countsByYear W25882000592022 @default.
• W2588200059 crossrefType "journal-article" @default.
• W2588200059 hasAuthorship W2588200059A5006250868 @default.
• W2588200059 hasAuthorship W2588200059A5036297981 @default.
• W2588200059 hasAuthorship W2588200059A5063902514 @default.
• W2588200059 hasBestOaLocation W25882000591 @default.
• W2588200059 hasConcept C100817775 @default.
• W2588200059 hasConcept C104317684 @default.
• W2588200059 hasConcept C134651460 @default.
• W2588200059 hasConcept C143065580 @default.
• W2588200059 hasConcept C181199279 @default.
• W2588200059 hasConcept C185592680 @default.
• W2588200059 hasConcept C197957613 @default.
• W2588200059 hasConcept C2775960407 @default.
• W2588200059 hasConcept C2776317432 @default.
• W2588200059 hasConcept C2776648616 @default.
• W2588200059 hasConcept C2776923806 @default.
• W2588200059 hasConcept C41183919 @default.
• W2588200059 hasConcept C55493867 @default.
• W2588200059 hasConcept C56856141 @default.
• W2588200059 hasConcept C71240020 @default.
• W2588200059 hasConcept C75520062 @default.
• W2588200059 hasConcept C86803240 @default.
• W2588200059 hasConcept C96942376 @default.
• W2588200059 hasConceptScore W2588200059C100817775 @default.
• W2588200059 hasConceptScore W2588200059C104317684 @default.
• W2588200059 hasConceptScore W2588200059C134651460 @default.
• W2588200059 hasConceptScore W2588200059C143065580 @default.
• W2588200059 hasConceptScore W2588200059C181199279 @default.
• W2588200059 hasConceptScore W2588200059C185592680 @default.
• W2588200059 hasConceptScore W2588200059C197957613 @default.
• W2588200059 hasConceptScore W2588200059C2775960407 @default.
• W2588200059 hasConceptScore W2588200059C2776317432 @default.
• W2588200059 hasConceptScore W2588200059C2776648616 @default.
• W2588200059 hasConceptScore W2588200059C2776923806 @default.
• W2588200059 hasConceptScore W2588200059C41183919 @default.
• W2588200059 hasConceptScore W2588200059C55493867 @default.
• W2588200059 hasConceptScore W2588200059C56856141 @default.
• W2588200059 hasConceptScore W2588200059C71240020 @default.
• W2588200059 hasConceptScore W2588200059C75520062 @default.
• W2588200059 hasConceptScore W2588200059C86803240 @default.
• W2588200059 hasConceptScore W2588200059C96942376 @default.
• W2588200059 hasFunder F4320321108 @default.
• W2588200059 hasLocation W25882000591 @default.
• W2588200059 hasLocation W25882000592 @default.
• W2588200059 hasLocation W25882000593 @default.

• next